Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,701,990	T→G	6.4%	Y140* (TAT→TAG) ‡	PP_3267 →	Clp protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,701,990	0	T	G	6.4%	245.2 / 6.2	78	Y140* (TAT→TAG) ‡	PP_3267	Clp protease
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base G (2/3);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTC  >  NC_002947/3701848‑3702136                                                                                                                                                  |                                                                                                                                                    tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAg                                                                                                                                               >  4:296091/1‑149 (MQ=255) tctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                                  >  2:71419/1‑146 (MQ=255)  ctTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcg                                                                                                                                                  >  7:364705/1‑145 (MQ=255)     ccTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCATACAGGCGCAGGTc                                                                                                                                            <  6:249704/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGATAGAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATc                                                                                                                                            <  1:70103/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCg                                                                                                                                           <  4:64517/148‑1 (MQ=255)      cTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGAGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGt                                                                                                                                          >  6:39265/1‑149 (MQ=255)         gCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGATCTGCGCATACAGGCGCAGGTCGtaa                                                                                                                                        <  3:296091/148‑3 (MQ=255)           cGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑g                                                                                                                                     >  6:275979/1‑148 (MQ=255)                    aCGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACg                                                                                                                            >  8:16274/1‑148 (MQ=255)                      gCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGa                                                                                                                           >  8:45134/1‑147 (MQ=255)                       cACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAg                                                                                                                        >  1:8923/1‑149 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                                 <  1:17785/124‑1 (MQ=255)                        aCGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATgcgc                                                                                                                                                 >  2:17785/1‑124 (MQ=255)                         cGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGgc                                                                                                                      <  8:364705/149‑1 (MQ=255)                                 aGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGa                                                                                                              <  4:236064/149‑1 (MQ=255)                                        gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCATGAAAGGCGCGGCCGAGGTTTTg                                                                                                       <  3:84104/149‑1 (MQ=255)                                        gCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                       >  6:167487/1‑149 (MQ=255)                                         cAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTg                                                                                                       >  8:57104/1‑148 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                     >  6:147270/1‑149 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                     <  7:145758/149‑1 (MQ=255)                                          aaGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                     <  7:45134/149‑1 (MQ=255)                                            gTTTCTTTTCCGCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                                   >  4:265875/1‑149 (MQ=255)                                            gTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGTGACCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGa                                                                                                     >  1:112083/1‑149 (MQ=255)                                            gTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATg                                                                                                   >  7:143113/1‑149 (MQ=255)                                                     ccACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGt                                                                                           >  8:329601/1‑148 (MQ=255)                                                       aCCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTAt                                                                                        <  7:57104/149‑1 (MQ=255)                                                           gTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTaa                                                                                    >  2:116935/1‑149 (MQ=255)                                                            tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAAt                                                                                   <  1:302035/149‑1 (MQ=255)                                                            tttCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCCAAt                                                                                   <  5:39265/149‑1 (MQ=255)                                                              tCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATg                                                                                  <  7:16274/148‑1 (MQ=255)                                                               cATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGTGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 <  1:268574/148‑1 (MQ=255)                                                               cATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 <  8:255044/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGcc                                                                               >  7:350907/1‑149 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                                <  2:112083/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                                <  5:275979/148‑1 (MQ=255)                                                                aTTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGc                                                                                <  7:329601/148‑1 (MQ=255)                                                                    gACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATc                                                                           >  7:16705/1‑149 (MQ=255)                                                                       cTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATcc                                                                          >  1:146579/1‑147 (MQ=255)                                                                       cTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCaa                                                                        <  5:259278/149‑1 (MQ=255)                                                                            aTGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCTCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATa                                                                   <  7:177696/149‑1 (MQ=255)                                                                                ttCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTgg                                                               <  1:356883/149‑1 (MQ=255)                                                                                    gTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgcc                                                           >  7:311261/1‑149 (MQ=255)                                                                                         ccATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 >  5:46003/1‑122 (MQ=255)                                                                                         ccATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 <  6:46003/122‑1 (MQ=255)                                                                                           aTTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGcgcctcccgcg                                                    >  6:355705/1‑142 (MQ=255)                                                                                            ttCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAgg                                                   <  7:277752/149‑1 (MQ=255)                                                                                                 ggATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                              >  7:93480/1‑149 (MQ=255)                                                                                                 ggATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTg                                              <  8:143113/149‑1 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 >  6:105245/1‑107 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATgg                                                                                 <  5:105245/107‑1 (MQ=255)                                                                                                          cGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACg                                       <  3:270137/149‑1 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                      <  2:379220/149‑1 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                      >  4:160629/1‑149 (MQ=255)                                                                                                           gAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                      <  1:71419/149‑1 (MQ=255)                                                                                                            aGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACgg                                      >  3:248296/1‑148 (MQ=255)                                                                                                                   gAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATcc                              <  2:8923/149‑1 (MQ=255)                                                                                                                   gAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATc                               >  8:11582/1‑148 (MQ=255)                                                                                                                     t‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGCAGGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCa                             >  1:74346/1‑149 (MQ=255)                                                                                                                        aGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATg                           <  7:11582/148‑1 (MQ=255)                                                                                                                          ccTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGaaa                        >  6:157739/1‑149 (MQ=255)                                                                                                                           cTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAAt                       >  7:141382/1‑149 (MQ=255)                                                                                                                            tGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATc                      >  3:272697/1‑149 (MQ=255)                                                                                                                               aTTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   <  8:141382/149‑1 (MQ=255)                                                                                                                                ttACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  1:29540/1‑148 (MQ=255)                                                                                                                                 tACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga                    <  6:56358/146‑1 (MQ=255)                                                                                                                                 tACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcga                    >  5:56358/1‑146 (MQ=255)                                                                                                                                 caCGATCTGCGCATACAGGCGCAGGTCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATcgac                   >  6:25048/2‑147 (MQ=255)                                                                                                                                    gACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGAt              <  8:350907/149‑1 (MQ=255)                                                                                                                                     aCCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATg             >  6:288482/1‑149 (MQ=255)                                                                                                                                       cTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGACCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGcc           >  1:81783/1‑149 (MQ=255)                                                                                                                                        tGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                    >  7:316318/1‑123 (MQ=255)                                                                                                                                        tGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGga                                    <  8:316318/123‑1 (MQ=255)                                                                                                                                            ccTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATcg      >  2:312907/1‑149 (MQ=255)                                                                                                                                                tATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGTCTATCCAAGGATACTGGCGCCGCGGGAGGCGCGGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGt   <  1:70070/148‑1 (MQ=255)                                                                                                                                                 aTGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGt   >  3:171439/1‑147 (MQ=255)                                                                                                                                                 aTGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTc  <  3:160629/148‑1 (MQ=255)                                                                                                                                                 aTGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTc  <  4:248296/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCTTCCTTGCCGGAGAGCGACGCACGGCTTGCAGTTTCAGCAAGTTTCTTTTCCACCCGTTTCATTGGACCTTGCATGGTTCGGTGGACCATTCGCGGATGT‑‑CCGAGTATGCGAT‑GAGCCTGGATTACGACCTGCGCCTGTATGCGCAGATCGTGGC‑GGAGCGCACGAAAGGCGCGGCCGAGGTTTTGGATGTACCGCGTTATCTAATGGCCTATCCAAGGATACTGGCGCCGCGGGAGGCGCTGGAAAACGGGATGATCCATGAAATCGACGAGATGCCGATCGCGTC  >  NC_002947/3701848‑3702136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑