| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,425,441 | G→C | 6.7% | intergenic (‑499/‑301) | PP_5647 ← / → PP_3917 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,425,441 | 0 | G | C | 6.7% | 189.6 / 2.5 | 60 | intergenic (‑499/‑301) | PP_5647/PP_3917 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (28/28); new base C (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
ATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCTGATG > NC_002947/4425299‑4425587 | agctacgcatgctgaatatctgcgatctgttttctctgcgaggtgctgacaggagccggttattgattttttcgtttgtacccgtcggtctgatataaatcttctctagTCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCt < 7:285233‑M1/38‑1 (MQ=255) gtgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCt > 2:192816/1‑148 (MQ=255) tgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTa > 1:99649/1‑148 (MQ=255) aTCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAAtt < 4:135524/148‑1 (MQ=255) tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcattga > 8:349324/1‑141 (MQ=255) tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcattga < 3:14271/146‑6 (MQ=255) tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcattga < 7:349324/146‑6 (MQ=255) tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcattga > 4:14271/1‑141 (MQ=255) tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAAtt > 6:47494/1‑147 (MQ=255) cgtcgCGATCCTCTGGTCTGGCCCTCTTGGATTCATCGTCTGAACGGGTAGGCCAACCGCCATTACGTTCTGACTTGGCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTGAATGCTCAGAAGTCCTGAGCTAAATTGa < 4:172067/148‑1 (MQ=255) gtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAg < 1:3941/148‑1 (MQ=255) tcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAgtg > 2:179858/1‑149 (MQ=255) aTCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTgcgc < 7:173932/149‑1 (MQ=255) tctGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTa > 1:214583/1‑148 (MQ=255) gACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACagag < 5:47494/149‑1 (MQ=255) gggCCCTCTTGGAATGATTGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGAc < 7:342850/148‑1 (MQ=255) ttctctgcgaggtgctgacaggagccggttattgattttttcgtttgtacccgtcggtctgatataaatcttctctagTCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTa > 1:83938‑M1/79‑148 (MQ=255) tctctgcgaggtgctgacaggagccggttattgattttttcgtttgtacccgtcggtctgatataaatcttctctagTCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTag > 8:285233‑M1/78‑148 (MQ=255) ttGGAATCATCGTCTGAACCGTTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGagaa < 6:98284/147‑1 (MQ=255) tGGAATCATCGTCTGAACCGGTAGGACAACTGCGATTACGTTCTTACTTGCCACTTTGTCCGTTATCCCTATCTCTGGTCATACGTTATTCTCAATGCTCTGAAGTCCTTAGCTAAATTGAGTGTGCGCCTAGTGAGACTAGagaaga < 6:158826/148‑1 (MQ=255) gAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGAttt < 1:192816/149‑1 (MQ=255) aaTCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTta < 2:214583/149‑1 (MQ=255) tcGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTatat > 1:325823/1‑147 (MQ=255) tCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTtata < 2:368484/143‑1 (MQ=255) tCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTtata > 1:368484/1‑143 (MQ=255) gAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGa > 2:148861/1‑149 (MQ=255) aaCCGGTAGGCCAACCGCCATTACGATCTCACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATCCTCAGAAGTCCTTAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGAc < 6:20480/149‑1 (MQ=255) aCCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGAc < 6:219691/148‑1 (MQ=255) gCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATCTATATCAGACCGACGGGTACaaa > 4:46141/1‑149 (MQ=255) ccAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAAc < 2:99649/149‑1 (MQ=255) tCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAAcc > 8:5538/1‑149 (MQ=255) aCTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATaa < 1:148861/143‑1 (MQ=255) aCTTGCCACTTTGGCCGTTATCCCTATCTCTGGCCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGc < 3:159328/148‑1 (MQ=255) ttGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATACCCGGCTcc > 8:261307/1‑149 (MQ=255) ttGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTcc > 5:20763/1‑149 (MQ=255) aCTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGATTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCa < 5:108656/149‑1 (MQ=255) aCTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGt > 6:254946/1‑147 (MQ=255) tCCCTATCTCTTGTCATACGCACCTCTCAATGCTCATAAGTCCTTAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCagag < 2:325823/149‑1 (MQ=255) cccTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCagag > 5:19767/1‑148 (MQ=255) TCTCTTGTCATACGCACCTCTCAATGCTCAGAAGTCCTGATCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGaaaa > 1:179858‑M1/1‑147 (MQ=255) CTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGaaaa < 4:114817‑M1/146‑1 (MQ=255) TCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGaaaa < 3:54588‑M1/145‑1 (MQ=255) CTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGa < 8:314543‑M1/148‑1 (MQ=255) tCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCACCTGTAAGCACCTCGCAGAGAAAACACATCg > 6:186018/1‑147 (MQ=255) tCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGAGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGCAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCg > 2:286524/1‑147 (MQ=255) aTACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAg < 7:314543/148‑1 (MQ=255) aCGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTAGAAACGAAAAAATCAATAACAGGCTCCTCTCAGCACCTCGCAGAGAAAACAGATCTCAGata > 3:118701/1‑149 (MQ=255) aCGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCGCCTGTCAGCACATCGCAGAGAAAACAGATCGCAGata > 8:333528/1‑149 (MQ=255) cGCACCTCTCAATTCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGata < 5:186018/148‑1 (MQ=255) aCCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCa < 7:5538/149‑1 (MQ=255) ccTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAg > 4:329186/1‑149 (MQ=255) tctcAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAAcc > 8:16491/1‑101 (MQ=255) tctcAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAAcc < 7:16491/101‑1 (MQ=255) tcAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATCTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCa < 3:46141/147‑1 (MQ=255) aaTGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATAGCAGATATTCAGCATGCg > 7:256620/1‑149 (MQ=255) cTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGc > 7:166765/1‑149 (MQ=255) tCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCt > 7:254679/1‑149 (MQ=255) tCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCt > 8:13038/1‑149 (MQ=255) aGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCTGa < 7:261307/149‑1 (MQ=255) aaGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCTGATg > 4:320968/1‑149 (MQ=255) | ATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGTGTGCGCCTACAGAGACTAGAGAAGATTTATATCAGACCGACGGGTACAAACGAAAAAATCAATAACCGGCTCCTGTCAGCACCTCGCAGAGAAAACAGATCGCAGATATTCAGCATGCGTAGCTGATG > NC_002947/4425299‑4425587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |