Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,520,081 T→A 8.8% intergenic (+700/‑180) PP_5580 → / → PP_5582 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,520,0810TA 8.8% 155.3 / 2.7 54intergenic (+700/‑180)PP_5580/PP_5582hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base A (2/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATGAAGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGATTGTTTGTTGTGTC  >  NC_002947/3519939‑3520228
                                                                                                                                              |                                                                                                                                                    
aCGATGAAGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAAGCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATgc                                                                                                                                                 >  1:283831/1‑148 (MQ=255)
      aaGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATTTTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCATAagcaagcat                                                                                                                                           <  4:29996/148‑2 (MQ=255)
       aGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATa                                                                                                                                         <  3:207083/149‑1 (MQ=255)
       aGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCATAagcaagcatt                                                                                                                                          <  5:59264/148‑3 (MQ=255)
        ggAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAg                                                                                                                                        >  5:183357/1‑149 (MQ=255)
         gAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGt                                                                                                                                       >  2:96172/1‑149 (MQ=255)
           aaGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCATAagcaagcattgaga                                                                                                                                      >  5:317367/1‑142 (MQ=255)
           aaGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCATAagcaagcatt                                                                                                                                          <  6:317367/144‑3 (MQ=255)
             ggCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTc                                                                                                                                   <  3:193721/149‑1 (MQ=255)
             ggCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCATAagcaagca                                                                                                                                            >  4:250214/1‑140 (MQ=255)
               cTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCt                                                                                                                                 >  8:118879/1‑149 (MQ=255)
                  ttAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTgg                                                                                                                               <  7:312964/148‑1 (MQ=255)
                       gCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAg                                                                                                                          >  3:287118/1‑148 (MQ=255)
                            tGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAatat                                                                                                                    >  2:122112/1‑149 (MQ=255)
                                gTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCAt                                                                                                                <  3:20077/149‑1 (MQ=255)
                                 tCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCAtt                                                                                                               <  3:347610/149‑1 (MQ=255)
                                   atatTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGa                                                                                                             <  6:37979/149‑1 (MQ=255)
                                           gaATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATg                                                                                                    <  4:322201/149‑1 (MQ=255)
                                           gaATCGAGTCCCAGATATATCATGCCGCC‑GGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATgg                                                                                                   >  6:234809/1‑149 (MQ=255)
                                                     ccAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGt                                                                                          <  1:38962/149‑1 (MQ=255)
                                                      cAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTTCTACGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTg                                                                                         <  3:7973/149‑1 (MQ=255)
                                                      cAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTg                                                                                         >  4:252496/1‑149 (MQ=255)
                                                      cAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCATGGTACAGAAATATTCATTGAGCGAG‑ATGTCGAAAGCGTg                                                                                         >  5:272421/1‑149 (MQ=255)
                                                        gATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGt                                                                                       <  1:34936/149‑1 (MQ=255)
                                                        gATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGt                                                                                       >  2:34936/1‑149 (MQ=255)
                                                            tatCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGc                                                                                   <  2:283831/149‑1 (MQ=255)
                                                                   ccgccTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGaaa                                                                                              <  8:63953/131‑1 (MQ=255)
                                                                          gaggaCGATCGTTAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTAc                                                                       <  4:299047/147‑1 (MQ=255)
                                                                           aggaCGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGAc                                                                    <  5:234809/149‑1 (MQ=255)
                                                                             gaCGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACa                                                                   >  8:371434/1‑148 (MQ=255)
                                                                             gaCGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACa                                                                   >  3:82708/1‑148 (MQ=255)
                                                                                      gAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGa                                                                     >  4:273546/1‑137 (MQ=255)
                                                                                      gAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGa                                                                     <  3:273546/137‑1 (MQ=255)
                                                                                      gAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCaa                                                          >  7:228302/1‑148 (MQ=255)
                                                                                       aaTATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCaa                                                          >  2:253454/1‑147 (MQ=255)
                                                                                             ggTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCa                                                   <  1:256366/148‑1 (MQ=255)
                                                                                                  tATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTa                                             >  1:161107/1‑149 (MQ=255)
                                                                                                   aTGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCaaaa                                                        >  4:172138/1‑137 (MQ=255)
                                                                                                   aTGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCaaaa                                                        <  3:172138/137‑1 (MQ=255)
                                                                                                   aTGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTa                                             >  7:312367/1‑148 (MQ=255)
                                                                                                    tGAATATAGTTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTGCTTGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAACTTCATAAGTGCACCTTTAc                                            <  6:216754/148‑1 (MQ=255)
                                                                                                      aaTATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACaaa                                         <  6:183357/149‑1 (MQ=255)
                                                                                                        tataGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAg                                        >  5:231767/1‑148 (MQ=255)
                                                                                                            ggTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAg                                   <  4:287118/149‑1 (MQ=255)
                                                                                                             gtgtGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGc                                  >  5:118719/1‑149 (MQ=255)
                                                                                                                tgAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAGCTTTGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCt                                 <  1:122112/148‑1 (MQ=255)
                                                                                                                  aaGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTc                              <  6:118719/148‑1 (MQ=255)
                                                                                                                       gcaCGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAAtt                         >  8:191095/1‑148 (MQ=255)
                                                                                                                       gcaCGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAAtt                         >  8:196627/1‑148 (MQ=255)
                                                                                                                          cGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGCCAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATg                     <  6:231767/149‑1 (MQ=255)
                                                                                                                                 tATCAATGCTTGCTTATTCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTTGTTGGGTTTTATTTTTACGACATAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTAt                      <  3:356793/141‑1 (MQ=255)
                                                                                                                                 tATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATATTTAAGACAAAAATTCAAAAGTGCACCTTTACAAAGTATAGCTTTCGAATTTAt                      >  4:356793/1‑141 (MQ=255)
                                                                                                                                 cactattgcttgcaTAAGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGATGTTATGCGTGAtt              >  4:329367/7‑149 (MQ=255)
                                                                                                                                    tattgcttgcaTAAGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGAttgtt           >  7:172328/4‑149 (MQ=255)
                                                                                                                                    cAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGAttgtt           >  2:218043/1‑149 (MQ=255)
                                                                                                                                    cAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGAttgtt           >  7:68164/1‑149 (MQ=255)
                                                                                                                                          ttgcttATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCGTTACAAAGTCGAGCGGTCGAATTTATGCGTGAgtgttttgtgt     >  8:262990/1‑144 (MQ=255)
                                                                                                                                             cttATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGATTGTTTGTTGTGtc  >  2:241966/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
ACGATGAAGGAAAGGCTATTAGGGCGTCTGGGGTCATATTTGAGAATCGAGTCCCAGATATATCATGCCGCCTGGAGGACGATGGTGAATATTGGTGCTATGAATATAGGTGTGAAGCAGCACGGATAGTATCAATGCTTGCTTATGCAAGCAATAGTGCTCCTGGTACAGAAATATTCATTGAGCGAG‑ATGGCGAAAGCGTGGTTGGCTTGTATTTTTACGACAAAAATTCAAAAGTGCACCTTTACAAAGTCTAGCTGTCGAATTTATGCGTGATTGTTTGTTGTGTC  >  NC_002947/3519939‑3520228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑