Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,701,987	G→A	6.7%	L139L (CTG→CTA) ‡	PP_3267 →	Clp protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,701,987	0	G	A	6.7%	219.0 / 3.8	74	L139L (CTG→CTA) ‡	PP_3267	Clp protease
Reads supporting (aligned to +/- strand):  ref base G (37/32);  new base A (2/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCG  >  NC_002947/3701848‑3702132                                                                                                                                               |                                                                                                                                                   tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAg                                                                                                                                           >  4:296091/1‑149 (MQ=255) tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                              >  2:71419/1‑146 (MQ=255)  ctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                              >  7:364705/1‑145 (MQ=255)     ccTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCATACAGGCGCAGGTc                                                                                                                                        <  6:249704/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGATAGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATc                                                                                                                                        <  1:70103/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCg                                                                                                                                       <  4:64517/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGAGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGt                                                                                                                                      >  6:39265/1‑149 (MQ=255)         gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCGtaa                                                                                                                                    <  3:296091/148‑3 (MQ=255)           cGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑g                                                                                                                                 >  6:275979/1‑148 (MQ=255)                    aCGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACg                                                                                                                        >  8:16274/1‑148 (MQ=255)                      gCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGa                                                                                                                       >  8:45134/1‑147 (MQ=255)                       cACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAg                                                                                                                    >  1:8923/1‑149 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                             <  1:17785/124‑1 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                             >  2:17785/1‑124 (MQ=255)                         cGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGgc                                                                                                                  <  8:364705/149‑1 (MQ=255)                                 aGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGa                                                                                                          <  4:236064/149‑1 (MQ=255)                                        gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCATGAAAGGCGCGGCCGAGGTTTTg                                                                                                   <  3:84104/149‑1 (MQ=255)                                        gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                   >  6:167487/1‑149 (MQ=255)                                         cAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                   >  8:57104/1‑148 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                 >  6:147270/1‑149 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                 <  7:145758/149‑1 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                 <  7:45134/149‑1 (MQ=255)                                            gTTTCTTTTCCGCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                               >  4:265875/1‑149 (MQ=255)                                            gTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTGACCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                 >  1:112083/1‑149 (MQ=255)                                            gTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                               >  7:143113/1‑149 (MQ=255)                                                     ccACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGt                                                                                       >  8:329601/1‑148 (MQ=255)                                                       aCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTAt                                                                                    <  7:57104/149‑1 (MQ=255)                                                           gTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTaa                                                                                >  2:116935/1‑149 (MQ=255)                                                            tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAAt                                                                               <  1:302035/149‑1 (MQ=255)                                                            tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCCAAt                                                                               <  5:39265/149‑1 (MQ=255)                                                              tCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATg                                                                              <  7:16274/148‑1 (MQ=255)                                                               cATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGTGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             <  1:268574/148‑1 (MQ=255)                                                               cATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             <  8:255044/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGcc                                                                           >  7:350907/1‑149 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                            <  5:275979/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                            <  2:112083/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                            <  7:329601/148‑1 (MQ=255)                                                                    gACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATc                                                                       >  7:16705/1‑149 (MQ=255)                                                                       cTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATcc                                                                      >  1:146579/1‑147 (MQ=255)                                                                       cTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCaa                                                                    <  5:259278/149‑1 (MQ=255)                                                                            aTGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCTCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATa                                                               <  7:177696/149‑1 (MQ=255)                                                                                ttCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTgg                                                           <  1:356883/149‑1 (MQ=255)                                                                                    gTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgcc                                                       >  7:311261/1‑149 (MQ=255)                                                                                         ccATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             <  6:46003/122‑1 (MQ=255)                                                                                         ccATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             >  5:46003/1‑122 (MQ=255)                                                                                           aTTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgcctcccgcg                                                >  6:355705/1‑142 (MQ=255)                                                                                            ttCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAgg                                               <  7:277752/149‑1 (MQ=255)                                                                                                 ggATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                          <  8:143113/149‑1 (MQ=255)                                                                                                 ggATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                          >  7:93480/1‑149 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             >  6:105245/1‑107 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                             <  5:105245/107‑1 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                   <  3:270137/149‑1 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                  >  4:160629/1‑149 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                  <  2:379220/149‑1 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                  <  1:71419/149‑1 (MQ=255)                                                                                                            aGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                  >  3:248296/1‑148 (MQ=255)                                                                                                                   gAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATcc                          <  2:8923/149‑1 (MQ=255)                                                                                                                   gAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATc                           >  8:11582/1‑148 (MQ=255)                                                                                                                     t‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCAGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCa                         >  1:74346/1‑149 (MQ=255)                                                                                                                        aGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATg                       <  7:11582/148‑1 (MQ=255)                                                                                                                          ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGaaa                    >  6:157739/1‑149 (MQ=255)                                                                                                                           cTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAAt                   >  7:141382/1‑149 (MQ=255)                                                                                                                            tGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATc                  >  3:272697/1‑149 (MQ=255)                                                                                                                               aTTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac               <  8:141382/149‑1 (MQ=255)                                                                                                                                ttACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac               >  1:29540/1‑148 (MQ=255)                                                                                                                                 tACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga                <  6:56358/146‑1 (MQ=255)                                                                                                                                 tACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga                >  5:56358/1‑146 (MQ=255)                                                                                                                                 caCGATCTGCGCATACAGGCGCAGGTCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac               >  6:25048/2‑147 (MQ=255)                                                                                                                                    gACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGAt          <  8:350907/149‑1 (MQ=255)                                                                                                                                     aCCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATg         >  6:288482/1‑149 (MQ=255)                                                                                                                                       cTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGACCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGcc       >  1:81783/1‑149 (MQ=255)                                                                                                                                        tGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                <  8:316318/123‑1 (MQ=255)                                                                                                                                        tGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                >  7:316318/1‑123 (MQ=255)                                                                                                                                            ccTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATcg  >  2:312907/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                   TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCG  >  NC_002947/3701848‑3702132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑