Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,273,058 A→C 7.0% intergenic (+34/+27) PP_3743 → / ← glcC hypothetical protein/transcriptional regulator GlcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,273,0580AC 7.0% 143.2 / 2.5 53intergenic (+34/+27)PP_3743/glcChypothetical protein/transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base A (21/28);  new base C (2/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGG  >  NC_002947/4272915‑4273206
                                                                                                                                               |                                                                                                                                                    
tgGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                                 <  7:158951/149‑1 (MQ=255)
gggAGTCGGCGCGTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                                 <  4:163954/148‑1 (MQ=255)
 ggAGTCGGCGCTTGAACAGGCCCGCAACGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCCGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGACTAAACCTGTACCGCCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                                   >  4:305387/1‑146 (MQ=255)
  gAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                                 >  5:347197/1‑147 (MQ=255)
  gAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCga                                                                                                                                                 >  3:137463/1‑147 (MQ=255)
    gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                             <  4:176692/149‑1 (MQ=38)
       ggCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGcc                                                                                                                                          >  2:210687/1‑149 (MQ=255)
         cgcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGa                                                                                                                                        <  6:347197/149‑1 (MQ=255)
          gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                             >  4:12561/1‑143 (MQ=255)
          gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                             <  3:12561/143‑1 (MQ=255)
          gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGa                                                                                                                                        >  8:334924/1‑148 (MQ=255)
               aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                                   <  7:334924/148‑1 (MQ=255)
               aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                                   <  4:285554/148‑1 (MQ=255)
               aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                                   <  3:339055/148‑1 (MQ=255)
                      ccGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCtt                                                                                                                           >  5:340696/1‑149 (MQ=255)
                       cGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTc                                                                                                                          <  6:308993/149‑1 (MQ=255)
                        gCAAGGACCATCGTGCGCGGCTGGCCTACGACGATGAGCTGGCCGGGGCCATCCTGTCGCAAGATGTGCGTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAGGGGTGAGGGCCGATACAAGGCCGCTTCa                                                                                                                         <  8:330886/149‑1 (MQ=255)
                              aCCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCa                                                                                                                   <  2:152609/149‑1 (MQ=255)
                                    cTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGccatc                                                                                                             <  3:19849/149‑1 (MQ=255)
                                             aGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCAt                                                                                                    <  1:210687/149‑1 (MQ=255)
                                               gCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACGTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGTCATCCATACGCATgg                                                                                                  <  4:137463/149‑1 (MQ=255)
                                                      acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCt                                                                                           <  3:208499/149‑1 (MQ=255)
                                                        gaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACGATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGa                                                                                         >  8:192152/1‑149 (MQ=255)
                                                              cgggCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTTACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAgg                                                                                    <  7:243965/146‑1 (MQ=255)
                                                              cTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGgc                                                                                   >  3:229046/1‑149 (MQ=255)
                                                                 gCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCATTTAGCGGCGGTTCGACGCGAGAGGGCAGATACAAGGCGCGTACACACCCAGCCAACAAAACGCATGGGAGCCCGGACCAGgca                                                                                  >  8:243965/1‑146 (MQ=255)
                                                                  cccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCgctg                                                                               <  8:258756/149‑1 (MQ=255)
                                                                  cccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACAAGGCgctg                                                                               >  3:57663/1‑149 (MQ=255)
                                                                     gggCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGa                                                                                         >  4:159824/1‑136 (MQ=255)
                                                                        ccATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTc                                                                         <  2:143183/149‑1 (MQ=255)
                                                                                tCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGa                                                                 >  7:154975/1‑149 (MQ=255)
                                                                                 cGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAt                                                                >  2:163210/1‑149 (MQ=255)
                                                                                      gATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTccc                                                           >  3:359851/1‑149 (MQ=255)
                                                                                          aCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCa                                                        >  7:303554/1‑148 (MQ=255)
                                                                                          aCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCa                                                        >  7:99317/1‑148 (MQ=255)
                                                                                                tgGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTc                                                 <  6:338598/149‑1 (MQ=255)
                                                                                                    tGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGt                                             >  1:4024/1‑149 (MQ=255)
                                                                                                      aCCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTa                                            <  5:93024/148‑1 (MQ=255)
                                                                                                       ccATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATg                                          <  8:154975/149‑1 (MQ=255)
                                                                                                        cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGCCCAGGCGCTGCTCTTCCTGCTCGAGCTCCCGCAAACTGTCGCGTATGc                                         >  6:91413/1‑149 (MQ=255)
                                                                                                        cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGc                                         >  1:347881/1‑149 (MQ=255)
                                                                                                         aTTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGcc                                        >  5:125128/1‑149 (MQ=255)
                                                                                                             aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                                     >  3:158048/1‑148 (MQ=255)
                                                                                                             aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                                     <  7:192152/148‑1 (MQ=255)
                                                                                                               tAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGat                                  <  3:358511/149‑1 (MQ=255)
                                                                                                                 aaCCTGTACCGTCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAATGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGata                                 <  7:363059/148‑1 (MQ=255)
                                                                                                                 aaCCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGatat                                <  1:163210/149‑1 (MQ=255)
                                                                                                                   ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGc                              <  3:305387/149‑1 (MQ=255)
                                                                                                                        atcGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAcg                         >  7:21238/3‑149 (MQ=38)
                                                                                                                                       gcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTTCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTgcgc          <  4:359851/149‑1 (MQ=255)
                                                                                                                                         ggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCt        <  2:4024/149‑1 (MQ=255)
                                                                                                                                              gACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCg   <  8:303554/149‑1 (MQ=255)
                                                                                                                                              gACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTCGATATGCTCACGCGCCGCCCGCTGCGCCTGCTc    <  6:340696/148‑1 (MQ=255)
                                                                                                                                               aCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCgg  >  8:327548/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGGTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCTGCTCGG  >  NC_002947/4272915‑4273206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑