Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,477,817	G→C	6.6%	H59D (CAT→GAT)	PP_3968 ←	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,477,817	0	G	C	6.6%	243.3 / 3.2	80	H59D (CAT→GAT)	PP_3968	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base C (3/2);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCA  >  NC_002947/4477669‑4477960                                                                                                                                                     |                                                                                                                                                gATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATg                                                                                                                                                 >  8:75626/1‑149 (MQ=255)  aTAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATgc                                                                                                                                                >  7:2437/1‑149 (MQ=255)   tAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATgcg                                                                                                                                               >  6:291700/1‑149 (MQ=255)     gAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGTCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCa                                                                                                                                             <  1:330937/149‑1 (MQ=255)      aaCACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAg                                                                                                                                            >  5:317006/1‑149 (MQ=255)         taccGAGCGCTGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                         >  8:228339/4‑149 (MQ=255)          cTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTg                                                                                                                                        <  3:239239/149‑1 (MQ=255)           tCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCa                                                                                                                                             >  5:36925/1‑143 (MQ=255)           tCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCa                                                                                                                                             <  6:36925/143‑1 (MQ=255)             gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGACTGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAg                                                                                                                                      <  1:280052/148‑1 (MQ=255)              agcgcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGAgaa                                                                                                                                    <  8:122364/149‑3 (MQ=255)               gcgcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAg                                                                                                                                   >  6:144256/1‑149 (MQ=255)                 gcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGAGAAggc                                                                                                                                 <  1:27470/149‑3 (MQ=255)                           gTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAg                                                                                                                       >  3:54119/1‑149 (MQ=255)                            tACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGc                                                                                                                      <  8:2437/149‑1 (MQ=255)                            tACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGc                                                                                                                      >  5:314578/1‑149 (MQ=255)                               tCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCgg                                                                                                                    >  8:266592/1‑148 (MQ=255)                                 agagACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCaa                                                                                                                 >  7:43765/1‑149 (MQ=255)                                 agagACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCaa                                                                                                                 >  4:79730/1‑149 (MQ=255)                                 agagACAAATAGATACATCGAAAAGCCCGCTAACAAACGAGTAATCGCATGTGTCTGAGACTACCTATCCCTGCACATTAGACGGCATCAGCCTTGCGCCTTCTCAGACTGCTGAAGCGAACTCAGAGCAGAACGCATATCACAGGCaa                                                                                                                 >  2:159595/1‑149 (MQ=255)                                    gaCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                         >  8:272663/1‑122 (MQ=255)                                    gaCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                         <  7:272663/122‑1 (MQ=255)                                      ccAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTg                                                                                                            >  8:314186/1‑149 (MQ=255)                                        aaTTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCTGTCTGAGCAGTAAGAATATCAGCGGCAACGGTgcg                                                                                                          >  6:284224/1‑149 (MQ=255)                                          ttGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTgcg                                                                                                          >  3:233011/1‑147 (MQ=255)                                            gATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTg                                                                                                                                        >  6:249440/1‑115 (MQ=255)                                            gATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTg                                                                                                                                        <  5:249440/115‑1 (MQ=255)                                               ccATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtc                                                                                                   >  8:300779/1‑149 (MQ=255)                                               acATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGATGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtc                                                                                                   >  3:6265/2‑149 (MQ=255)                                                cATCGAAAAGCCCGCTATCAAACGAGTAATTGCATGGGACTGACCCGACGTATCGCTGCACATTAGAGTGCCACTGACTTTCGACTACTAAGACTCCTGATAAGAACTCTGAGAACTACTCATATCAGCGGCAAAGATACGCAGAAtct                                                                                                  >  5:391086/1‑149 (MQ=255)                                                            cccTATAAAACGAGAAATGGCATCCCTCAGAGCCGCCCTAACGATGCTCAAAAGAGAGCCTCTGCCTTGCGACTTCTCAGACAGCAGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCACACTGAt                                                                                      >  4:288302/3‑149 (MQ=255)                                                              cTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTc                                                                                    <  3:79730/149‑1 (MQ=255)                                                                   aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                               >  5:57842/1‑149 (MQ=255)                                                                   aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                               >  1:244470/1‑149 (MQ=255)                                                                    aaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAgg                                                                              >  3:218040/1‑149 (MQ=255)                                                                    aaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAgg                                                                              >  8:302422/1‑149 (MQ=255)                                                                    aaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTACTGAgg                                                                              >  1:366092/1‑149 (MQ=255)                                                                     aCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGc                                                                             <  7:75626/149‑1 (MQ=255)                                                                        aGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCgg                                                                          <  5:291700/149‑1 (MQ=255)                                                                         gTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGc                                                                         <  7:228339/149‑1 (MQ=255)                                                                          tAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGGCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGc                                                                         <  1:159595/148‑1 (MQ=255)                                                                               gCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATc                                                                   >  5:143858/1‑149 (MQ=255)                                                                                cATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATcc                                                                  >  1:47780/1‑149 (MQ=255)                                                                                cATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATcc                                                                  <  7:302422/149‑1 (MQ=255)                                                                                cATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGATGATGCGCAGTCTGAGCAGTACGCATTTCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATcc                                                                  >  8:5481/1‑149 (MQ=255)                                                                                     gTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGt                                                             >  3:95024/1‑149 (MQ=255)                                                                                      tCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                            <  7:308802/149‑1 (MQ=255)                                                                                       cTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTgg                                                           <  2:47780/149‑1 (MQ=255)                                                                                         gAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTggg                                                          >  1:51141/1‑148 (MQ=255)                                                                                          aGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTtctc                                                                                              <  7:199756/111‑1 (MQ=255)                                                                                          aGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGGGCGCAGAATCTtctc                                                                                              >  8:199756/1‑111 (MQ=255)                                                                                             cgccgTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGt                                                     <  7:300779/149‑1 (MQ=255)                                                                                                      gctgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGa                                            <  8:108122/149‑1 (MQ=255)                                                                                                       ctgctCTTTTGAGGGCCTCTGCCTTGCGGCTTCTCAGACTGCTGATGGGCAGTGTGAGCAGTAGGCATTTGAGGGGCAACGGTGGGGAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCGGTCTTGAc                                           <  3:288302/149‑1 (MQ=255)                                                                                                          ctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATTCTGAGTGGGTAAGTCCGTCTTGACCg                                         >  6:323165/1‑148 (MQ=255)                                                                                                             aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACg                                     >  2:252976/1‑149 (MQ=255)                                                                                                             aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACg                                     >  1:15477/1‑149 (MQ=255)                                                                                                             aTTAGACGGCCTCTGCCTTGAGCCTTCTCATACTGCTTATCCGCACTCTCAGCACTACCCATATCAGCGGCAACGCTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAAACCTGAGTGCTTAAGTCATTCTTGACCGAACg                                     >  4:373928/1‑149 (MQ=255)                                                                                                                agagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATTCTGAGTGGGTAAGTCCGTCTTGACCGAACGTg                                   <  5:323165/148‑1 (MQ=255)                                                                                                                agagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTg                                   <  4:95024/148‑1 (MQ=255)                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  <  2:51141/148‑1 (MQ=255)                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  <  6:317006/148‑1 (MQ=255)                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  <  6:314578/148‑1 (MQ=255)                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  >  6:210367/1‑148 (MQ=255)                                                                                                                 gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                  <  5:180637/148‑1 (MQ=255)                                                                                                                    ggCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGaaa                              >  8:281934/1‑149 (MQ=255)                                                                                                                            ccTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGATCAGTACGAATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctc                         <  5:284224/146‑1 (MQ=255)                                                                                                                            ccTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctcc                        <  1:252976/147‑1 (MQ=255)                                                                                                                             cTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCt                                                                        >  8:92036/1‑98 (MQ=255)                                                                                                                             cTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCt                                                                        <  7:92036/98‑1 (MQ=255)                                                                                                                             cTTGCGCCTTCTCAGACTGCAGAAGCGCAGTCAGAGCACTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTCGGTAAGTCCGTCTTGACCGAACGTGCGAAAtactcac                       >  8:280482/1‑145 (MQ=255)                                                                                                                                 cgcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCt                 <  8:43765/149‑1 (MQ=255)                                                                                                                                    cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGga              <  7:314186/149‑1 (MQ=255)                                                                                                                                    cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGga              <  7:266592/149‑1 (MQ=255)                                                                                                                                    cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGga              <  5:375562/149‑1 (MQ=255)                                                                                                                                        tcAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAAt          >  4:9743/1‑149 (MQ=255)                                                                                                                                        tcAGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAAt          >  6:215872/1‑149 (MQ=255)                                                                                                                                          aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc        >  2:320299/1‑149 (MQ=255)                                                                                                                                          aGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc        >  5:363949/1‑149 (MQ=255)                                                                                                                                             ctgctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTc     <  5:210367/149‑1 (MQ=255)                                                                                                                                                ctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTccc   <  3:9743/148‑1 (MQ=255)                                                                                                                                                ctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTcc    <  4:54119/147‑1 (MQ=255)                                                                                                                                                   aTGCTCTGTCTGAGCATTACGCATTTCATCGGATACTGTTCTCAGAATCTTCTCATAGTGTTTCCTGAGGCCGTCTGATTCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGTGAAATCCTCCCAAGCCTGGAGAATGCTTccct  <  6:391086/146‑2 (MQ=255)                                                                                                                                                     |                                                                                                                                                GATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCA  >  NC_002947/4477669‑4477960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑