Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,205,671 G→A 7.1% A702V (GCA→GTA)  PP_3691 ← DNA helicase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,205,6710GA 7.1% 166.3 / 2.2 56A702V (GCA→GTA) PP_3691DNA helicase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (22/30);  new base A (2/2);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATACACCACATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGG  >  NC_002947/4205524‑4205811
                                                                                                                                                   |                                                                                                                                            
cgATACACCACATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTTCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGACTTAGGCTGc                                                                                                                                             >  4:246767/1‑149 (MQ=255)
    acacCACATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGcc                                                                                                                                         <  7:218797/149‑1 (MQ=255)
     caccacATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGcc                                                                                                                                         <  5:264019/148‑1 (MQ=255)
      accacATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCAt                                                                                                                                       <  4:180352/149‑1 (MQ=255)
          caTCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTACGGCCATCAcc                                                                                                                                   >  8:238902/1‑149 (MQ=255)
          caTCCAGCGCGGCGATATTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTCGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTACGGCCATAAcc                                                                                                                                   >  8:232832/1‑149 (MQ=255)
            tCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCAccgc                                                                                                                                 >  2:307859/1‑149 (MQ=255)
                  gcgtcgATCTTTTCCTAGACGAACAGCACACGACGCCCGTTGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGaaa                                                                                                                            <  8:244439/148‑1 (MQ=255)
                  gcggcgATC‑TTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGt                                                                                                                          <  1:213411/149‑1 (MQ=255)
                    ggcgATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGtt                                                                                                                         <  4:158552/149‑1 (MQ=255)
                      cgATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTga                                                                                                                       <  4:99971/149‑1 (MQ=255)
                      cgATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTga                                                                                                                       <  5:370837/149‑1 (MQ=255)
                      cgATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACGGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTga                                                                                                                       <  5:92914/149‑1 (MQ=255)
                             ttCCGCGACCAACCGCACACGACGCCCCTGCGCAAGCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAgagga                                                                                                                  <  8:114856/147‑1 (MQ=255)
                             ttCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTgaga                                                                                                                     <  2:9120/144‑1 (MQ=255)
                               ccGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTc                                                                                                               <  5:227724/148‑1 (MQ=255)
                                      gAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATTGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTgaga                                                                                                                     <  2:394712/135‑1 (MQ=255)
                                        aCAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGt                                                                                                     <  3:246767/149‑1 (MQ=255)
                                        aCAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGt                                                                                                     >  2:343810/1‑149 (MQ=255)
                                        aCAGCACACGACGCCCCTGGGCAATCGACTGAGAGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGtt                                                                                                                         <  7:212828/129‑1 (MQ=255)
                                        aCAGCACACGACGCCCCTGGGCAATCGACTGAGAGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGtt                                                                                                                         >  8:212828/1‑129 (MQ=255)
                                           gCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTggg                                                                                                  <  2:199918/149‑1 (MQ=255)
                                               acgacgCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCag                                                                                               >  7:236851/1‑148 (MQ=255)
                                                cgacgCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCagaa                                                                                             <  3:330036/149‑1 (MQ=255)
                                                    gCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACg                                                                                         >  5:319400/1‑149 (MQ=255)
                                                             cAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGc                                                                                <  1:343810/149‑1 (MQ=255)
                                                                        gCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACg                                                                     <  5:57495/149‑1 (MQ=255)
                                                                              aTGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAg                                                               >  5:19131/1‑149 (MQ=255)
                                                                                 ttGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGACGGCCCATAGTCACGATCCAGCg                                                             >  4:243761/1‑148 (MQ=255)
                                                                                          gTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCt                                                    <  6:19131/148‑1 (MQ=255)
                                                                                            cTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAg                                                 >  3:184445/1‑149 (MQ=255)
                                                                                             tGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATACAGCGACTCGGCCTCAgg                                                >  6:272106/1‑149 (MQ=255)
                                                                                               gCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAg                                                 <  1:307859/146‑1 (MQ=255)
                                                                                                     gCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATc                                                                  >  4:89937/1‑123 (MQ=255)
                                                                                                     gCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATc                                                                  <  3:89937/123‑1 (MQ=255)
                                                                                                     gCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGa                                         <  8:236851/148‑1 (MQ=255)
                                                                                                      ccGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTg                                                                                                                        <  3:194169/68‑1 (MQ=255)
                                                                                                      ccGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTg                                                                                                                        >  4:194169/1‑68 (MQ=255)
                                                                                                         gTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATc                                    <  5:272106/149‑1 (MQ=255)
                                                                                                                   ggCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAaacgaac                          <  6:319400/149‑1 (MQ=255)
                                                                                                                        aTGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAAt                      >  8:342934/1‑148 (MQ=255)
                                                                                                                         tGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCa                    >  7:273011/1‑149 (MQ=255)
                                                                                                                           aGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCAc                   <  4:184445/148‑1 (MQ=255)
                                                                                                                           aGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACg                  <  5:72083/149‑1 (MQ=255)
                                                                                                                            ttACAAAGTCTTTGCCTTTTGCTACGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgt                 <  7:232832/148‑1 (MQ=255)
                                                                                                                            gTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgt                 >  3:36449/1‑149 (MQ=255)
                                                                                                                            gTACAAAGTCTTTGCCTTTGGCTACGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgt                 <  7:238902/149‑1 (MQ=255)
                                                                                                                             tACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCag                                                                                               >  3:378430/1‑70 (MQ=255)
                                                                                                                                aaaGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgtggtg             >  3:38013/1‑149 (MQ=255)
                                                                                                                                aaaGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgtggt              >  4:367361/1‑148 (MQ=255)
                                                                                                                                aaaGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACgtggt              <  3:367361/148‑1 (MQ=255)
                                                                                                                                  aGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAATGGAGTATCCGAAACGAACGAATCACGTGGTGTa           >  3:48597/1‑149 (MQ=255)
                                                                                                                                      tttGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATc         >  1:216915/1‑147 (MQ=255)
                                                                                                                                          ccTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAAt                      >  7:164758/1‑130 (MQ=255)
                                                                                                                                          ccTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAAt                      <  8:164758/130‑1 (MQ=255)
                                                                                                                                           cTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCAAGTGGTGTATCCAGCAgg  >  5:216176/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CGATACACCACATCCAGCGCGGCGATCTTTTCCGAGACGAACAGCACACGACGCCCCTGGGCAATCGACTGAGCGATCATGTTGGAGATGGTCTGGCTCTTGCCGGTGCCAGGTGGGCCGATGAGTACAAAGTCTTTGCCTTTGGCTGCGGCCATCACCGCCGAAAGTTGAGAGGAGTCAGATGGCAGTGGGCAGAAGACGTCGGTCGGCCCATAGTCACGATCCAGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGG  >  NC_002947/4205524‑4205811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑