Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 268,744 T→A 9.1% intergenic (‑75/+25) PP_0216 ← / ← desA sensory box/GGDEF family protein/delta‑9 fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947268,7440TA 9.1% 189.6 / 7.9 74intergenic (‑75/+25)PP_0216/desAsensory box/GGDEF family protein/delta‑9 fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base T (31/35);  new base A (5/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

GGGGAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTGGCCG  >  NC_002947/268598‑268892
                                                                                                                                                  |                                                                                                                                                    
ggggAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTgg                                                                                                                                                    >  2:98100/1‑149 (MQ=255)
   gAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTggggg                                                                                                                                                 <  3:155106/149‑1 (MQ=255)
     aaaTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGc                                                                                                                                               <  7:193523/149‑1 (MQ=255)
      aaTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTggg                                                                                                                                                   <  2:313404/144‑1 (MQ=255)
      aaTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTggg                                                                                                                                                   >  1:313404/1‑144 (MQ=255)
      aaTCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCg                                                                                                                                              >  6:176329/1‑149 (MQ=255)
              gAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGAt                                                                                                                                       >  4:90526/1‑148 (MQ=255)
              gAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGAt                                                                                                                                       <  2:238052/148‑1 (MQ=255)
               aaTGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATc                                                                                                                                      >  7:34596/1‑148 (MQ=255)
               aaTGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCa                                                                                                                                     <  5:176329/149‑1 (MQ=255)
               aaTGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAACGGCACGCCCCCCAGCGGGGCGTGccattcg                                                                                                                                     <  3:316621/149‑6 (MQ=255)
                  gTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATc                                                                                                                                      <  1:318366/145‑1 (MQ=255)
                  gTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATc                                                                                                                                      >  2:318366/1‑145 (MQ=255)
                      ttGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggcg                                                                                                                               >  1:90265/1‑148 (MQ=255)
                        ggCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggcg                                                                                                                               <  2:90265/146‑1 (MQ=255)
                        ggCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGt                                                                                                                             >  7:220608/1‑148 (MQ=255)
                         gCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCa                                                                                                                           <  2:6713/149‑1 (MQ=255)
                          ccctgtgCACGGAGCATGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAg                                                                                                                          <  8:116225/146‑1 (MQ=255)
                          ccATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAg                                                                                                                          <  2:123205/149‑1 (MQ=255)
                          ccATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAg                                                                                                                          <  2:251908/149‑1 (MQ=255)
                              gtgCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggc                                                                                                                                >  6:351512/1‑139 (MQ=255)
                              gtgCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAcggc                                                                                                                                <  5:351512/139‑1 (MQ=255)
                                gCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCg                                                                                                                    >  1:266182/1‑149 (MQ=255)
                                    ggAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCg                                                                                                                <  6:250745/149‑1 (MQ=255)
                                     gAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCgg                                                                                                               <  7:253814/149‑1 (MQ=255)
                                           gTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAg                                                                                                         >  2:187342/1‑149 (MQ=255)
                                                aaCGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGTAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAg                                                                                                     >  1:301379/1‑148 (MQ=255)
                                                aaCGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAg                                                                                                     >  1:230088/1‑148 (MQ=255)
                                                 aCGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTa                                                                                                   <  3:90526/149‑1 (MQ=255)
                                                  cGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGTAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTaa                                                                                                  <  2:301379/149‑1 (MQ=255)
                                                  cGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTaa                                                                                                  <  8:29653/149‑1 (MQ=255)
                                                  cGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTaa                                                                                                  <  2:266182/149‑1 (MQ=255)
                                                    tGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGt                                                                                                >  3:22197/1‑149 (MQ=255)
                                                     gAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTc                                                                                               >  8:42372/1‑149 (MQ=255)
                                                      aaaGCGTGCGGCAAGGATGCGGCCCGCCACGGATGCGATATTCGGGATTCGAGTGGAGAGTTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAAGGGCGGTCAGGCGCCGGTCGGGCGCAGCGTGTATGTCt                                                                                              <  4:179406/149‑1 (MQ=255)
                                                       aaGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCtt                                                                                             >  6:256842/1‑149 (MQ=255)
                                                       aaGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCtt                                                                                             <  1:98100/149‑1 (MQ=255)
                                                       aaGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCtt                                                                                             <  5:129300/149‑1 (MQ=255)
                                                        aGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCTGCGAGTAAGTCTTg                                                                                            <  8:34596/149‑1 (MQ=255)
                                                        aGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTaa                                                                                                  >  2:88783/1‑143 (MQ=255)
                                                        aGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTaa                                                                                                  <  1:88783/143‑1 (MQ=255)
                                                              cGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTtg                                                                                       <  6:145056/148‑1 (MQ=255)
                                                                gCCAGCATCCGGCCCGCCACGCAGGAGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgc                                                                                    >  4:56822/1‑149 (MQ=255)
                                                                cccAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCTAATGGCACGCCCCGCTGGGGGGCGAGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCTGCGAGTAACTCTTGAGTtgtg                                                                                     >  8:279764/2‑148 (MQ=255)
                                                                   aGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGtgctgc                                                                                 >  4:238150/1‑149 (MQ=255)
                                                                     cATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCg                                                                                >  3:251857/1‑148 (MQ=255)
                                                                      aTCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGGGGAGAGGTGGGGGCGTAGTTCCGACTGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGaa                                                                              <  4:7807/149‑1 (MQ=255)
                                                                      aTCCGGCCCGCCACGCAGGAGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGaa                                                                              <  3:56822/149‑1 (MQ=255)
                                                                          ggCCCGCCCCGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCAGAATGGCACGCCCCGCAGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGAGCAGCGAGTAAGTCGTGAGATGTGCTGCGAAGTcg                                                                          >  8:328019/1‑149 (MQ=255)
                                                                              cGCCACGCAGGCGATTTTCCTTATTAGTGTGGTGAGGTGGGGGCGAAGTTCCGATTGGCACGCCCCTCTGTGGGGCGTGCGGATCATCGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTTTGCTGCGAAGTCGCGCa                                                                      <  8:229705/149‑1 (MQ=255)
                                                                                ccACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGt                                                                    <  8:220608/149‑1 (MQ=255)
                                                                                     cAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTg                                                               <  1:187342/149‑1 (MQ=255)
                                                                                      aGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTtgt                                                                                      <  8:316388/125‑1 (MQ=255)
                                                                                      aGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTtgt                                                                                      >  7:316388/1‑125 (MQ=255)
                                                                                       ggCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGa                                                             >  2:6534/1‑149 (MQ=255)
                                                                                            tatTCCGGACTCGAGCGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCg                                                        <  1:365311/149‑1 (MQ=255)
                                                                                                    aTTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTc                                                >  6:39499/1‑149 (MQ=255)
                                                                                                          gTGGAGAGGTGGTGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGAGCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGcc                                            <  7:142170/147‑1 (MQ=255)
                                                                                                            ggAGATGTTGTGGCGAAGTTCCGAATGGCAAGCCCCGCTGGGGGGCGTGCCGATCAACGGCGTTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGATTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCa                                         <  7:328019/148‑1 (MQ=255)
                                                                                                             gagaGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCAt                                        <  7:42372/148‑1 (MQ=255)
                                                                                                              agagGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATg                                       >  1:151583/1‑148 (MQ=255)
                                                                                                               gagGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTa                                     >  4:13483/1‑149 (MQ=255)
                                                                                                                  gTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTAcc                                   >  5:172773/1‑148 (MQ=255)
                                                                                                                   tGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTAcc                                   >  7:100729/1‑147 (MQ=255)
                                                                                                                                     cggCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAg               >  4:220716/2‑149 (MQ=255)
                                                                                                                                       gCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATgg                      <  7:41970/140‑1 (MQ=255)
                                                                                                                                       gCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATgg                      >  8:41970/1‑140 (MQ=255)
                                                                                                                                       gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCAt                        >  5:302106/1‑138 (MQ=255)
                                                                                                                                       gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCAt                        <  6:302106/138‑1 (MQ=255)
                                                                                                                                       gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCa             >  1:6236/1‑149 (MQ=255)
                                                                                                                                       gCACGCCCCCCAGCGGGGCGTGCCGATCAACGGCGGTCAGGCGACGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCa             >  8:235033/1‑149 (MQ=255)
                                                                                                                                         aCGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATg           >  7:329429/1‑149 (MQ=255)
                                                                                                                                              ccGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTg      >  7:221916/1‑149 (MQ=255)
                                                                                                                                              ccGCGGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCATGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTg      <  2:230088/149‑1 (MQ=255)
                                                                                                                                                  tGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTCCCCAGTCTTTCATGGCTGCCAGCATGTCGTGGCCg  >  8:98106/1‑149 (MQ=255)
                                                                                                                                                  tGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGc                  >  5:135989/1‑133 (MQ=255)
                                                                                                                                                  tGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGc                  <  6:135989/133‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGGGAAAATCGTTGGAATGTTCTTGGCCATGTGCACGGAGCAGGTCTCAACGTGAAAGCGTGCGGCCAGCATCCGGCCCGCCACGCAGGCGATATTCCGGATTCGAGTGGAGAGGTGGGGGCGAAGTTCCGAATGGCACGCCCCGCTGGGGGGCGTGCCGATCAACGGCGGTCAGGCGCCGGTCGGGCGCAGCGAGTAAGTCTTGAGTTGTGCTGCGAAGTCGCGCAGTGCCTGGATGCCGCTGGTTTCGGCCTCATGTACCCAGTCTTTCATGGCTGCCAGCATGTCGTGGCCG  >  NC_002947/268598‑268892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑