Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,853,778 A→G 7.2% intergenic (+61/+21) PP_3401 → / ← PP_3402 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,853,7780AG 7.2% 196.3 / 4.8 70intergenic (+61/+21)PP_3401/PP_3402hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/28);  new base G (2/3);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

TGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGT  >  NC_002947/3853631‑3853926
                                                                                                                                                   |                                                                                                                                                    
tGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGACCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGAc                                                                                                                                                     >  7:185604/1‑149 (MQ=255)
  ggATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGAc                                                                                                                                                     <  4:319957/147‑1 (MQ=255)
  ggATGGGCGGAG‑TATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                                  <  2:323877/149‑1 (MQ=255)
   gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                                  <  1:162310/149‑1 (MQ=255)
   gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCgg                                                                                                                                                  >  1:174365/1‑149 (MQ=255)
    aTGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCGGGGCTGCCCggg                                                                                                                                                 >  3:84410/1‑149 (MQ=255)
                 tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcgc                                                                                                                                    >  2:284483/1‑149 (MQ=255)
                       cGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAg                                                                                                                              <  2:174365/149‑1 (MQ=255)
                        gTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAgg                                                                                                                             <  7:79745/149‑1 (MQ=255)
                        gTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAgg                                                                                                                             >  5:215225/1‑149 (MQ=255)
                         tGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGGCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGc                                                                                                                            <  8:74308/149‑1 (MQ=255)
                             tCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATc                                                                                                                        >  5:40502/1‑149 (MQ=255)
                                cgAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCa                                                                                                                                              >  8:197122/1‑124 (MQ=255)
                                cgAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCa                                                                                                                                              <  7:197122/124‑1 (MQ=255)
                                 gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCt                                                                                                                    <  4:27652/149‑1 (MQ=255)
                                 gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCt                                                                                                                    >  6:386022/1‑149 (MQ=255)
                                  agaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAg                                                                                                                              >  4:103969/1‑138 (MQ=255)
                                  agaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAg                                                                                                                              <  3:103969/138‑1 (MQ=255)
                                     aGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGa                                                                                                                 <  6:35595/148‑1 (MQ=255)
                                      gggAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGt                                                                                                               >  2:119220/1‑149 (MQ=255)
                                       ggAAAGTGCGGCGACGGTGGCCTGAACCTGCTACTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTg                                                                                                              >  1:241924/1‑149 (MQ=255)
                                         aaaGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGACCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGgc                                                                                                            <  8:185604/149‑1 (MQ=255)
                                                ggcgACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGt                                                                                                     >  8:203476/1‑149 (MQ=255)
                                                        tGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCa                                                                                             <  1:49743/149‑1 (MQ=255)
                                                          gCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCac                                                                                            <  8:227927/148‑1 (MQ=255)
                                                           ccTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                                 >  6:317683/1‑142 (MQ=255)
                                                           ccTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                                 <  5:317683/142‑1 (MQ=255)
                                                            cTGAACCTGCTGCTGGAACTGCAATAAGCAGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCAAGACAAGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGCTGGATATCaccc                                                                                          >  4:60668/1‑146 (MQ=255)
                                                               aaCCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgcaac                                                                                       <  5:386022/148‑1 (MQ=255)
                                                                aCCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgcaacgc                                                                                     <  1:119220/149‑1 (MQ=255)
                                                                 ccTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                                  >  2:181852/1‑87 (MQ=39)
                                                                  ctgctgctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCAt                                                                                   >  6:332152/1‑149 (MQ=255)
                                                                        ctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgct                                                                             >  4:143883/1‑149 (MQ=255)
                                                                        ctgGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgct                                                                             <  5:86285/149‑1 (MQ=255)
                                                                          ggAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgctgc                                                                           >  7:367041/1‑149 (MQ=255)
                                                                           gAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgctgct                                                                          >  4:179000/1‑149 (MQ=255)
                                                                             aCTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTc                                                                         >  7:276272/1‑148 (MQ=255)
                                                                                 cAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTc                                                                         >  1:261781/1‑144 (MQ=255)
                                                                                 cAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTc                                                                         <  2:261781/144‑1 (MQ=255)
                                                                                   aTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAgg                                                                  <  6:40502/149‑1 (MQ=255)
                                                                                     aaGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGtt                                                                >  7:181989/1‑149 (MQ=255)
                                                                                       gCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGa                                                              <  8:181989/149‑1 (MQ=255)
                                                                                          gATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGAc                                                             >  4:29743/1‑147 (MQ=255)
                                                                                               gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCa                                                      >  4:119889/1‑149 (MQ=255)
                                                                                                       cGTGGCCTCGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCTCTCAGGCAATCGCGTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGGAGCTGCTCCACCAGGTTGACCAGGTCACGACAACt                                              <  3:60668/149‑1 (MQ=255)
                                                                                                       cGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACt                                              <  6:215225/149‑1 (MQ=255)
                                                                                                        gcagCCACGAGCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTg                                             >  7:146669/4‑149 (MQ=255)
                                                                                                        gTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTg                                             <  1:179987/149‑1 (MQ=255)
                                                                                                           gCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCGCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGtt                                           <  8:184654/148‑1 (MQ=255)
                                                                                                             cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                        <  6:11274/149‑1 (MQ=255)
                                                                                                             cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                        >  5:11274/1‑149 (MQ=255)
                                                                                                                gCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTc                                     >  1:292725/1‑149 (MQ=255)
                                                                                                                gCTAGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACACAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTc                                     >  7:353941/1‑149 (MQ=255)
                                                                                                                    gTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTg                                  <  2:343559/148‑1 (MQ=255)
                                                                                                                      ggCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATc                                                                                              >  4:244944/1‑86 (MQ=255)
                                                                                                                      ggCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATc                                                                                              <  3:244944/86‑1 (MQ=255)
                                                                                                                         tgctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAgcgc                            >  1:348323/1‑149 (MQ=255)
                                                                                                                                 cccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACACAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGt                    <  8:353941/149‑1 (MQ=255)
                                                                                                                                  ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgca                                                                                         <  5:17085/79‑1 (MQ=255)
                                                                                                                                  ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgca                                                                                         >  6:17085/1‑79 (MQ=255)
                                                                                                                                  ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  2:385331/1‑149 (MQ=255)
                                                                                                                                  ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt                   >  5:338829/1‑149 (MQ=255)
                                                                                                                                   cGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                          >  1:296197/1‑109 (MQ=255)
                                                                                                                                   cGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAg                                                          <  2:296197/109‑1 (MQ=255)
                                                                                                                                       cAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAggg              >  6:161381/1‑149 (MQ=255)
                                                                                                                                          ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACt                                              <  3:287185/114‑1 (MQ=255)
                                                                                                                                          ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACt                                              >  4:287185/1‑114 (MQ=255)
                                                                                                                                          ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCa           >  5:981/1‑149 (MQ=255)
                                                                                                                                          ccTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCa           >  3:323374/1‑149 (MQ=255)
                                                                                                                                           cTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAc          >  5:327682/1‑149 (MQ=255)
                                                                                                                                                   aCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTg    <  3:29743/147‑1 (MQ=255)
                                                                                                                                                   aCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTg    <  2:348323/147‑1 (MQ=255)
                                                                                                                                                   aCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGt  <  8:146669/149‑1 (MQ=255)
                                                                                                                                                   aCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGt  >  1:230616/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCACGCCTTGGT  >  NC_002947/3853631‑3853926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑