Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,785,051 C→T 8.5% R82R (CGC→CGT)  PP_3348 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,785,0510CT 8.5% 197.8 / 6.2 71R82R (CGC→CGT) PP_3348GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base T (4/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01

TTCCTGGCCGCCGTCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCATGTCTGTCAGC  >  NC_002947/3784905‑3785198
                                                                                                                                                  |                                                                                                                                                   
ttCCTGGCCGCCGTCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCAc                                                                                                                                                   >  2:52032/1‑149 (MQ=255)
     ggCCGCCGTCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGcc                                                                                                                                               >  4:10939/1‑148 (MQ=255)
     ggCCGCCGTCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGcc                                                                                                                                               >  7:370532/1‑148 (MQ=255)
           cgTGATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGGCATGGTCTGGATGCCGTTATTGGGCGTGTCGACCGGCGTGCGTACGGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCATCCAGCGCGCGGCGGAgcgcg                                                                                                                                        >  6:329414/1‑148 (MQ=255)
             tCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCTCCCGCCAACCAGCGCACGGCCGAACGCTgg                                                                                                                                      <  1:33347/149‑1 (MQ=255)
             tCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTgg                                                                                                                                      <  5:10443/149‑1 (MQ=255)
             tCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTgg                                                                                                                                      <  6:287655/149‑1 (MQ=255)
             tCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTgg                                                                                                                                      <  5:224477/149‑1 (MQ=255)
             tCACGCAGTGCGGGCTCGGCTGCGAGCGTTTCGACCCGCAAACGGTCATGGTCGGGATGACGTAATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCggg                                                                                                                                      <  8:6696/149‑1 (MQ=255)
               aTGCTGTGCTGGTTGGGCGGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGa                                                                                                                                    <  3:289893/149‑1 (MQ=255)
                tgctgtgctgGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGTTCGGCCGTGCGCTggttg                                                                                                                                   <  3:144907/149‑4 (MQ=255)
                tgctgtgctgGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAg                                                                                                                                   <  4:348493/149‑1 (MQ=255)
                  ctgtgctgGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGTTCGGCCGTGCGCTggttggc                                                                                                                                 <  3:256533/149‑6 (MQ=255)
                  ctgtgctgGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGt                                                                                                                                  <  8:370532/148‑1 (MQ=255)
                     tgctgGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCg                                                                                                                              <  5:135103/149‑1 (MQ=255)
                           ttGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTg                                                                                                                        <  1:236043/149‑1 (MQ=255)
                             gggCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTgg                                                                                                                       <  3:10939/148‑1 (MQ=255)
                                        gTTTCGAGCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGGGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGGCTATCTGCGTGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATg                                                                                                           <  2:67166/149‑1 (MQ=255)
                                        gTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATg                                                                                                           <  6:376938/149‑1 (MQ=255)
                                        gTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATg                                                                                                           >  4:41302/1‑149 (MQ=255)
                                         tttCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGc                                                                                                          >  5:139885/1‑149 (MQ=255)
                                                 cGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTcggcg                                                                                                  <  7:366038/149‑1 (MQ=255)
                                                   cAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCggcggc                                                                                                >  8:307891/1‑149 (MQ=255)
                                                          tCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctgg                                                                                         <  7:213962/149‑1 (MQ=255)
                                                          tCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctgg                                                                                         <  5:333349/149‑1 (MQ=255)
                                                            aTGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctgggg                                                                                       >  1:248223/1‑149 (MQ=255)
                                                            aTGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctggg                                                                                        <  1:170302/148‑1 (MQ=255)
                                                             tGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctggggc                                                                                      >  3:204543/1‑149 (MQ=255)
                                                                          gTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTg                                                                         <  2:248223/149‑1 (MQ=255)
                                                                          gTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCAAGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGCAGCTCTCGCGGGCTATCTGCGGCTGGGGCGCCTTTg                                                                         >  5:129204/1‑149 (MQ=255)
                                                                           ttATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCttt                                                                          <  5:384148/147‑1 (MQ=255)
                                                                           ttATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCttt                                                                          >  6:384148/1‑147 (MQ=255)
                                                                           ttATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGc                                                                        <  3:41302/149‑1 (MQ=255)
                                                                            tATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGTTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGcc                                                                       >  4:148735/1‑149 (MQ=255)
                                                                             aTTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCg                                                                      >  2:78989/1‑149 (MQ=255)
                                                                              ttGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCg                                                                      <  6:139885/148‑1 (MQ=255)
                                                                               tGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGt                                                                     >  8:62053/1‑148 (MQ=255)
                                                                                gggCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctggggctggg                                                                                  <  2:93610/134‑1 (MQ=255)
                                                                                gggCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATctggggctggg                                                                                  >  1:93610/1‑134 (MQ=255)
                                                                                 ggCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTaa                                                                   <  2:312230/148‑1 (MQ=255)
                                                                                    gggtCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGcc                                                               <  1:203541/147‑1 (MQ=255)
                                                                                         gACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCg                                                             >  5:284405/1‑146 (MQ=255)
                                                                                           ccGGAGTGCGTACCGCCATGTTCTTAGCCTTTCTGGGAGCACCTGTTAACCAGTGCTCTGCCGAAGGCTGGGAGTGGGGTTACTGGGTGTGGCTGATGCTGTCTGCGGCTATCTGGGGCTGGGGCTCCTTTGCCGTAATGCCCGCAga                                                         <  4:204543/148‑1 (MQ=255)
                                                                                               cgtgcgtACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGacgacg                                                    <  7:62053/149‑1 (MQ=255)
                                                                                                     tACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGAGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTgg                                              >  8:238864/1‑149 (MQ=255)
                                                                                                        cGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGc                                           <  3:120307/149‑1 (MQ=255)
                                                                                                         gCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTAGCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCa                                          <  7:307891/149‑1 (MQ=255)
                                                                                                           cATGTTCTTCGCCTATCTGCGAGCACCCCCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGa                                        >  1:219462/1‑149 (MQ=255)
                                                                                                             tGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACg                                      >  5:115186/1‑149 (MQ=255)
                                                                                                                 cttcGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGa                                  >  4:271959/1‑149 (MQ=255)
                                                                                                                 cttcGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGa                                  >  2:261612/1‑149 (MQ=255)
                                                                                                                    cGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATc                                <  6:284405/148‑1 (MQ=255)
                                                                                                                      ccTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGGGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATg                             <  5:329414/149‑1 (MQ=255)
                                                                                                                        tATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCt                           >  3:131002/1‑149 (MQ=255)
                                                                                                                           tgccGAACACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGtt                        >  6:293348/4‑149 (MQ=255)
                                                                                                                             gCGAGCACCCGCCAACCAGCGGACGGCCGAACGCTGGGTGGGGCGTTACTGGGGCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTg                      <  3:295303/149‑1 (MQ=255)
                                                                                                                             gCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGCCGCGCTGGGGCAGACGCTGCTCATGCTGTTTg                      >  8:163669/1‑149 (MQ=255)
                                                                                                                             gCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTg                      >  5:105713/1‑149 (MQ=255)
                                                                                                                               gAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGcc                    <  6:115186/149‑1 (MQ=255)
                                                                                                                                 gCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGt                  >  2:177072/1‑149 (MQ=255)
                                                                                                                                     ccGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCgg               >  2:2371/1‑148 (MQ=255)
                                                                                                                                     ccGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACACTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCgg               >  4:303206/1‑148 (MQ=255)
                                                                                                                                      cGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCa             >  1:3548/1‑149 (MQ=255)
                                                                                                                                       gCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAt            <  3:271959/149‑1 (MQ=255)
                                                                                                                                       gCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAt            <  1:261612/149‑1 (MQ=255)
                                                                                                                                         ctcccAGCGTTCGGCCGTGCGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgt          >  8:334270/4‑149 (MQ=255)
                                                                                                                                          tcccAGCGTTCGGCCGTGCGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgtc         >  2:60899/3‑149 (MQ=255)
                                                                                                                                          tcccAGCGTTCGGCCGTGCGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgtc         >  5:27543/3‑149 (MQ=255)
                                                                                                                                           aCCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgtct        <  3:148735/149‑1 (MQ=255)
                                                                                                                                            ccAGCGTTCGGCCGTGCGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgtctg       >  4:341375/1‑149 (MQ=255)
                                                                                                                                             cAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCAtgtctgt      >  5:118758/1‑149 (MQ=255)
                                                                                                                                                 gcACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCATGTCTGTCAgc  <  2:219462/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCCTGGCCGCCGTCATGCTGTGCTGGTTGGGCTGGGAGCGTTTCGACCCGCAAAGGGTCATGGTCTGGATGACGTTATTGGGCGTGTCGACCGGCGTGCGTACCGCCATGTTCTTCGCCTATCTGCGAGCACCCGCCAACCAGCGCACGGCCGAACGCTGGGAGTGGCGTTACTGGGTCACGCTGATGCTGTCGGCGGCTATCTGGGGCTGGGGCGCCTTTGCCGTAATGCCCGCAGACGACGCGCTGGGGCAGACGCTGATCATGCTGTTTGCCGTCGGCATGTCTGTCAGC  >  NC_002947/3784905‑3785198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑