Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,316,022 A→C 14.6% A35A (GCA→GCC)  PP_3788 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,316,0220AC14.6% 130.3 / 15.1 54A35A (GCA→GCC) PP_3788non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (29/17);  new base C (3/5);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

TCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGT  >  NC_002947/4315876‑4316168
                                                                                                                                                  |                                                                                                                                                  
tcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGc                                                                                                                                                  >  5:125627/1‑149 (MQ=255)
tcaGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGc                                                                                                                                                  <  3:77413/149‑1 (MQ=255)
  aGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                                 <  6:280843/148‑1 (MQ=255)
          aGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgat                                                                                                                                        <  6:202834/149‑2 (MQ=255)
          aGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgat                                                                                                                                        <  5:167807/149‑2 (MQ=255)
          aGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTcgac                                                                                                                                        >  3:325660/1‑149 (MQ=255)
          aGTCTCGTACGCCACACACCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGGCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTcgac                                                                                                                                        <  4:82608/149‑1 (MQ=255)
           gTCTCGTACGCCACACTGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGATCGAATACATCGAGCGTATCATCCACCGCTACCCGTCGAATGCCGCTGCATTcgatg                                                                                                                                       <  5:198287/149‑3 (MQ=255)
           gTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTACCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTcgacg                                                                                                                                       <  4:275383/149‑1 (MQ=255)
              tcGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAg                                                                                                                                     >  4:178428/1‑148 (MQ=255)
                        cacaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCtt                                                                                                                          <  3:378137/149‑1 (MQ=255)
                         acaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCtt                                                                                                                          >  1:138436/1‑148 (MQ=255)
                           aGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAc                                                                                                                       <  2:138436/149‑1 (MQ=255)
                              aaGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAc                                                                                                                       <  1:160671/146‑1 (MQ=255)
                              aaGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAc                                                                                                                       >  2:160671/1‑146 (MQ=255)
                              aaGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTa                                                                                                                    >  8:286413/1‑149 (MQ=255)
                                      cagcaTGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACAACGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAAc                                                                                                            >  4:114291/1‑149 (MQ=255)
                                                    cATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTg                                                                                              >  1:121315/1‑149 (MQ=255)
                                                          tGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTcac                                                                                         >  3:129607/1‑148 (MQ=255)
                                                                    gggAGGCCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACCAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTc                                                                              >  4:113597/1‑149 (MQ=255)
                                                                         ggCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcga                                                                                                                                         <  2:368722/85‑1 (MQ=255)
                                                                         ggCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcga                                                                                                                                         >  1:368722/1‑85 (MQ=255)
                                                                          gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTg                                                                         >  8:2030/1‑148 (MQ=255)
                                                                            ctgcctACGCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCAt                                                                      <  7:45990/149‑1 (MQ=255)
                                                                             tgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGAACGAACTGCTCACACTGCACGCTCAGCTGCAt                                                                      >  3:255151/1‑148 (MQ=255)
                                                                                 tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACt                                                                 >  7:177187/1‑149 (MQ=255)
                                                                                  aCCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTc                                                                >  1:149659/1‑149 (MQ=255)
                                                                                    cccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCgg                                                              >  6:337253/1‑149 (MQ=255)
                                                                                     ccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGc                                                             <  6:37551/149‑1 (MQ=255)
                                                                                        tGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCTGCGTg                                                          >  7:70223/1‑149 (MQ=255)
                                                                                             ttCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAg                                                     >  2:122013/1‑149 (MQ=255)
                                                                                             ttCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAg                                                     >  2:52397/1‑149 (MQ=255)
                                                                                                       tCGAATACATCGAGCGTATCATCTACCGCTACCCATAGAATGACGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCAGGACCACGGCGTGCGCAGCGGGGAtgtg                                           >  2:247925/1‑149 (MQ=255)
                                                                                                          aaTACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCggg                                                 <  8:118512/140‑1 (MQ=255)
                                                                                                          aaTACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCggg                                                 >  7:118512/1‑140 (MQ=255)
                                                                                                                       tATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAAt                               >  1:303341/1‑145 (MQ=255)
                                                                                                                       tATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAAt                               <  2:303341/145‑1 (MQ=255)
                                                                                                                            tcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTg                      >  4:314954/1‑149 (MQ=255)
                                                                                                                            tcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTg                      >  4:136992/1‑149 (MQ=255)
                                                                                                                              cACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGc                     >  3:345919/1‑148 (MQ=255)
                                                                                                                              cACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGc                     <  2:149659/148‑1 (MQ=255)
                                                                                                                                 cGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACt                 <  3:178428/149‑1 (MQ=255)
                                                                                                                                 cGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACt                 >  5:328555/1‑149 (MQ=255)
                                                                                                                                 cGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACt                 <  3:314954/149‑1 (MQ=255)
                                                                                                                                   cTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTgc               >  2:308863/1‑149 (MQ=255)
                                                                                                                                      cccATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTgcgc             <  5:337253/148‑1 (MQ=255)
                                                                                                                                       ccATCGAATTCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGAGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGGGGTTGCGGTGAATGCGACAGTGCAACTGCGCTa           <  1:247925/149‑1 (MQ=255)
                                                                                                                                        cATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTCACCTACAGCGAACTAAGGATCGAAATGATCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACAGCGCTAt          >  1:116637/1‑149 (MQ=255)
                                                                                                                                          tCGAATGCCGCTGCATTCGATGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTgc               <  2:275170/142‑1 (MQ=255)
                                                                                                                                          tCGAATGCCGCTGCATTCGATGAGGACCAGCGCTTGACCTACAGCCAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTgc               >  1:275170/1‑142 (MQ=255)
                                                                                                                                            gAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcg      >  1:301005/1‑149 (MQ=255)
                                                                                                                                              aTGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcgt     >  7:108246/1‑148 (MQ=255)
                                                                                                                                              aTGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcgt     <  8:108246/148‑1 (MQ=255)
                                                                                                                                                gCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcgtcgt  >  6:302026/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGCGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGT  >  NC_002947/4315876‑4316168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑