Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,493,653	G→T	6.9%	L184M (CTG→ATG)	PP_3097 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,493,653	0	G	T	6.9%	270.4 / 6.5	85	L184M (CTG→ATG)	PP_3097	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/42);  new base T (4/2);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CCCACGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAATCGGCCACA  >  NC_002947/3493505‑3493799                                                                                                                                                     |                                                                                                                                                   cccACGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTTAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAg                                                                                                                                                    >  7:76250/1‑149 (MQ=255)  ccACGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCCGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAgg                                                                                                                                                   >  3:391743/1‑149 (MQ=255)    aCGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCg                                                                                                                                                 >  8:363312/1‑149 (MQ=255)    aCGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCg                                                                                                                                                 <  7:9388/149‑1 (MQ=255)    aCGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCg                                                                                                                                                 <  7:47799/149‑1 (MQ=255)     cGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCgg                                                                                                                                                <  5:73543/149‑1 (MQ=255)      ggTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGt                                                                                                                                               <  2:63535/149‑1 (MQ=255)       gTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGtt                                                                                                                                              <  8:76250/149‑1 (MQ=255)            cAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATg                                                                                                                                          <  7:299339/148‑1 (MQ=255)             aGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTc                                                                                                                                        >  7:206416/1‑149 (MQ=255)              gggCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTc                                                                                                                                        >  7:122959/1‑148 (MQ=255)               ggCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGtt                                                                                                                                              <  2:30686/141‑1 (MQ=255)               ggCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGtt                                                                                                                                              >  1:30686/1‑141 (MQ=255)               ggCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATg                                                                                                                                          <  6:360310/145‑1 (MQ=255)               ggCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATg                                                                                                                                          >  5:360310/1‑145 (MQ=255)                gCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCAt                                                                                                                                     <  7:257677/149‑1 (MQ=255)                  aTCGGTCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCTTCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCt                                                                                                                                   >  2:246509/1‑149 (MQ=255)                      gccggcAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGccc                                                                                                                               <  2:368099/149‑1 (MQ=255)                         ggcAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGaa                                                                                                                            >  3:56034/1‑149 (MQ=255)                            aGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAg                                                                                                                         <  2:328534/149‑1 (MQ=255)                             gcgcCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAgg                                                                                                                        <  7:363312/149‑1 (MQ=255)                              cgcCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCCTGCGGTTcttc                                                                                                                                          >  7:319839/1‑127 (MQ=255)                              cgcCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCCTGCGGTTcttc                                                                                                                                          <  8:319839/130‑4 (MQ=255)                                  aCCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGGTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGATCAGGGCATc                                                                                                                   >  3:233399/1‑149 (MQ=255)                                        cgGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTAATGACCATCTGCCCGAACAGGGCATCGCCAGt                                                                                                             >  5:30421/1‑149 (MQ=255)                                         ggCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTg                                                                                                            <  5:215841/149‑1 (MQ=255)                                              cGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCt                                                                                                         <  1:293433/147‑1 (MQ=255)                                                gCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCa                                                                                                     <  2:92018/149‑1 (MQ=255)                                                  ccTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAgg                                                                                                   >  1:3338/1‑149 (MQ=255)                                                   cTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGc                                                                                                  <  6:311660/149‑1 (MQ=255)                                                   cTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGc                                                                                                  >  5:284849/1‑149 (MQ=255)                                                      gtcgtcGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCg                                                                                                 <  4:73948/147‑1 (MQ=255)                                                         gtcgAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATgc                                                                                            >  8:119895/1‑149 (MQ=255)                                                         gtcgAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATgc                                                                                            >  6:344837/1‑149 (MQ=255)                                                         gtcgAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATgc                                                                                            <  5:261742/149‑1 (MQ=255)                                                          tcgAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATgcg                                                                                           >  3:328953/1‑149 (MQ=255)                                                              aGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGa                                                                                       <  4:328953/149‑1 (MQ=255)                                                              aGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGa                                                                                       >  1:30069/1‑149 (MQ=255)                                                               gCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGAc                                                                                      >  7:329264/1‑149 (MQ=255)                                                                 tCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGAATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGAAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTTCCAGGCGGATGCGCAGAc                                                                                      >  5:188402/1‑147 (MQ=255)                                                                        ggCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCa                                                                             <  4:338638/149‑1 (MQ=255)                                                                          ccTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAgg                                                                           >  4:202616/1‑149 (MQ=255)                                                                           cTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGt                                                                          >  6:376105/1‑149 (MQ=255)                                                                           cTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGt                                                                          >  5:365937/1‑149 (MQ=255)                                                                              ggCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCgg                                                                       >  3:352644/1‑149 (MQ=255)                                                                               gCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGgc                                                                      <  2:3338/149‑1 (MQ=255)                                                                                 gAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCATGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGgcgc                                                                    <  6:319530/149‑1 (MQ=255)                                                                                 gAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGgcgc                                                                    >  2:24622/1‑149 (MQ=255)                                                                                   gtgtgttgAACAGCCGTGAGTACAGCTGCAGCAGCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCCTCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCtg                                                                  <  4:391743/146‑1 (MQ=255)                                                                                     ttgttgAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCtgtg                                                                >  8:302449/1‑149 (MQ=255)                                                                                          gAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGt                                                           >  4:338489/1‑149 (MQ=255)                                                                                           aaCAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGt                                                           <  6:365937/148‑1 (MQ=255)                                                                                              agcagTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTg                                                         >  3:375847/1‑147 (MQ=255)                                                                                              agcagTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGaa                                                       <  7:119895/149‑1 (MQ=255)                                                                                               gcagTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAg                                                      <  8:206416/149‑1 (MQ=255)                                                                                                   ttCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGAGAATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCg                                                  >  7:252153/1‑149 (MQ=255)                                                                                                      gTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGc                                               >  8:241721/1‑149 (MQ=255)                                                                                                       tACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTACCCGTTGAAGGCCGAGCg                                              >  5:20053/1‑149 (MQ=255)                                                                                                        aCAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGc                                                                                  >  2:89474/1‑112 (MQ=255)                                                                                                        aCAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGc                                                                                  <  1:89474/112‑1 (MQ=255)                                                                                                           gCTGCAGCATCAGGGGCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTcc                                          <  4:233399/149‑1 (MQ=255)                                                                                                            cTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCa                                         >  4:40054/1‑149 (MQ=255)                                                                                                             tGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCAt                                        >  7:272676/1‑149 (MQ=255)                                                                                                                 gcaTCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCt                                    <  3:253098/149‑1 (MQ=255)                                                                                                                 gcaTCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCt                                    <  5:344837/149‑1 (MQ=255)                                                                                                                 gcaTCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCt                                    <  4:352644/149‑1 (MQ=255)                                                                                                                    tCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCtgtg                                 <  8:329264/149‑1 (MQ=255)                                                                                                                       gggTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAAc                              <  7:317782/149‑1 (MQ=255)                                                                                                                          tCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAg                           <  1:246509/149‑1 (MQ=255)                                                                                                                          tCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAg                           <  3:338489/149‑1 (MQ=255)                                                                                                                             cTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGcc                        >  5:96730/1‑149 (MQ=255)                                                                                                                                cGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATTCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCct                       <  8:272676/147‑1 (MQ=255)                                                                                                                                cGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGAGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCct                       <  3:116943/147‑1 (MQ=255)                                                                                                                                 gCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGActgct                    >  8:243503/1‑149 (MQ=255)                                                                                                                                    gTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGACCAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCa                 <  4:56034/149‑1 (MQ=255)                                                                                                                                     tCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCAc                <  1:24622/149‑1 (MQ=255)                                                                                                                                     tCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCAc                <  3:40054/149‑1 (MQ=255)                                                                                                                                        aGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACgg              >  8:297225/1‑148 (MQ=255)                                                                                                                                        aGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACgg              >  8:55171/1‑148 (MQ=255)                                                                                                                                          gaagaaCCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCaa           >  1:26479/1‑149 (MQ=255)                                                                                                                                          gaagaaCCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCaa           >  1:20368/1‑149 (MQ=255)                                                                                                                                           atgaaCCGCCTGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTTTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAAt          >  1:302638/3‑149 (MQ=255)                                                                                                                                           atgaaCCGCCTGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCaa           >  8:29609/3‑148 (MQ=255)                                                                                                                                           atgaaCCGCCTGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAAt          >  5:33916/3‑149 (MQ=255)                                                                                                                                                 cGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAATCGGCca    <  2:20368/149‑1 (MQ=255)                                                                                                                                                 cGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAATCGGCca    <  2:26479/149‑1 (MQ=255)                                                                                                                                                   cAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAATCGGCcaca  <  5:376105/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCCACGGTTTTCAGGGCATCGGCCGGCAGCGCCACCTCGCGGCTGCGGCCCTGGTCGTCGAAGCTCAGCGTGGCCTTGGCGAGGTTGTTGAACAGCAGTTCGTACAGCTGCAGCATCAGGGTCGCTTCGCCGTCGAGGAAGAACCGCAGGCGGTTCATGTCCATCTGCCCGAACAGGGCATCGCCAGTGGCTGCCAGGCGGATGCGCAGACGAGCGACCAGGTCGGCGCTGTGCCCGTTGAAGGCCGAGCGTTCCATTTCTGTGAACGAGGCCTGCTGCACGGCAATCGGCCACA  >  NC_002947/3493505‑3493799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑