| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,450,335 | T→A | 6.3% | C137S (TGC→AGC) | nicA → | nicotinate dehydrogenase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,450,335 | 0 | T | A | 6.3% | 236.7 / 4.2 | 79 | C137S (TGC→AGC) | nicA | nicotinate dehydrogenase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (40/34); new base A (2/3); total (42/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
CGTGCAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCACCCCGC > NC_002947/4450187‑4450481 | cGTGCAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCt > 3:251940/1‑149 (MQ=255) gTGCAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTg > 6:284197/1‑149 (MQ=255) gTGCAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTg > 5:182490/1‑149 (MQ=255) tGCAACAGGCGTTCATCGACTAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGc < 5:393085/149‑1 (MQ=255) cAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCg < 1:224604/148‑1 (MQ=255) aGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCAGCGGCAca < 2:300497/149‑2 (MQ=255) ggCGTTCATCGACGAGCAGGCGGCCCAGTGTGG‑TACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCAGCGGCAcagg < 8:368419/149‑4 (MQ=255) gTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCAGCGGCAcagg < 4:128700/147‑4 (MQ=255) gTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCAGCGGCAcagg > 3:128700/1‑144 (MQ=255) tCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGa > 8:208158/1‑148 (MQ=255) gacgaGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGaaa < 5:355521/148‑1 (MQ=255) acgaGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCTCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATc < 6:182490/149‑1 (MQ=255) acgaGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATc > 4:161249/1‑149 (MQ=255) acgaGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAACGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATc < 1:80912/149‑1 (MQ=255) cgaGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATcc > 8:44485/1‑149 (MQ=255) gCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTgc < 2:30157/149‑1 (MQ=255) ggcggcCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTgcgcg < 3:302829/149‑1 (MQ=255) tACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGc < 7:208158/149‑1 (MQ=255) aCTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGc < 6:330761/148‑1 (MQ=255) cTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCg < 6:259204/149‑1 (MQ=255) tGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCTTTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGAGCTGcc > 1:395697/1‑147 (MQ=255) gCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGcc > 3:44183/1‑146 (MQ=255) cATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGATCAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTCCGCCGCGCTGCCgaga > 7:114012/1‑149 (MQ=255) gtGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGAc > 6:58685/2‑149 (MQ=255) aTGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGAc > 4:148886/1‑149 (MQ=255) aaCGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGAccc < 5:284197/148‑1 (MQ=255) aCGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCg > 2:193228/1‑148 (MQ=255) cGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGga < 6:104606/149‑1 (MQ=255) cGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGga < 5:58685/149‑1 (MQ=255) ggCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCg > 4:342431/1‑146 (MQ=255) aTGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGgaga > 2:357493/1‑147 (MQ=255) aTGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGaaa < 7:44485/149‑1 (MQ=255) tCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAg > 1:338290/1‑146 (MQ=255) tCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCa < 3:161249/149‑1 (MQ=255) tGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATg > 7:218955/1‑148 (MQ=255) aCCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCAGCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAAc < 8:101941/149‑1 (MQ=255) aCCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAAc < 8:218955/149‑1 (MQ=255) cGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCa > 7:219046/1‑149 (MQ=255) gCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCAc < 3:182076/149‑1 (MQ=255) ccAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACt > 3:365790/1‑149 (MQ=255) cAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACt > 3:117205/1‑148 (MQ=255) gCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCacaa < 3:342431/149‑1 (MQ=255) ccTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCacaaca < 4:251940/149‑1 (MQ=255) gATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGATGCGGCACCCACGTCGAAATCCTGCGCGCCGAGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGcc > 7:75803/1‑148 (MQ=255) ttCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCt < 2:346261/79‑1 (MQ=255) ttCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCt > 1:346261/1‑79 (MQ=255) ttCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCcgc > 4:240816/1‑148 (MQ=255) cGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGAcc > 2:90201/1‑148 (MQ=255) cccAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAActg > 6:60589/1‑149 (MQ=255) aGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAActgctg < 1:357493/149‑1 (MQ=255) gCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTgg > 5:360712/1‑149 (MQ=255) cgacgaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGc < 8:219046/149‑1 (MQ=255) acgaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGga > 5:102607/1‑96 (MQ=255) acgaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGga < 6:102607/96‑1 (MQ=255) cgaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCaa < 3:148886/149‑1 (MQ=255) gaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAg > 4:276147/1‑149 (MQ=255) gaACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAg > 1:151569/1‑149 (MQ=255) aaCAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGa > 4:320707/1‑149 (MQ=255) aCAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGa < 2:395697/148‑1 (MQ=255) aCAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGAc > 7:330806/1‑149 (MQ=255) cAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGAcc < 2:338290/149‑1 (MQ=255) ttCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGc < 5:60589/148‑1 (MQ=255) gCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCgtgt > 4:359971/1‑149 (MQ=255) gCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCgtgt > 5:137901/1‑149 (MQ=255) cAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGtt > 2:206428/1‑149 (MQ=255) cAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGtt < 1:193228/149‑1 (MQ=255) aGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGa < 7:7612/149‑1 (MQ=255) gCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGAt > 3:125825/1‑149 (MQ=255) tCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCACCTGCTGGCCAAGACCGGCGTGTTGCTGAtcgtc > 2:190801/1‑149 (MQ=255) tCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGAtcgtc > 5:325393/1‑149 (MQ=255) tCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGAtcgtc < 3:359971/149‑1 (MQ=255) acgtgggtgCCGCAGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCACAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGAc > 1:14208/7‑147 (MQ=255) aCCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAg > 3:352919/1‑148 (MQ=255) ccTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGAc > 1:64557/1‑144 (MQ=255) ccGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCAcccc > 4:95666/1‑149 (MQ=255) ccGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCAcccc < 3:240816/149‑1 (MQ=255) ccGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCAcccc > 2:226214/1‑149 (MQ=255) cGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCACCCCg < 2:64557/149‑1 (MQ=255) gCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCACCCCGc < 3:95666/149‑1 (MQ=255) gCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCCGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCACCCCGc > 1:300638/1‑149 (MQ=255) | CGTGCAACAGGCGTTCATCGACGAGCAGGCGGCCCAGTGTGGTTACTGCATGAACGGCATGATCATGACCGCCAAGGCCCTGCTCGATCGCATTCCCGAGCCCAGCGACGAACAGATTCGCAATGAGCTGTCCGCCAACCTGTGCCGCTGCGGCACCCACGTCGAAATCCTGCGCGCCGTGCGCCGCGCTGCCGAGACCCGGAGAAAGCCATGAACCACTCACAACAGGTGCCCAGCCGCGACCAACTGCTGGCCAAGACCGGCGTGTTGCTGATCGTCGACCAGATCACCCCGC > NC_002947/4450187‑4450481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |