Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,637,154 A→G 7.3% H361R (CAC→CGC)  PP_3203 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,637,1540AG 7.3% 155.8 / 2.9 55H361R (CAC→CGC) PP_3203MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (25/26);  new base G (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

TGCTGCCGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCT  >  NC_002947/3637007‑3637302
                                                                                                                                                   |                                                                                                                                                    
tgctgcCGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCAc                                                                                                                                                     >  2:50070/1‑149 (MQ=255)
   tgcCGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTg                                                                                                                                                  >  4:356631/1‑149 (MQ=255)
     ccGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTg                                                                                                                                                  >  4:3544/1‑147 (MQ=255)
     ccGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGc                                                                                                                                                 <  1:190638/148‑1 (MQ=255)
      cGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCg                                                                                                                                               >  6:25113/1‑149 (MQ=255)
                cACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCGCCTGCCGCATGCActgc                                                                                                                                     >  2:373197/1‑149 (MQ=255)
                cACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCGCCTGCCGCATGCActgc                                                                                                                                     >  2:367375/1‑149 (MQ=255)
                 aCCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCActgc                                                                                                                                     <  8:264490/148‑1 (MQ=255)
                  cccTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCa                                                                                                                                         >  1:312613/1‑143 (MQ=255)
                  cccTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCa                                                                                                                                         <  2:312613/143‑1 (MQ=255)
                        tGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTc                                                                                                                             <  6:154284/149‑1 (MQ=255)
                               gcagGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCg                                                                                                                      >  5:17319/1‑149 (MQ=255)
                               gcagGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCg                                                                                                                      <  5:25113/149‑1 (MQ=255)
                                 agGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGa                                                                                                                    >  2:132724/1‑149 (MQ=255)
                                    ttGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACaa                                                                                                                 <  3:356631/149‑1 (MQ=255)
                                           cGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgct                                                                                                            <  5:270169/147‑1 (MQ=255)
                                            gATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCGCCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgct                                                                                                         <  1:373197/149‑1 (MQ=255)
                                            gATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCGCCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgct                                                                                                         <  1:367375/149‑1 (MQ=255)
                                             aTCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgctg                                                                                                        >  5:245966/1‑149 (MQ=255)
                                             aTCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgctg                                                                                                        >  7:327267/1‑149 (MQ=255)
                                                 gcgcGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGgcgc                                                                                                    >  6:71651/1‑149 (MQ=255)
                                                 gcgcGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGgcgc                                                                                                    >  8:16032/1‑149 (MQ=255)
                                                 gcgcGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGgcgc                                                                                                    <  4:143396/149‑1 (MQ=255)
                                                     ggCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCtgt                                                                                                <  8:327267/149‑1 (MQ=255)
                                                      gCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCtgtg                                                                                               >  2:172152/1‑149 (MQ=255)
                                                         gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                            >  8:304056/1‑149 (MQ=255)
                                                         gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                            <  1:50070/149‑1 (MQ=255)
                                                         gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                            <  1:62769/149‑1 (MQ=255)
                                                          tGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGcc                                                                                           >  3:135339/1‑149 (MQ=255)
                                                            ccACGGTTGGGTTCACTTCGGGGATTTTCCGGGGTGAGTTCGGCAGTCCATTGATCGTCCTGGCATTGACCGCTGTTTTGGCTACGCACCTGCCGCATGCACTGCTGCTTGTCGCCATctg                                                                                                                     <  2:366524/121‑3 (MQ=255)
                                                            ccACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTg                                                                                         >  4:102421/1‑149 (MQ=255)
                                                            ccACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTAGTCGCCATCgg                                                                                                                     >  1:366524/1‑121 (MQ=255)
                                                              aCGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTc                                                                                       >  2:185635/1‑149 (MQ=255)
                                                                         cACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATgcgc                                                                             >  2:339524/1‑148 (MQ=255)
                                                                          aCTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCa                                                                           >  2:328475/1‑149 (MQ=255)
                                                                                  gATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCCTTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTg                                                                   <  1:132724/149‑1 (MQ=255)
                                                                                     tttCGAGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTTCCGCTTGCACTTCTGCTGGTCGCCATCGTACAACTGCTGCTTGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCg                                                                 <  8:252458/148‑1 (MQ=255)
                                                                                              tGAGGTCGGCAGTCCCTTGATCGTCCTGGCATGGTCCGCTGGGGTGGCTACGCACCTGCCGCATGCTCTGCTGCTGGTCGGCATCGGACATCTTCTGCTGGCGCCTGTTTGCCTGTCCTTGATGCGCCACCGGCGGGCGGTGACGgggg                                                       <  4:141587/149‑1 (MQ=255)
                                                                                                  ttagTCAGTCCATTGATCTTCCTGGCTTTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAgg                                                   <  6:17319/146‑1 (MQ=255)
                                                                                                      tCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCa                                               <  6:245966/149‑1 (MQ=255)
                                                                                                           ccATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGAt                                           <  4:135339/148‑1 (MQ=255)
                                                                                                             aTTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGaa                                        >  6:244186/1‑149 (MQ=255)
                                                                                                                 aTCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGa                                     >  5:389109/1‑148 (MQ=255)
                                                                                                                    gTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAAtt                                 <  5:71651/149‑1 (MQ=255)
                                                                                                                       cTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTc                              <  5:285371/149‑1 (MQ=255)
                                                                                                                          gCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCt                            <  6:389109/148‑1 (MQ=255)
                                                                                                                                aCCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTggcggg                     <  1:339524/149‑1 (MQ=255)
                                                                                                                                  cGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTggcgggc                    >  6:195288/1‑148 (MQ=255)
                                                                                                                                   gCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGa                  <  3:102421/149‑1 (MQ=255)
                                                                                                                                      ggTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGc                >  5:124942/1‑148 (MQ=255)
                                                                                                                                       gtgtGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCTTTTGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGc                <  1:328475/147‑1 (MQ=255)
                                                                                                                                          tgGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAAt            <  7:16032/148‑1 (MQ=255)
                                                                                                                                            gCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCgg                                                                >  1:372884/1‑94 (MQ=255)
                                                                                                                                            gCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCgg                                                                <  2:372884/94‑1 (MQ=255)
                                                                                                                                            gCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGc          >  6:371471/1‑148 (MQ=255)
                                                                                                                                                   aCCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGct  >  3:99399/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCTGCCGACGCTGATCACCCTGGTGATGCAGCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCT  >  NC_002947/3637007‑3637302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑