| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 217,440 | A→G | 6.5% | N7649S (AAC→AGC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 217,440 | 0 | A | G | 6.5% | 175.1 / 2.5 | 61 | N7649S (AAC→AGC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (32/25); new base G (2/2); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
TGAGCAACGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCTGG > NC_002947/217299‑217587 | tGAGCAACGCGTCCAAGTCCGAAGTCACTATCCCCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACaaa > 5:175947/1‑149 (MQ=255)tGAGCAACGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACaaa > 5:193953/1‑149 (MQ=255) aGCAACGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCTCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACACTCAACGACAAAc < 5:398433/148‑1 (MQ=255) aGCAACGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACaaa < 1:94915/147‑1 (MQ=255) aaCGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGTCTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGt < 3:372249/149‑1 (MQ=255) gTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAg > 6:296466/1‑149 (MQ=255) gTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAg > 3:224023/1‑149 (MQ=255) gTCCGAAGTCACTATCACCCTTAGCTCCAGCGGTACCCCCCAAAAAGGTGATGACTTCACCGTTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCTAAGGTACCg < 8:248760/149‑1 (MQ=255) cGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCgag < 4:355349/148‑1 (MQ=255) aaGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAAc > 4:118310/1‑149 (MQ=255) cTATCACCCTTAGCTAGAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTtcgtc < 1:168240/149‑1 (MQ=255) tATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAGCGACAAACTGGTCGAAGGTACCGAGAACTtcgtcg > 7:207009/1‑149 (MQ=255) tCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTcgtcgt > 4:260716/1‑148 (MQ=255) tCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTg < 4:224023/149‑1 (MQ=255) ccTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCAGTGTTGCGACGGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTg > 6:18020/1‑145 (MQ=255) tACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACACACTCGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAAc > 7:337536/1‑149 (MQ=255) tACAGCGGTACAGCCCAAAACGGTGATGACTTCACCGGTGTTTCGACCGTGAAGATTCCGTCCAACAGAACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTACGTAGTGAAGATCGAAAc > 7:116772/1‑149 (MQ=255) cGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGc < 6:104786/147‑1 (MQ=255) cGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGc > 5:104786/1‑147 (MQ=255) tACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTCCCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACgg > 7:109654/1‑149 (MQ=255) tACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACgg > 2:322795/1‑149 (MQ=255) cGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGggtg < 2:30899/149‑1 (MQ=255) aaaaCGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGa < 4:149260/123‑1 (MQ=255) aaaaCGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGa > 3:149260/1‑123 (MQ=255) ggTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCga < 2:328987/149‑1 (MQ=255) cTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTg > 4:84308/1‑148 (MQ=255) aCCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAggg > 8:246231/1‑148 (MQ=255) aCCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGt < 6:193953/149‑1 (MQ=255) aCCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGt < 1:202458/149‑1 (MQ=255) ccGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTc < 3:84308/149‑1 (MQ=255) ggTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGa > 6:109144/1‑149 (MQ=255) ttGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGGCAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATag > 7:1324/1‑148 (MQ=255) ttGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATag > 2:361132/1‑148 (MQ=255) gCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATagcag > 7:211109/1‑149 (MQ=255) gTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGATACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATc > 3:255425/1‑149 (MQ=255) tGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATc > 8:205494/1‑148 (MQ=255) gAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGACGGTACCGAGAACTtc < 1:162072/82‑1 (MQ=255) gAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGACGGTACCGAGAACTtc > 2:162072/1‑82 (MQ=255) aaGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAg > 7:92540/1‑129 (MQ=255) aaGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAg < 8:92540/129‑1 (MQ=255) aaGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAAt > 6:168639/1‑145 (MQ=255) aaGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAAt < 5:168639/145‑1 (MQ=255) aGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGc < 5:18020/149‑1 (MQ=255) aTTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAGCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGt < 8:207009/149‑1 (MQ=255) ttCCGGCCAACAGCACAGGCACGCCGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTg > 5:27476/1‑149 (MQ=255) ttCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGt > 5:323770/1‑148 (MQ=255) cGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTgac > 7:236958/1‑148 (MQ=255) ggCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTgacg < 3:88003/148‑1 (MQ=255) ggCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTgacg > 4:88003/1‑148 (MQ=255) cAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGTAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGacca < 1:322795/149‑1 (MQ=255) cAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAg < 4:33849/115‑1 (MQ=255) cAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAg > 3:33849/1‑115 (MQ=255) gCACAGGCACGACGTTCAACATCGCTACCCTCAGCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGggtg > 6:91137/1‑93 (MQ=255) gCACAGGCACGACGTTCAACATCGCTACCCTCAGCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGggtg < 5:91137/93‑1 (MQ=255) ggCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGa < 3:260716/149‑1 (MQ=255) gacgTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATg < 8:236958/149‑1 (MQ=255) cgTTCAAAATTGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAAGTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCAGTCTCGACAATGac > 6:165829/1‑149 (MQ=255) aaCATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCt < 7:205494/149‑1 (MQ=255) ccTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGc < 3:118310/149‑1 (MQ=255) tCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCt > 3:144725/1‑149 (MQ=255) tCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCt > 6:122291/1‑149 (MQ=255) caCGACAAACTGGTCGAAGTTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAAACTGCAGGTCGATAGCAGCAAATAGAGCGTGACTACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCt < 8:116772/146‑1 (MQ=255) aaCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCTgg > 3:375838/1‑149 (MQ=255) aCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAAt > 2:44134/1‑125 (MQ=255) aCGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAAt < 1:44134/125‑1 (MQ=255) | TGAGCAACGCGTCCAAGTCCGAAGTCACTATCACCCTTAGCTACAGCGGTACCGCCCAAAACGGTGATGACTTCACCGGTGTTGCGACCGTGAAGATTCCGGCCAACAGCACAGGCACGACGTTCAACATCGCTACCCTCAACGACAAACTGGTCGAAGGTACCGAGAACTTCGTCGTGAAGATCGAAACCGCCACGGGTGGTAACTTCGAGAACCTGCAGGTCGATAGCAGCAAATCGAGCGTGACGACCACCATTCTCGACAATGACCACCTGCCGGTTTCGCCTGG > NC_002947/217299‑217587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |