Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,144,163 C→A 8.2% A65D (GCC→GAC)  PP_3648 → carboxymuconolactone decarboxylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,144,1630CA 8.2% 142.4 / 4.3 49A65D (GCC→GAC) PP_3648carboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

GTACTGGGCGAGGACTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCGCGGCGGTGGACAGT  >  NC_002947/4144015‑4144305
                                                                                                                                                    |                                                                                                                                              
gTACTGGGCGAGGACTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGc                                                                                                                                                <  4:107838/149‑1 (MQ=255)
      ggCGAGGACTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGa                                                                                                                                           <  7:50793/148‑1 (MQ=255)
            gACTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCcgc                                                                                                                                    <  5:235857/149‑1 (MQ=255)
             aCTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGACATGATTTCcgcg                                                                                                                                   >  3:134192/1‑149 (MQ=255)
             aCTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGACATGATTTCcgcg                                                                                                                                   >  3:128378/1‑149 (MQ=255)
                  gTCAACCGCTCGATCCAGAACGCAGATGGATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCaa                                                                                                                              <  1:141971/149‑1 (MQ=255)
                           tCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCa                                                                                                                     >  5:65995/1‑149 (MQ=255)
                             gATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCAc                                                                                                                    <  3:97543/148‑1 (MQ=255)
                                ccAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGc                                                                                                                >  5:211212/1‑149 (MQ=255)
                                 cAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGc                                                                                                                <  8:325734/148‑1 (MQ=255)
                                       gCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGtt                                                                                                         <  7:73386/149‑1 (MQ=255)
                                           aTGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTAACGTCTGGGGCCGCGAAGGCTTGTAGCTGAAAGAGCGCAGCATGATAAAGTTGGCCATGAGTTCCTCGCTCAACAGGCCCCACGAGAACAAGttc                                                                                                        >  8:214051/1‑145 (MQ=255)
                                                              gCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTgcgc                                                                                   >  4:17620/1‑148 (MQ=255)
                                                                aGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTc                                                                                                                                <  7:194709/101‑1 (MQ=255)
                                                                aGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTc                                                                                                                                >  8:194709/1‑101 (MQ=255)
                                                                 ggAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATg                                                                               <  7:219298/149‑1 (MQ=255)
                                                                  gAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATgg                                                                              <  3:49657/149‑1 (MQ=255)
                                                                  gAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATgg                                                                              >  3:310717/1‑149 (MQ=255)
                                                                   aGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATggg                                                                             <  3:17620/149‑1 (MQ=255)
                                                                        gTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGa                                                                         <  6:193814/148‑1 (MQ=255)
                                                                        gTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGa                                                                         <  2:118060/148‑1 (MQ=255)
                                                                          cACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGcc                                                                      >  3:368879/1‑149 (MQ=255)
                                                                           aCCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCg                                                                     >  3:139494/1‑149 (MQ=255)
                                                                           aCCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCg                                                                     >  3:145312/1‑149 (MQ=255)
                                                                                gTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAAc                                                                <  2:147843/149‑1 (MQ=255)
                                                                                         gggTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGACATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTcgc                                                        <  4:128378/148‑1 (MQ=255)
                                                                                         gggTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGACATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTcgc                                                        <  4:134192/148‑1 (MQ=255)
                                                                                             cACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTc                                                                                                 <  1:136329/103‑1 (MQ=255)
                                                                                             cACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTc                                                                                                 >  2:136329/1‑103 (MQ=255)
                                                                                               cGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATcc                                                 >  2:328052/1‑149 (MQ=255)
                                                                                                 tCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTg                                               >  4:127618/1‑149 (MQ=255)
                                                                                                     gggCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTc                                            <  6:211212/148‑1 (MQ=255)
                                                                                                        ccGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAgg                                        >  6:297205/1‑149 (MQ=255)
                                                                                                                  ttGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTa                              >  4:192318/1‑149 (MQ=255)
                                                                                                                     tCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTAtt                            <  4:310717/148‑1 (MQ=255)
                                                                                                                       gCTGAAAGAGCGCAGCATGATAAACTTCGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGAGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTgcg                         >  7:286721/1‑149 (MQ=255)
                                                                                                                        cTGAAAGAGCGCATCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGTTGCCCTGCGCAATGGGCTGAGCCGTGAACATATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTgcgg                        <  7:214051/149‑1 (MQ=255)
                                                                                                                        cTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTgcgg                        <  6:65995/149‑1 (MQ=255)
                                                                                                                           aaaGAGCGCAGCATGATAAACTTGGGCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTgcggcg                      >  8:231039/1‑148 (MQ=255)
                                                                                                                           aaaGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTgcggc                       <  3:127618/147‑1 (MQ=255)
                                                                                                                                   cagcaTGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGc                                              >  7:273937/1‑116 (MQ=255)
                                                                                                                                   cagcaTGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGc                                              <  8:273937/116‑1 (MQ=255)
                                                                                                                                   cagcaTGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCgcgg             >  8:71163/1‑149 (MQ=255)
                                                                                                                                       aTGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCGCGGCGGt         <  5:297205/149‑1 (MQ=255)
                                                                                                                                          aTAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCGCGGCGGTgg       <  1:328052/148‑1 (MQ=255)
                                                                                                                                           tAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCgcggc            >  6:92474/1‑142 (MQ=255)
                                                                                                                                           tAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCgcggc            <  5:92474/142‑1 (MQ=255)
                                                                                                                                              aCTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCGCGGCGGTGGACAGt  >  3:12823/1‑149 (MQ=255)
                                                                                                                                                ttGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTcgcg                                                                                              >  6:285193/1‑55 (MQ=255)
                                                                                                                                                ttGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTcgcg                                                                                              <  5:285193/55‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GTACTGGGCGAGGACTACGTCAACCGCTCGATCCAGAACGCAGATGAATTCACCAAACCGTTGCAGGAGCTGGTCACCGAGTATTGCTGGGGTCACGTCTGGGGCCGCGAAGGCTTGTCGCTGAAAGAGCGCAGCATGATAAACTTGGCCATGATTTCCGCGCTCAACAGGCCCCACGAGCTCAAGTTGCACATTCGCGGTGCCCTGCGCAATGGGCTGAGCCGTGAACAGATTCGCGAAATCCTGCTCCAGGTCGGCATTTATTGCGGCGTACCCGCGGCGGTGGACAGT  >  NC_002947/4144015‑4144305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑