Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,273,050 G→T 8.0% intergenic (+26/+35) PP_3743 → / ← glcC hypothetical protein/transcriptional regulator GlcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,273,0500GT 8.0% 142.4 / 4.0 50intergenic (+26/+35)PP_3743/glcChypothetical protein/transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base T (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

GCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGC  >  NC_002947/4272906‑4273198
                                                                                                                                                |                                                                                                                                                    
gcgcCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGt                                                                                                                                                  >  1:143183/1‑149 (MQ=255)
         tgGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                         <  7:158951/149‑1 (MQ=255)
         gggAGTCGGCGCGTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                         <  4:163954/148‑1 (MQ=255)
          ggAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCgcggcg                                                                                                                                                    <  8:294981/137‑1 (MQ=255)
          ggAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCgcggcg                                                                                                                                                    >  7:294981/1‑137 (MQ=255)
          ggAGTCGGCGCTTGAACAGGCCCGCAACGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCCGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGACTAAACCTGTACCGCCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                           >  4:305387/1‑146 (MQ=255)
           gAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                         >  5:347197/1‑147 (MQ=255)
           gAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                         >  3:137463/1‑147 (MQ=255)
             gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                     <  4:176692/149‑1 (MQ=38)
                ggCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGcc                                                                                                                                  >  2:210687/1‑149 (MQ=255)
                  cgcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGa                                                                                                                                <  6:347197/149‑1 (MQ=255)
                   gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                     <  3:12561/143‑1 (MQ=255)
                   gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                     >  4:12561/1‑143 (MQ=255)
                   gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGa                                                                                                                                >  8:334924/1‑148 (MQ=255)
                        aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                           <  3:339055/148‑1 (MQ=255)
                        aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                           <  7:334924/148‑1 (MQ=255)
                        aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                           <  4:285554/148‑1 (MQ=255)
                               ccGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCtt                                                                                                                   >  5:340696/1‑149 (MQ=255)
                                cGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTc                                                                                                                  <  6:308993/149‑1 (MQ=255)
                                 gCAAGGACCATCGTGCGCGGCTGGCCTACGACGATGAGCTGGCCGGGGCCATCCTGTCGCAAGATGTGCGTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAGGGGTGAGGGCCGATACAAGGCCGCTTCa                                                                                                                 <  8:330886/149‑1 (MQ=255)
                                       aCCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCa                                                                                                           <  2:152609/149‑1 (MQ=255)
                                             cTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGccatc                                                                                                     <  3:19849/149‑1 (MQ=255)
                                                      aGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCAt                                                                                            <  1:210687/149‑1 (MQ=255)
                                                        gCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACGTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGTCATCCATACGCATgg                                                                                          <  4:137463/149‑1 (MQ=255)
                                                               acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCt                                                                                   <  3:208499/149‑1 (MQ=255)
                                                                 gaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACGATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGa                                                                                 >  8:192152/1‑149 (MQ=255)
                                                                       cgggCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTTACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAgg                                                                            <  7:243965/146‑1 (MQ=255)
                                                                       cTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGgc                                                                           >  3:229046/1‑149 (MQ=255)
                                                                          gCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCATTTAGCGGCGGTTCGACGCGAGAGGGCAGATACAAGGCGCGTACACACCCAGCCAACAAAACGCATGGGAGCCCGGACCAGgca                                                                          >  8:243965/1‑146 (MQ=255)
                                                                           cccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCgctg                                                                       <  8:258756/149‑1 (MQ=255)
                                                                           cccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACAAGGCgctg                                                                       >  3:57663/1‑149 (MQ=255)
                                                                              gggCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGa                                                                                 >  4:159824/1‑136 (MQ=255)
                                                                                 ccATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTc                                                                 <  2:143183/149‑1 (MQ=255)
                                                                                         tCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGa                                                         >  7:154975/1‑149 (MQ=255)
                                                                                          cGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAt                                                        >  2:163210/1‑149 (MQ=255)
                                                                                               gATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTccc                                                   >  3:359851/1‑149 (MQ=255)
                                                                                                   aCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCa                                                >  7:303554/1‑148 (MQ=255)
                                                                                                   aCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCa                                                >  7:99317/1‑148 (MQ=255)
                                                                                                         tgGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTc                                         <  6:338598/149‑1 (MQ=255)
                                                                                                             tGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGt                                     >  1:4024/1‑149 (MQ=255)
                                                                                                               aCCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTa                                    <  5:93024/148‑1 (MQ=255)
                                                                                                                ccATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATg                                  <  8:154975/149‑1 (MQ=255)
                                                                                                                 cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGCCCAGGCGCTGCTCTTCCTGCTCGAGCTCCCGCAAACTGTCGCGTATGc                                 >  6:91413/1‑149 (MQ=255)
                                                                                                                 cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGc                                 >  1:347881/1‑149 (MQ=255)
                                                                                                                  aTTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGcc                                >  5:125128/1‑149 (MQ=255)
                                                                                                                      aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                             <  7:192152/148‑1 (MQ=255)
                                                                                                                      aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                             >  3:158048/1‑148 (MQ=255)
                                                                                                                        tAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGat                          <  3:358511/149‑1 (MQ=255)
                                                                                                                          aaCCTGTACCGTCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAATGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGata                         <  7:363059/148‑1 (MQ=255)
                                                                                                                          aaCCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGatat                        <  1:163210/149‑1 (MQ=255)
                                                                                                                            ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGc                      <  3:305387/149‑1 (MQ=255)
                                                                                                                                 atcGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAcg                 >  7:21238/3‑149 (MQ=38)
                                                                                                                                                gcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTTCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTgcgc  <  4:359851/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGC  >  NC_002947/4272906‑4273198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑