Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,328,952 G→C 14.7% G67A (GGC→GCC)  PP_3799 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,328,9520GC14.7% 84.7 / 4.9 39G67A (GGC→GCC) PP_3799hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/9);  new base C (3/3);  total (26/13)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTGGCGCACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGCCCAAGTCATTC  >  NC_002947/4328809‑4329091
                                                                                                                                               |                                                                                                                                           
ggCGCTGGCGCACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGc                                                                                                                                        <  8:84636/149‑1 (MQ=255)
  cgcTGGCGCACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCtt                                                                                                                                      <  5:286288/149‑1 (MQ=255)
  cgcTGGCGCACAACCCGATGTGCGAAGGCGAGGAACTGGCCATCGGCCAGGTCATGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCtt                                                                                                                                      <  2:375172/149‑1 (MQ=255)
    cTGGCGCACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTgg                                                                                                                                    <  1:240236/149‑1 (MQ=255)
     tGGCGCACAACCCGATGTGCGAATGCGAGGAACTGCCCAACGGCCAGGTCAGGTCAACGGGCGGTTACTCGGACGCCACCGGTCCCCCCGGGTGGACCGTGGACGTGATGGGTTATGACGACCAGTTGATGGACGGCGGCAGCCTTggg                                                                                                                                   >  6:34376/1‑148 (MQ=255)
         gcACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGcgac                                                                                                                                >  3:127577/1‑148 (MQ=255)
          cacaACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGTCGCCAAGCTTGCcgccga                                                                                                                              <  6:298226/149‑1 (MQ=255)
           acaACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCgacgac                                                                                                                             >  1:20563/1‑149 (MQ=255)
                            tGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTc                                                                                                              >  5:60984/1‑147 (MQ=255)
                               gAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAgcg                                                                                                         >  7:314320/1‑149 (MQ=255)
                                   aaCTGCCCAACGCCCATGTCAAGGGCCCGGGGGGTTTGTCGGCGGGCTGGGGCGCCCCGGGGGTGACCTTGGTCGTGATCGTTTATTCCGAACTGGTGGTGGGGGGCGGAAAGCTTGGCTACGACTCGACGCTGACGTTCAAGCGCCCg                                                                                                     <  4:274898/149‑1 (MQ=255)
                                      tGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGTCGCCAAGc                                                                                                                                        <  1:37173/111‑1 (MQ=255)
                                      tGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGTCGCCAAGc                                                                                                                                        <  1:43025/111‑1 (MQ=255)
                                      tGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGTCGCCAAGc                                                                                                                                        >  2:37173/1‑111 (MQ=255)
                                      tGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGTCGCCAAGc                                                                                                                                        >  2:43025/1‑111 (MQ=255)
                                      tGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGAc                                                                                                  >  7:223473/1‑149 (MQ=255)
                                        gccgttggCCCGGTCATGTGCACGGGCGGTGCCCCGGCCGGCCGCGGGGCCCCGGGGGTGCCCGTGGACGTTATCGTTTATCACCACCCGGGGCGGGTCGGCGTCAAGCTGGGCCACGACTCGACGCTGACGTTCAAGCGCCCGGACgg                                                                                                <  8:285991/143‑1 (MQ=255)
                                         ccACCGGCCAGGTCAAGGGCCCGGGCGGTTTCGCGGTCGCCCGGGGTGCCCCCGGGGTTACCCTGCACGTGATCGTTTATGACTACCAGGTGCTGGTCGGCGGCATGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGc                                                                                               <  8:87731/149‑1 (MQ=255)
                                                 cAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTa                                                                                       >  5:326079/1‑149 (MQ=255)
                                                               cggcggTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATg                                                                         >  7:125485/1‑149 (MQ=255)
                                                                                 cAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTgg                                                       >  6:311928/1‑149 (MQ=255)
                                                                                 cAGCGGTGCCCCCGGGGTGACCCTGGAAGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGCCAAGCTTGGCGACGACTCGACGCTGACCTTCAAGCGCCCGGACGGCGAATACTATGTGCTGTTCGATGCCGGCCCCGGGCACGTgg                                                       >  1:364145/1‑149 (MQ=255)
                                                                                    cGGTGCCCCGGGGGAGACCCTGGCCGGGATCGGTTATCCCGAACCGGGGCGGGCCGGCGGCAAGCTGGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTg                                                    <  4:75374/149‑1 (MQ=255)
                                                                                         ccccgGGGGGGACCGTGGAGGTGATGGTTTATTACGAACCGGTGGGGGGCGGCGGCAAGCGTGCCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTg                                               <  5:34376/149‑1 (MQ=255)
                                                                                          ccccGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTg                                               >  7:28528/1‑148 (MQ=255)
                                                                                                  tGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGcc                                      >  5:384882/1‑149 (MQ=255)
                                                                                                     cccTGGACGTGATCGGTTATGTCGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGCCGTTCCAGCGCCCGGACCGCGAATTGTATGGGCTGTTAGATGCCGGCCCCGGGCACGTGGTTGCAGTGGACTACGCCGa                                    >  2:84643/1‑148 (MQ=255)
                                                                                                       cTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGatat                                 >  3:107028/1‑149 (MQ=255)
                                                                                                        tGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCAc                                                           >  7:340561/1‑122 (MQ=255)
                                                                                                          gACGTGTTCGGTAATGACGAACAGGTGCTGGTCGCCGGAAAGCTTGGCGACCACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGc                              <  2:117791/149‑1 (MQ=255)
                                                                                                           aCGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGcc                             >  4:226568/1‑149 (MQ=255)
                                                                                                                 tCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGTTTGAAGTGGACTACGCCGATATCGCCCAACCa                       >  7:269161/1‑149 (MQ=255)
                                                                                                                     ttATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAg                   >  6:393705/1‑149 (MQ=255)
                                                                                                                     ttATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAg                   >  7:292252/1‑149 (MQ=255)
                                                                                                                     ttATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAg                   >  6:12122/1‑149 (MQ=255)
                                                                                                                            gAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGcc            >  6:323414/1‑149 (MQ=255)
                                                                                                                            gAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGcc            >  1:248841/1‑149 (MQ=255)
                                                                                                                                   tGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGCCCAAGTCa     >  2:125716/1‑149 (MQ=255)
                                                                                                                                      tGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGCCCAAGTCATTc  >  3:334338/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                           
GGCGCTGGCGCACAACCCGATGTGCGAATGCGAGGAACTGGCCAACGGCCAGGTCAAGTGCACCGGCGGTTTCTCGGACGGCAGCGGTGCCCCCGGGGTGACCCTGGACGTGATCGGTTATGACGAACAGGTGCTGGTCGGCGGCAAGCTTGGCGACGACTCGACGCTGACGTTCAAGCGCCCGGACGGCGAATTCTATGTGCTGTTCGATGCCGGCCCCGGGCACGTGGTTGAAGTGGACTACGCCGATATCGCCCAACCATGAGCCGCGCCCAAGTCATTC  >  NC_002947/4328809‑4329091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑