| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,730,931 | G→C | 7.0% | intergenic (+226/‑172) | PP_3297 → / → PP_3298 | hypothetical protein/CinA domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,730,931 | 0 | G | C | 7.0% | 169.9 / 4.3 | 57 | intergenic (+226/‑172) | PP_3297/PP_3298 | hypothetical protein/CinA domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (28/24); new base C (2/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01 | |||||||||||
ATAATGCATATTATGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGACACGTCCACC > NC_002947/3730788‑3731075 | aTAATGCATATTATTTTAAATATAGTGCGATGTAATAGATTTTCGTGCGCTACCTATGATGCAGATTCGACAGCCCCGTTTTGGCATCCACGCTTTCAACTGTCCAGGTTATGGGTGTTCAACGCGACCCAACACATTCAGCTGGcgt > 8:183900/1‑146 (MQ=255) gCATATTATGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAAt > 1:253265/1‑148 (MQ=255) atatTATGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCACGTTTTGGAATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACACCAAACATTGAGGTGGCTGCCTATGAt > 5:169849/1‑149 (MQ=255) attatGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACA‑‑GACCCCAAACATTGAGGTGGCTGCCAATGATCCgg > 4:34306/1‑149 (MQ=255) ttatGTTAAATATCGTGCTATGTCATAGATGTTCGGGCGCTACCTATGCTGCAGATTCGACAGCCCATGTTTGGCATCCACTCTTTCAACGGTCCATGTGCTGTGGGTTCACAGCGCCCCCAAACATTGAGGTTGCTGCCAATGCTcct < 7:183900/149‑2 (MQ=255) atGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGc > 1:194391/1‑149 (MQ=255) gTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCt > 6:231138/1‑149 (MQ=255) ttAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCt > 4:199465/1‑148 (MQ=255) gTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTccc < 2:36602/149‑1 (MQ=255) cTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCccgcc < 2:370791/149‑1 (MQ=255) aggTCATAGATGTTCGTGCGCTACCTAGGCGTCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGc < 6:380659/130‑1 (MQ=255) tCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTccc > 4:148666/1‑141 (MQ=255) tCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTccc < 3:148666/141‑1 (MQ=255) tCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGt > 2:52520/1‑149 (MQ=255) tCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGt < 7:146634/149‑1 (MQ=255) aTAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATGCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTc < 3:202692/148‑1 (MQ=255) gcTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGt > 2:352097/1‑149 (MQ=255) cTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTg < 2:253265/149‑1 (MQ=255) ccTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGa < 4:187393/147‑1 (MQ=255) ccTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATACGAATAGAAGATTACCGCCGATGTCACTGTGCTCGGCAGTGATg > 1:329376/1‑149 (MQ=255) ccTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACA‑‑GACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATg < 3:34306/147‑1 (MQ=255) cTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTAc < 1:219388/149‑1 (MQ=255) cTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTAc < 5:231138/149‑1 (MQ=255) aGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGGGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAgg < 7:347962/149‑1 (MQ=255) aGATTCGACAGCCCCGTGTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAgg < 6:169849/149‑1 (MQ=255) aGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCATACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAg > 1:82721/1‑149 (MQ=255) cccGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGtt > 2:117717/1‑148 (MQ=255) ccGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGTCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCa < 7:312930/149‑1 (MQ=255) cGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAg < 3:199465/149‑1 (MQ=255) aTCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGaaaa > 6:291929/1‑147 (MQ=255) ccACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGaaaa > 3:293295/1‑145 (MQ=255) ccACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGaaaa < 4:293295/145‑1 (MQ=255) ccACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAg > 8:34983/1‑148 (MQ=255) tctTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTCGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGtt > 4:344997/1‑131 (MQ=255) tctTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTCGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGtt > 4:350698/1‑131 (MQ=255) tctTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTCGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGtt < 3:344997/131‑1 (MQ=255) tctTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTCGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGtt < 3:350698/131‑1 (MQ=255) cAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGc > 4:109402/1‑149 (MQ=255) aaCGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCt > 4:271702/1‑149 (MQ=255) cGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑gc < 5:291929/149‑1 (MQ=255) ccATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCt > 5:372109/1‑148 (MQ=255) tGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGt > 6:136745/1‑148 (MQ=255) ggTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTg < 2:194391/147‑1 (MQ=255) ggTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGaa < 1:352097/149‑1 (MQ=255) gtTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGaa > 2:22871/1‑121 (MQ=255) gtTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGaa < 1:22871/121‑1 (MQ=255) acaGCGACCCCAAACATTGAGGTGGCTGCCAATGCTCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTa > 7:280873/1‑149 (MQ=255) acaGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTa > 1:113272/1‑149 (MQ=255) acaGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTa > 3:376351/1‑149 (MQ=255) acaGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGAGAACCAttt < 2:329376/148‑1 (MQ=255) ccccAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGcc < 7:34983/149‑1 (MQ=255) cccAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGCAAACAGAAGGCGAAGAT‑GCGCTAACCCGGTGACCCATTTACATAGcc > 6:20629/1‑148 (MQ=255) cAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGa > 1:65541/1‑148 (MQ=255) cAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGa > 5:335396/1‑148 (MQ=255) aaCATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCTCGCGCTAAACCGGTGAACCATTTACATAGCCGaca < 1:52520/149‑1 (MQ=255) ttGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGACACGTc > 4:222798/1‑148 (MQ=255) aGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGACACGTCCAcc < 6:372109/149‑1 (MQ=255) | ATAATGCATATTATGTTAAATATCGTGCTATGTCATAGATGTTCGTGCGCTACCTAGGCTGCAGATTCGACAGCCCCGTTTTGGCATCCACTCTTTCAACGGTCCATGTTCTGGGTGTTCACAGCGACCCCAAACATTGAGGTGGCTGCCAATGATCCGGCCTGAAGATTCCCGCCGATGTCACTGTGCTCGGCAGTGATGTTCTACGAGGGAAATAAAGTTCAGTTGAAAACAGAAGGCGAAGCT‑GCGCTAAACCGGTGAACCATTTACATAGCCGACACGTCCACC > NC_002947/3730788‑3731075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |