Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,624,144 G→C 7.4% intergenic (‑92/+81) PP_3194 ← / ← PP_3195 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,624,1440GC 7.4% 151.7 / 2.2 53intergenic (‑92/+81)PP_3194/PP_3195hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base C (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTGCAGGCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATGC  >  NC_002947/3623997‑3624289
                                                                                                                                                   |                                                                                                                                                 
gtgtGCAGGCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATgg                                                                                                                                                   >  2:380182/1‑148 (MQ=255)
   tgCAGGCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCAt                                                                                                                                                <  3:258702/148‑1 (MQ=255)
      aGGCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGa                                                                                                                                             >  8:46239/1‑148 (MQ=255)
         ccAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATAcc                                                                                                                                         <  1:380182/149‑1 (MQ=255)
           aGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTg                                                                                                                                       <  5:97424/149‑1 (MQ=255)
           aGTGCCGTAGAACATGGGGGAGCAGTGCATGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGTCTAGGCTGTGGCGACTGCCCTGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTg                                                                                                                                       <  8:302982/149‑1 (MQ=255)
                cGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGCCATCGATAc                                                                                                                                          <  4:15258/141‑1 (MQ=255)
                cGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGCCATCGATAc                                                                                                                                          >  3:15258/1‑141 (MQ=255)
                          ggTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGgcg                                                                                                                          >  5:231503/1‑147 (MQ=255)
                          ggTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGAAGGCATCGATACATGGCACTCTCTCgcgc                                                                                                                         >  8:256931/1‑148 (MQ=255)
                                  aGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACgag                                                                                                                <  7:256931/149‑1 (MQ=255)
                                   gtgcaggtgcagGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACgag                                                                                                                >  1:354675/1‑148 (MQ=255)
                                         gtgcaggGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgcg                                                                                                         >  5:317634/1‑149 (MQ=255)
                                                  aCTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGgc                                                                                                <  1:13770/149‑1 (MQ=255)
                                                    tCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGgcgc                                                                                              >  5:257216/1‑149 (MQ=255)
                                                      aTTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGgcgc                                                                                              <  6:257216/147‑1 (MQ=255)
                                                      aTTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGcgccg                                                                                            >  5:162060/1‑149 (MQ=255)
                                                       ttGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGcgccgc                                                                                           >  2:383925/1‑149 (MQ=255)
                                                           tGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCaa                                                                                       >  5:125401/1‑149 (MQ=255)
                                                             aGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCGATTGGcgccgttctcg                                                                                      <  7:46239/148‑7 (MQ=255)
                                                                  tctcATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAggg                                                                                >  7:336503/1‑149 (MQ=255)
                                                                  tctcATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAg                                                                                  <  3:26484/147‑1 (MQ=255)
                                                                  tctcATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAg                                                                                  >  4:26484/1‑147 (MQ=255)
                                                                    tcttCTTGGGGGTGGTGAAAGGTTAGGCTGTGGCGACTGCCCGGCACCATCATATGCTATTGATTGCCTGGTATCGATTGCATCGATACCTGGCTCTCTCTGTCGCAGAAACGTGAACGGCGCCAATCGGTGCCGCCCAAGCCAggg                                                                                <  4:88586/144‑1 (MQ=255)
                                                                    tcATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGcc                                                                              >  3:200771/1‑149 (MQ=255)
                                                                      aTCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCgg                                                                            >  4:304761/1‑149 (MQ=255)
                                                                           ggggggggggCATGGCTAGGCTGTGGCGACTGCCCGGCACCAGCATATGCTTTTGATGGCCAGGTATCGTTGGCAGCGATACCTGGCGCTCTCTGGCGCAGAAACGACAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCgg                                                                            <  4:22938/144‑1 (MQ=255)
                                                                           gggggTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCgg                                                                            >  3:22938/1‑144 (MQ=255)
                                                                                tggtgCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGAt                                                                  <  6:125401/149‑1 (MQ=255)
                                                                                tggtgCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGAt                                                                  <  7:335558/149‑1 (MQ=255)
                                                                                    gCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATAGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAgg                                                              >  1:116513/1‑149 (MQ=255)
                                                                                     catggagagggtGCGGCGACGCCCCGGCACCTGCAGATGCTTGTGATGCCCTTGTATCGTTGGCAGCGATACCTGGCGCTCTCTGGCGCAGACCCGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAgg                                                              <  6:231503/141‑1 (MQ=255)
                                                                                      aaGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGcc                                                            <  6:317634/149‑1 (MQ=255)
                                                                                             ggctGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGgc                                                     >  4:87348/1‑149 (MQ=255)
                                                                                                         tGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGttt                                         >  5:141206/1‑149 (MQ=255)
                                                                                                           cccGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTCTGGCCGCCAAGGGTTTCg                                       >  3:11876/1‑149 (MQ=255)
                                                                                                            ccGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCgg                                      >  2:263574/1‑149 (MQ=255)
                                                                                                               gCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGa                                   <  3:156566/149‑1 (MQ=255)
                                                                                                                 aCCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATc                                 <  1:383925/149‑1 (MQ=255)
                                                                                                                  ccAGCAGATGCTATTGATGGCCAGTTATCGATGCCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATcc                                <  7:53176/149‑1 (MQ=255)
                                                                                                                    agcagATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAg                              >  2:80219/1‑149 (MQ=255)
                                                                                                                         aTGCTATTGCTGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGATACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGc                         >  4:268939/1‑149 (MQ=255)
                                                                                                                           gCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGa                       >  7:350322/1‑149 (MQ=255)
                                                                                                                               ttGATGGCCAGGTATCGATAGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATg                     <  2:116513/147‑1 (MQ=255)
                                                                                                                                      ccAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCg            <  8:336503/149‑1 (MQ=255)
                                                                                                                                       cAGGTATCGATGCCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCgg           >  5:288399/1‑149 (MQ=255)
                                                                                                                                        aGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCgg           >  5:81127/1‑148 (MQ=255)
                                                                                                                                          gTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGACGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCAt        >  4:249193/1‑149 (MQ=255)
                                                                                                                                          gTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCCGCAGCGCCGATCAGGCCGAGTGGCCGCCAAGGGTTTCGGCGATCCAGGAGGCGCTGTAGTGCCCGGCAt        >  7:153690/1‑149 (MQ=255)
                                                                                                                                             tCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGa                       >  4:224735/1‑131 (MQ=255)
                                                                                                                                             tCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGa                       <  3:224735/131‑1 (MQ=255)
                                                                                                                                               gATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGAt                                  <  2:295499/118‑1 (MQ=255)
                                                                                                                                               gATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGAt                                  >  1:295499/1‑118 (MQ=255)
                                                                                                                                                aTGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATGc  <  8:350322/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTGTGCAGGCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATGC  >  NC_002947/3623997‑3624289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑