Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,520,454 A→T 7.9% intergenic (+45/+27) cspD → / ← icd DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,520,4540AT 7.9% 194.9 / 2.5 68intergenic (+45/+27)cspD/icdDNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/27);  new base T (3/3);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT  >  NC_002947/4520307‑4520600
                                                                                                                                                   |                                                                                                                                                  
ccccAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTa                                                                                                                                                    >  6:335253/1‑148 (MQ=255)
  ccAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaaaa                                                                                                                                                 <  1:114565/149‑1 (MQ=255)
  ccAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaaaa                                                                                                                                                 <  8:96801/149‑1 (MQ=255)
   cAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaa                                                                                                                                                   >  3:319452/1‑146 (MQ=255)
   cAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaa                                                                                                                                                   >  3:197479/1‑146 (MQ=255)
   cAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaa                                                                                                                                                   >  2:31241/1‑146 (MQ=255)
    aaGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAaag                                                                                                                                                >  3:375038/1‑148 (MQ=255)
       ggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGANAAAAAAATCGGCCGCTTCTTTAAAAGAAGc                                                                                                                                            <  1:31241/149‑1 (MQ=255)
       ggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGc                                                                                                                                            <  2:77406/149‑1 (MQ=255)
         gttGCACGCCGTTGAAATCAATGATGCCCCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTTAAAGAAGCgg                                                                                                                                          <  4:265371/148‑2 (MQ=255)
               cGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGtt                                                                                                                                    <  8:48987/149‑1 (MQ=255)
                gCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGttt                                                                                                                                   >  5:338245/1‑149 (MQ=255)
                  cGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTc                                                                                                                                 >  2:272278/1‑149 (MQ=255)
                     tGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCt                                                                                                                                <  7:176704/147‑1 (MQ=255)
                      gAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAACCGGTCGCTTCTTTTAAAGAAGCGGCCGAtttttttg                                                                                                                              <  5:192564/148‑5 (MQ=38)
                       aaaTCCAAGATGCCGCGGCCGATGGGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAATCGAAACTTCAAAAGCGTGACTGCCTGCGCTGTGCCGATAGACAAAAAATTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGtt                                                                                                                             <  8:288632/148‑1 (MQ=255)
                        aaTCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTg                                                                                                                            <  4:375038/148‑1 (MQ=255)
                             aaGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCg                                                                                                                                       <  8:264677/132‑1 (MQ=255)
                             aaGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCg                                                                                                                                       >  7:264677/1‑132 (MQ=255)
                               tctGCCGCAGCGGCCGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCCCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaaaa                                                                                                                                                 <  5:359049/118‑1 (MQ=255)
                               tctGCCGCAGCGGCCGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCCCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaaaa                                                                                                                                                 >  6:359049/3‑120 (MQ=255)
                                  gCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACAtgtg                                                                                                                 >  6:60659/1‑149 (MQ=255)
                                   ccGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGtttt                                                                                                                                  >  2:246879/1‑131 (MQ=255)
                                    cgcgGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGtt                                                                                                               <  3:141543/149‑1 (MQ=255)
                                          cGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTTTAAAGAAGCGGCCGAttttttt                                                                                                                               >  5:88377/1‑124 (MQ=38)
                                          cGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTTTAAAGAAGCGGCCGAttttttt                                                                                                                               <  6:88377/127‑4 (MQ=38)
                                           gCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCAt                                                                                                        >  1:363412/1‑149 (MQ=255)
                                                ggCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCAt                                                                                                   >  6:296501/1‑149 (MQ=255)
                                                           ccAGAGCGTTGTGCGAGCCGCAAAGCGAAACTGCTAATGCCTGTCTGCATGCGCTGTGCCTATAGACAAAAAAATCGGGCGGTTCTTTAAAATTAGCGAGCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCTCCaaatc                                                                                          <  4:319452/147‑3 (MQ=255)
                                                           ccAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCt                                                                                        <  3:344859/149‑1 (MQ=255)
                                                             agagCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGa                                                                                      <  1:272278/149‑1 (MQ=255)
                                                                  cTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAgctgc                                                                                 >  8:72773/1‑149 (MQ=255)
                                                                   ttGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAgctgct                                                                                >  6:247807/1‑149 (MQ=255)
                                                                        agAGCCGCATAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGTTAGACAAAAAACTCGGCCGCTTCTTTAAAAGCAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCa                                                                           <  4:28260/148‑1 (MQ=255)
                                                                              gCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGCCCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTgg                                                                     >  8:269657/1‑149 (MQ=255)
                                                                              gCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTgg                                                                     >  3:165033/1‑149 (MQ=255)
                                                                              gCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCAGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTgg                                                                     >  1:106365/1‑149 (MQ=255)
                                                                               cAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGgc                                                                    >  4:196495/1‑149 (MQ=255)
                                                                                   gCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGccc                                                                >  3:163192/1‑149 (MQ=255)
                                                                                         cTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAACAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATc                                                          <  4:197479/149‑1 (MQ=255)
                                                                                          tGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATc                                                          >  7:91292/1‑148 (MQ=255)
                                                                                          tGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATc                                                          >  5:53333/1‑148 (MQ=255)
                                                                                          tGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATc                                                          >  1:8690/1‑148 (MQ=255)
                                                                                           gCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTTTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCATGAGCTGCTTAACAGCTTGGCGCCCTCCATaaa                                                        >  5:357679/1‑149 (MQ=255)
                                                                                           gCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCaa                                                        >  3:391238/1‑149 (MQ=255)
                                                                                           gCAAAAGCCTGACTCCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCCCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATCTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTAACAGCTTGGCGCCATCCATCa                                                         >  5:363662/1‑148 (MQ=255)
                                                                                            cAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAAt                                                       >  3:40404/1‑149 (MQ=255)
                                                                                             aaaaGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCAtt                                                                                                       <  4:133429/100‑1 (MQ=255)
                                                                                             aaaaGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCAtt                                                                                                       >  3:133429/1‑100 (MQ=255)
                                                                                                    tGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCaa                                                        >  7:262547/1‑140 (MQ=255)
                                                                                                    tGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCaa                                                        <  8:262547/140‑1 (MQ=255)
                                                                                                        tgcctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCg                                           >  5:246910/1‑149 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAgctgc                                                                                 <  3:63048/109‑1 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAgctgc                                                                                 >  4:63048/1‑109 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCg                                           >  3:352218/1‑147 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  7:72773/149‑1 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  6:53333/149‑1 (MQ=255)
                                                                                                           ctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAg                                        >  4:37457/1‑149 (MQ=255)
                                                                                                             gcgcTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCTTCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGt                                      >  3:31886/1‑149 (MQ=255)
                                                                                                             gcgcTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAgg                                       >  4:129886/1‑148 (MQ=255)
                                                                                                                 tgtgCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACg                                  >  8:94518/1‑149 (MQ=255)
                                                                                                                   tgCCGATAGACAAAAAAATCGGCCGCTGCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACg                                  <  2:145912/147‑1 (MQ=255)
                                                                                                                        aTAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTgg                           <  4:40404/149‑1 (MQ=255)
                                                                                                                            aacagaaaaCCGGTCGCTTCTTCTAAAGAAGCGGCCGATTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCg                        >  2:52817/6‑148 (MQ=38)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCaa                     >  2:392954/1‑149 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCaa                     <  2:8690/149‑1 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCaa                     <  5:296501/149‑1 (MQ=255)
                                                                                                                                aaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATg                   >  4:205474/1‑149 (MQ=255)
                                                                                                                                          cGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTgg         >  6:4701/1‑149 (MQ=255)
                                                                                                                                           gCTTCTTTTAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGt        >  3:347954/1‑149 (MQ=255)
                                                                                                                                           gCTTCTTTAACACAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTTCCAGCTTGGCGCCCTCCCTCAATCGCTCGAAATCGTACGTCACGGTCTTGGCCGCAATGGCGCCATTGGt        <  1:121697/149‑1 (MQ=255)
                                                                                                                                           gCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGt        <  3:196495/149‑1 (MQ=255)
                                                                                                                                               cttTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGc      <  5:247807/147‑1 (MQ=255)
                                                                                                                                                tttAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCt   >  7:237873/1‑149 (MQ=255)
                                                                                                                                                  tAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt  <  4:352218/148‑1 (MQ=255)
                                                                                                                                                  tAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt  <  1:154539/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCCAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT  >  NC_002947/4520307‑4520600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑