Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,543,592	C→G	6.4%	S9S (TCC→TCG)	rnz →	ribonuclease Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,543,592	0	C	G	6.4%	195.3 / 4.2	63	S9S (TCC→TCG)	rnz	ribonuclease Z
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

ACACCAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATC  >  NC_002947/4543444‑4543739                                                                                                                                                     |                                                                                                                                                    acacCAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCTGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTcc                                                                                                                                                     >  4:118055/1‑149 (MQ=255) acacCAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGATACAGGAGCCTGAGCATGGCCCTGCTGTTCCTTGGCACCTcc                                                                                                                                                     >  6:22745/1‑149 (MQ=255)  cacCAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccg                                                                                                                                                    >  3:149932/1‑149 (MQ=255)   acCAGCATGCTCATCTGGCGAGAGGAGAACAAGCGGGCGGACGACGGCGGGAGGCATTACGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCAGGCCTGAAACAGGAGCCAGAGCATGGACCTGCTGTTCCTTGGCACCTccgc                                                                                                                                                   >  8:198671/1‑149 (MQ=255)     cAGCAAGCTCATCTGGCGAGAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgcct                                                                                                                                                 >  5:116886/1‑148 (MQ=255)     cAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgccg                                                                                                                                                 >  7:256733/1‑149 (MQ=255)     cAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgccg                                                                                                                                                 >  7:256732/1‑149 (MQ=255)       gCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGGATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCggg                                                                                                                                               >  6:314146/1‑149 (MQ=255)         aaGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGt                                                                                                                                             <  6:241330/149‑1 (MQ=255)               aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGccc                                                                                                                                         <  1:6166/147‑1 (MQ=255)               aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccac                                                                                                                                       >  1:134307/1‑149 (MQ=255)               aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGccaa                                                                                                                                        <  8:356013/148‑3 (MQ=255)               aTCTGGCGACAGGATAACTAGCGCGCGCTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGCCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccac                                                                                                                                       >  3:328722/1‑149 (MQ=255)                    gCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAgg                                                                                                                                  >  2:88279/1‑149 (MQ=255)                      gACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCa                                                                                                                                <  8:256733/149‑1 (MQ=255)                      gACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCa                                                                                                                                <  8:256732/149‑1 (MQ=255)                       aCAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCAc                                                                                                                               <  2:302340/149‑1 (MQ=255)                            aTAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCAGCTTGGCCGCTTCTGGCCTGAAACAGGCGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAAt                                                                                                                          >  7:207397/1‑149 (MQ=255)                                cTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTGGGCACCCCGGCGGAGGTGccaaggaa                                                                                                                                    <  3:229958/135‑7 (MQ=255)                                cTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTGGGCACCCCGGCGGAGGTGccaaggaa                                                                                                                                    >  4:229958/1‑129 (MQ=255)                                   gcgggcggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAgcg                                                                                                                   >  4:206624/1‑149 (MQ=255)                                           tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                   <  3:238415/125‑1 (MQ=255)                                           tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                   >  4:238415/1‑125 (MQ=255)                                              aCGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGt                                                                                                         >  5:196235/1‑148 (MQ=255)                                                ggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGAt                                                                                                      <  6:167504/149‑1 (MQ=255)                                                    ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                  <  3:355930/149‑1 (MQ=255)                                                    ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACACGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGANCGaa                                                                                                  <  5:252477/149‑1 (MQ=255)                                                         cAATCCGGCTACGGTGTGCGCCCCGTATCACCTGGGCCGCTTCTGGCCGGACACCGGAGCCTGAGCAGGGACCTGCTGGGCCTTGGCACCTCCGCCGGGGGGCCCACCACGGCACGCAAGGTCAGCGCCACAGCCGTGATCGAAGCCAg                                                                                             <  3:36800/149‑1 (MQ=255)                                                          aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCgg                                                                                                                                                <  7:332562/97‑1 (MQ=255)                                                          aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCgg                                                                                                                                                >  8:332562/1‑97 (MQ=255)                                                              cGGCTAAGGTGTGCGCCCCGTATCACCTTGGCTGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCa                                                                                        <  3:118055/149‑1 (MQ=255)                                                                    aGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCAc                                                                                   <  5:113204/148‑1 (MQ=255)                                                                      gtgtGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTgg                                                                                >  7:65745/1‑149 (MQ=255)                                                                       tgtgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTgg                                                                                >  6:215666/1‑148 (MQ=255)                                                                         tgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTAt                                                                             <  1:180480/149‑1 (MQ=255)                                                                            gcCCCGTATCCCCTTGGCCGCTTCTGGCCTGAAACCGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                          <  6:396650/149‑1 (MQ=255)                                                                            gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                          <  1:88279/149‑1 (MQ=255)                                                                            gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                          >  7:146552/1‑149 (MQ=255)                                                                            gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGAAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                          >  4:29654/1‑149 (MQ=255)                                                                            gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACAACGGCACGCAATGTCAGCGCCACAGCCGAGATCGAAGCCAGCGGCAGCCACTGGTCTCTg                                                                          >  8:210439/1‑149 (MQ=255)                                                                                  tATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAt                                                                    >  3:123791/1‑149 (MQ=255)                                                                                   aTCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAtt                                                                   >  3:377980/1‑149 (MQ=255)                                                                                         ttGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCTATGTCAGCGCCACAGCCGTGATCGAAGCAAGCTGAAGCCACTGGTATCTGGTCGTATgcggg                                                              >  8:188708/1‑147 (MQ=255)                                                                                         ttGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcggc                                                              >  5:186519/1‑148 (MQ=255)                                                                                           ggCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcggcg                                                             <  4:345580/147‑1 (MQ=255)                                                                                              cGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAgac                                                        <  2:134307/149‑3 (MQ=255)                                                                                              cGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGc                                                        <  4:328722/149‑1 (MQ=255)                                                                                                   cTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAccc                                                    <  8:146552/148‑1 (MQ=255)                                                                                                   cTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAcc                                                     <  6:196235/147‑1 (MQ=255)                                                                                                       ccTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCCCGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCAc                                               >  5:236661/1‑149 (MQ=255)                                                                                                        cTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCTCAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCAc                                               >  2:306053/1‑148 (MQ=255)                                                                                                         tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCg                                             >  4:228449/1‑149 (MQ=255)                                                                                                            aaCAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGcc                                           <  3:206624/148‑1 (MQ=255)                                                                                                               aGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgct                                       <  8:207397/149‑1 (MQ=255)                                                                                                                ggAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgctg                                      <  5:314146/149‑1 (MQ=255)                                                                                                                 gAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCtgctgc                                     >  3:72592/1‑149 (MQ=255)                                                                                                                  aGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGca                                    <  5:215666/149‑1 (MQ=255)                                                                                                                         gCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCg                             >  1:337618/1‑149 (MQ=255)                                                                                                                            tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCTCAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCACCTGCTGCACACCCCGCtt                          <  1:306053/149‑1 (MQ=255)                                                                                                                            tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGTCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCtt                          <  3:29654/149‑1 (MQ=255)                                                                                                                              gACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCATGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCGAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCttt                         <  7:198671/148‑1 (MQ=255)                                                                                                                                  tgctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATc                    >  5:104798/1‑149 (MQ=255)                                                                                                                                      gTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgc                 >  1:40083/1‑148 (MQ=255)                                                                                                                                       ttCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGa               >  7:325292/1‑149 (MQ=255)                                                                                                                                          cTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCt            <  2:40083/149‑1 (MQ=255)                                                                                                                                            gggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCtcc          >  7:84552/2‑147 (MQ=255)                                                                                                                                              gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgctc        >  2:79190/1‑147 (MQ=255)                                                                                                                                              gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgc        <  2:337618/149‑1 (MQ=255)                                                                                                                                              gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcg         <  4:72592/148‑1 (MQ=255)                                                                                                                                                  cTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGa    <  6:186519/149‑1 (MQ=255)                                                                                                                                                    ccgccgGGGTGCCCACCAAAGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATc  >  6:254028/1‑149 (MQ=255)                                                                                                                                                     cgccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCAAAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGAt   <  7:188708/147‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACACCAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATC  >  NC_002947/4543444‑4543739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑