Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,812,662	T→A	5.2%	I329N (ATT→AAT)	PP_3368 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,812,662	0	T	A	5.2%	243.9 / 2.6	77	I329N (ATT→AAT)	PP_3368	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base A (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

TGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCG  >  NC_002947/3812518‑3812801                                                                                                                                                  |                                                                                                                                            tGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCATGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGcg                                                                                                                                         >  8:182168/1‑148 (MQ=255)    tcTTGGTACTGATGTGGTCGGTCGCCGTTCTGCTGTTCTTTACCTTAGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTTGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGGCAATGTCCAt                                                                                                                                      <  7:364421/148‑1 (MQ=255)    gCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCAt                                                                                                                                      <  5:332349/149‑1 (MQ=255)      ttGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGa                                                                                                                                    <  7:149145/149‑1 (MQ=255)         gtccTGATGTGCTCGGTCGCCGGGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGGCAATGTCCATGAgg                                                                                                                                  <  8:322877/145‑1 (MQ=255)         gTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGgc                                                                                                                                 <  2:331959/149‑1 (MQ=255)             tGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGcc                                                                                                                              >  7:12859/1‑148 (MQ=255)             tGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGcc                                                                                                                              >  7:18629/1‑148 (MQ=255)               aTGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAg                                                                                                                           >  5:240789/1‑149 (MQ=255)                tgtgGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGc                                                                                                                          >  6:33657/1‑149 (MQ=255)                  tgGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCt                                                                                                                        >  5:326359/1‑149 (MQ=255)                   ggtcggtcGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTg                                                                                                                       <  6:326359/149‑1 (MQ=255)                              gTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTc                                                                                                            >  4:90511/1‑149 (MQ=255)                                  tgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTg                                                                                                                       >  1:102379/1‑134 (MQ=255)                                  tgctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTg                                                                                                                       <  2:102379/134‑1 (MQ=255)                                    ctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGGTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACAtt                                                                                                                                            <  5:302549/111‑1 (MQ=255)                                    ctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACAtt                                                                                                                                            >  6:302549/1‑111 (MQ=255)                                    ctgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCa                                                                                                      >  2:15316/1‑149 (MQ=255)                                     tgTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCAt                                                                                                     >  1:37776/1‑149 (MQ=255)                                         cTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcgg                                                                                                 >  1:94956/1‑149 (MQ=255)                                         cTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcgg                                                                                                 >  6:261450/1‑149 (MQ=255)                                           ttACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcggcg                                                                                               >  6:349871/1‑149 (MQ=255)                                            tACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcggc                                                                                                <  5:367711/147‑1 (MQ=255)                                            tACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGt                                                                                              >  5:108607/1‑149 (MQ=255)                                             aCCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGtt                                                                                             >  1:387927/1‑149 (MQ=255)                                              tcTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTTGGCATCTGCCTGGTGGGCATCATGTCCATATCTTCAGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGt                                                                                              <  1:324282/147‑1 (MQ=255)                                               cTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTc                                                                                            >  6:66971/1‑148 (MQ=255)                                               cTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTc                                                                                            >  3:109785/1‑148 (MQ=255)                                               cTTCGCCGCACAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTc                                                                                            >  8:155188/1‑148 (MQ=255)                                                ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                          >  4:253873/1‑149 (MQ=255)                                                ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                          >  3:66561/1‑149 (MQ=255)                                                ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                          >  5:40462/1‑149 (MQ=255)                                                   gccgccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCt                                                                                       <  3:90511/149‑1 (MQ=255)                                                     cgccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTgg                                                                                     <  6:221809/149‑1 (MQ=255)                                                     cgccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTgg                                                                                     <  5:349871/149‑1 (MQ=255)                                                       cccAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGcc                                                                                   <  6:240789/149‑1 (MQ=255)                                                       cccAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                    >  3:364324/1‑148 (MQ=255)                                                         cAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTgg                                                                                     >  4:389082/1‑145 (MQ=255)                                                         cAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTgg                                                                                     <  3:389082/145‑1 (MQ=255)                                                           ggCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgc                                                                                                   <  7:37940/129‑1 (MQ=255)                                                           ggCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgc                                                                                                   >  8:37940/1‑129 (MQ=255)                                                            gcgcTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGc                                                                              >  6:388992/1‑149 (MQ=255)                                                                   ggAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTgggg                                                                       >  7:11313/1‑149 (MQ=255)                                                                    gAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGc                                                                      >  7:98945/1‑149 (MQ=255)                                                                    gAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGc                                                                      >  1:48690/1‑149 (MQ=255)                                                                       ccTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGt                                                                   <  3:189475/149‑1 (MQ=255)                                                                            tgctgGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTg                                                              <  5:261450/149‑1 (MQ=255)                                                                              ctgGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                    >  8:276786/1‑125 (MQ=255)                                                                              ctgGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                    <  7:276786/125‑1 (MQ=255)                                                                              ctgGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                             <  7:155188/148‑1 (MQ=255)                                                                                 ggCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                           <  3:361685/147‑1 (MQ=255)                                                                                 ggCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAgg                                                         <  7:1622/149‑1 (MQ=255)                                                                                 ggCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAg                                                          <  5:33657/148‑1 (MQ=255)                                                                                    aTCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCAt                                                      <  2:48690/149‑1 (MQ=255)                                                                                          cTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCGGTGGATGGGAGGCATGGCGAt                                                >  2:344315/1‑149 (MQ=255)                                                                                              tggGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACt                                            <  8:18629/149‑1 (MQ=255)                                                                                              tggGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACt                                            <  8:12859/149‑1 (MQ=255)                                                                                               gggCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                             >  1:267048/1‑131 (MQ=255)                                                                                               gggCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                             <  2:267048/131‑1 (MQ=255)                                                                                               gggCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTg                                           >  1:199860/1‑149 (MQ=255)                                                                                               gggCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCATCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTg                                           >  6:85902/1‑149 (MQ=255)                                                                                                 gCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGcc                                         >  2:249941/1‑149 (MQ=255)                                                                                                       tGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTa                                                                                                                            <  6:81915/60‑1 (MQ=255)                                                                                                       tGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTa                                                                                                                            >  5:81915/1‑60 (MQ=255)                                                                                                        gATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATgg                                  >  3:255022/1‑149 (MQ=255)                                                                                                          tCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGc                                <  5:388992/149‑1 (MQ=255)                                                                                                             ccT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTgg                             <  1:15316/149‑1 (MQ=255)                                                                                                                  ggcggcACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCATCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGAc                           <  5:85902/147‑1 (MQ=255)                                                                                                                   gcggcACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCa                         <  6:40462/148‑1 (MQ=255)                                                                                                                      gcACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCAc                     <  2:37776/149‑1 (MQ=255)                                                                                                                       cACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCAcc                    <  2:387927/149‑1 (MQ=255)                                                                                                                          cGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGa                 <  1:249941/149‑1 (MQ=255)                                                                                                                                   tCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGGTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgc        <  8:205700/149‑1 (MQ=255)                                                                                                                                    ccGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTgg                             >  5:83483/1‑127 (MQ=255)                                                                                                                                    ccGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGGTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTgg                             <  6:83483/127‑1 (MQ=255)                                                                                                                                     cGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGgcg       <  4:255022/148‑1 (MQ=255)                                                                                                                                      gCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgcc     >  5:53322/1‑149 (MQ=255)                                                                                                                                        cTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc   >  8:193511/1‑149 (MQ=255)                                                                                                                                         tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGCGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCcgcg  >  3:7532/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                            TGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCT‑TGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCG  >  NC_002947/3812518‑3812801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑