Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,580,628 A→G 5.5% I255V (ATC→GTC) ‡ benR → BenABC operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,580,6280AG 5.5% 211.6 / 2.4 72I255V (ATC→GTC) ‡benRBenABC operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base G (2/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

CCTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGGC  >  NC_002947/3580483‑3580775
                                                                                                                                                 |                                                                                                                                                   
ccTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATc                                                                                                                                                   <  1:338224/148‑1 (MQ=255)
 cTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCAt                                                                                                                                                    >  6:283515/1‑146 (MQ=255)
 cTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCAt                                                                                                                                                    <  5:283515/146‑1 (MQ=255)
       gCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGccc                                                                                                                                             >  2:378852/1‑147 (MQ=255)
         cccGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAg                                                                                                                                          >  6:288038/1‑148 (MQ=255)
          ccGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCa                                                                                                                                        >  7:321809/1‑149 (MQ=255)
           cGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCGTGATGCCCAAgtgc                                                                                                                                       <  5:240687/149‑4 (MQ=255)
           cGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCAt                                                                                                                                       >  5:60236/1‑149 (MQ=255)
            gCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCGTGATGCCCAAgtgct                                                                                                                                      >  4:157230/1‑145 (MQ=255)
             cAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCGTGATGCCCAAgtgctg                                                                                                                                     <  3:157230/149‑6 (MQ=255)
              aGGCTGGGCTATAGCGCATTCTTGATTACATCGAACGCAACCTTAAATTGGAATTGTCGGCTGAAGTGCTGGCATAGCAGGCTTGAATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCATTCGGGCATCACGCCCAAGCATTAc                                                                                                                                    <  7:246644/149‑1 (MQ=255)
                    ggCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTgc                                                                                                                                <  8:313672/147‑1 (MQ=255)
                    ggCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTgc                                                                                                                                <  8:91225/147‑1 (MQ=255)
                                  cTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTgg                                                                                                                 <  3:378110/148‑1 (MQ=255)
                                        tACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGt                                                                                                          >  8:229195/1‑149 (MQ=255)
                                          cATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTg                                                                                                         <  1:378852/148‑1 (MQ=255)
                                          cATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGc                                                                                                        <  7:229195/149‑1 (MQ=255)
                                           aTCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCa                                                                                                       >  8:310916/1‑149 (MQ=255)
                                                      ccTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATgcctgcct                                                                                              >  2:213606/1‑147 (MQ=255)
                                                      ccTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGa                                                                                            <  6:295994/149‑1 (MQ=255)
                                                       cTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAg                                                                                           >  4:257393/1‑149 (MQ=255)
                                                             ttcgAATTGTCGGCTGAAGTGCTGGCGGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGACACTGGAACGGGTGCTTGCGTGCCTGAGCGAccc                                                                                     >  2:286643/4‑149 (MQ=255)
                                                                gAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTAc                                                                                  >  1:307434/1‑149 (MQ=255)
                                                                     gTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTtgt                                                                              <  4:90224/148‑1 (MQ=255)
                                                                      tCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTtgtgt                                                                            >  1:119214/1‑148 (MQ=255)
                                                                             aaGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTgcg                                                                      >  8:155210/1‑148 (MQ=255)
                                                                                 gCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAg                                                                   >  3:308177/1‑147 (MQ=255)
                                                                                 gCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAg                                                                   <  4:308177/147‑1 (MQ=255)
                                                                                  cTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAgtgt                                                                >  1:205228/1‑149 (MQ=255)
                                                                                  cTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAgtgt                                                                >  3:250418/1‑149 (MQ=255)
                                                                                   tGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAgtgt                                                                >  3:147122/1‑148 (MQ=255)
                                                                                    ggCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTtgtg                                                                             >  8:144738/1‑134 (MQ=255)
                                                                                    ggCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTtgtg                                                                             <  7:144738/134‑1 (MQ=255)
                                                                                     gCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGAc                                                             <  5:288038/149‑1 (MQ=255)
                                                                                     gCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGAGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGAc                                                             <  8:366965/149‑1 (MQ=255)
                                                                                          gCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCAAAGAATTACGTGAGCAAGCGGAAACTGGAACGGGTGAATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAAt                                                        >  3:38907/1‑149 (MQ=255)
                                                                                            aGGCTTGCATGAGTTTGCGTTCGCTCTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTg                                                      >  4:301765/1‑149 (MQ=255)
                                                                                                ttGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAAt                                                        >  8:166958/1‑143 (MQ=255)
                                                                                                ttGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAAt                                                        <  7:166958/143‑1 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTATGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTg                                                >  4:338795/1‑148 (MQ=255)
                                                                                                   cATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTgg                                               <  3:257393/149‑1 (MQ=255)
                                                                                                         tttGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACg                                         <  8:321809/149‑1 (MQ=255)
                                                                                                           tGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACgg                                        <  1:213606/148‑1 (MQ=255)
                                                                                                                tCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGt                                                                         >  2:189098/1‑110 (MQ=255)
                                                                                                                tCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGt                                                                         <  1:189098/110‑1 (MQ=255)
                                                                                                                     gTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATc                             >  5:105285/1‑149 (MQ=255)
                                                                                                                      tATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACACTACTTGTGGCGTGAGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTAAtat                            >  5:228450/1‑147 (MQ=255)
                                                                                                                      tATGCGGTGTTCGACCAGCACTTGGGCATCACGACCAAGCATTACGTGCGCCAGCGGAAACTGGACCGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCt                            <  2:348240/149‑1 (MQ=255)
                                                                                                                         gCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTggg                         >  5:92204/1‑149 (MQ=255)
                                                                                                                            ttgttCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCg                      <  7:155210/149‑1 (MQ=255)
                                                                                                                              ggtCGCCCAGCCCTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGTAAACTGGAACGGGTACATGCCTGCCTGATCGACAGGACTTGTGGCGTGAGCAGTGTGACGGAATTGGCCCTGGATTACGGATACCTTCATCTGGGGCgct                    >  4:68416/3‑147 (MQ=255)
                                                                                                                               ttCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGtt                   >  1:169483/1‑149 (MQ=255)
                                                                                                                               ttCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGtt                   >  2:145578/1‑149 (MQ=255)
                                                                                                                                   cccAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGAATGCCTGCCTTAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCACTGGATTACTGTTTCCTTCATCTGGGGCGGTTTTcc               <  1:286643/148‑1 (MQ=255)
                                                                                                                                   aCCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGAGGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc               >  6:179626/1‑149 (MQ=255)
                                                                                                                                    ccAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc               >  5:248056/1‑148 (MQ=255)
                                                                                                                                    ccAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCg              <  7:310916/149‑1 (MQ=255)
                                                                                                                                    ccAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCg              <  5:34984/149‑1 (MQ=255)
                                                                                                                                     cAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCga             <  3:313248/149‑1 (MQ=255)
                                                                                                                                     cAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGATTCCTTCATCTGGGGCGGTTTTcc               >  4:90830/1‑147 (MQ=255)
                                                                                                                                      aGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc               >  5:267427/1‑146 (MQ=255)
                                                                                                                                      aGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgag            <  4:250418/149‑1 (MQ=255)
                                                                                                                                       gCACTTGGGCATCTCGCCGAAGCATTACGTGCTCCAGCGGAAACTGGATCGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCgaga           <  4:38907/149‑1 (MQ=255)
                                                                                                                                       gCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCt                                  <  1:360320/126‑1 (MQ=255)
                                                                                                                                       gCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCt                                  >  2:360320/1‑126 (MQ=255)
                                                                                                                                       gCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTcc               >  2:259550/1‑145 (MQ=255)
                                                                                                                                        tgcTTGGGCGTGATGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGAt          >  1:95425/3‑149 (MQ=255)
                                                                                                                                          cTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTcc                                   <  8:139112/122‑1 (MQ=255)
                                                                                                                                              ggCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATc     <  2:307434/148‑1 (MQ=255)
                                                                                                                                               gCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCTAGATTTATCgg   <  6:60236/149‑1 (MQ=255)
                                                                                                                                               gCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCgg   <  6:248056/149‑1 (MQ=255)
                                                                                                                                                cATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGgc  <  2:205228/149‑1 (MQ=255)
                                                                                                                                                 aTCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCgg   >  1:24067/1‑147 (MQ=255)
                                                                                                                                                 aTCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCgg   >  2:392578/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCTGAGTGCCCCGCAGGCTGGGCTAGAGCGCATTCTTGATTACATCGAACGCAACCTGAAATTGGAATTGTCGGCTGAAGTGCTGGCAGAGCAGGCTTGCATGAGTTTGCGTTCGCTGTATGCGTTGTTCGACCAGCACTTGGGCATCACGCCCAAGCATTACGTGCGCCAGCGGAAACTGGAACGGGTGCATGCCTGCCTGAGCGACCCTACTTGTGGCGTGCGCAGTGTGACGGAATTGGCCCTGGATTACGGTTTCCTTCATCTGGGGCGGTTTTCCGAGATTTATCGGC  >  NC_002947/3580483‑3580775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑