Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,543,595	C→G	6.3%	A10A (GCC→GCG)	rnz →	ribonuclease Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,543,595	0	C	G	6.3%	203.1 / 4.2	63	A10A (GCC→GCG)	rnz	ribonuclease Z
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTTC  >  NC_002947/4543448‑4543742                                                                                                                                                    |                                                                                                                                                    cAGCAAGCTCATCTGGCGAGAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgcct                                                                                                                                                    >  5:116886/1‑148 (MQ=255) cAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgccg                                                                                                                                                    >  7:256733/1‑149 (MQ=255) cAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTccgccg                                                                                                                                                    >  7:256732/1‑149 (MQ=255)   gCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGGATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCggg                                                                                                                                                  >  6:314146/1‑149 (MQ=255)     aaGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGt                                                                                                                                                <  6:241330/149‑1 (MQ=255)           aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGccc                                                                                                                                            <  1:6166/147‑1 (MQ=255)           aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccac                                                                                                                                          >  1:134307/1‑149 (MQ=255)           aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGccaa                                                                                                                                           <  8:356013/148‑3 (MQ=255)           aTCTGGCGACAGGATAACTAGCGCGCGCTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGCCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccac                                                                                                                                          >  3:328722/1‑149 (MQ=255)                gCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAgg                                                                                                                                     >  2:88279/1‑149 (MQ=255)                  gACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCa                                                                                                                                   <  8:256733/149‑1 (MQ=255)                  gACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCa                                                                                                                                   <  8:256732/149‑1 (MQ=255)                   aCAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCAc                                                                                                                                  <  2:302340/149‑1 (MQ=255)                        aTAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCAGCTTGGCCGCTTCTGGCCTGAAACAGGCGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAAt                                                                                                                             >  7:207397/1‑149 (MQ=255)                            cTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTGGGCACCCCGGCGGAGGTGccaaggaa                                                                                                                                       >  4:229958/1‑129 (MQ=255)                            cTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTGGGCACCCCGGCGGAGGTGccaaggaa                                                                                                                                       <  3:229958/135‑7 (MQ=255)                               gcgggcggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAgcg                                                                                                                      >  4:206624/1‑149 (MQ=255)                                       tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                      <  3:238415/125‑1 (MQ=255)                                       tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAg                                                                                                                                      >  4:238415/1‑125 (MQ=255)                                          aCGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGt                                                                                                            >  5:196235/1‑148 (MQ=255)                                            ggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGAt                                                                                                         <  6:167504/149‑1 (MQ=255)                                                ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                     <  3:355930/149‑1 (MQ=255)                                                ggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACACGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGANCGaa                                                                                                     <  5:252477/149‑1 (MQ=255)                                                     cAATCCGGCTACGGTGTGCGCCCCGTATCACCTGGGCCGCTTCTGGCCGGACACCGGAGCCTGAGCAGGGACCTGCTGGGCCTTGGCACCTCCGCCGGGGGGCCCACCACGGCACGCAAGGTCAGCGCCACAGCCGTGATCGAAGCCAg                                                                                                <  3:36800/149‑1 (MQ=255)                                                      aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCgg                                                                                                                                                   <  7:332562/97‑1 (MQ=255)                                                      aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCgg                                                                                                                                                   >  8:332562/1‑97 (MQ=255)                                                          cGGCTAAGGTGTGCGCCCCGTATCACCTTGGCTGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCa                                                                                           <  3:118055/149‑1 (MQ=255)                                                                aGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCAc                                                                                      <  5:113204/148‑1 (MQ=255)                                                                  gtgtGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTgg                                                                                   >  7:65745/1‑149 (MQ=255)                                                                   tgtgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTgg                                                                                   >  6:215666/1‑148 (MQ=255)                                                                     tgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTAt                                                                                <  1:180480/149‑1 (MQ=255)                                                                        gcCCCGTATCCCCTTGGCCGCTTCTGGCCTGAAACCGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                             <  6:396650/149‑1 (MQ=255)                                                                        gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                             <  1:88279/149‑1 (MQ=255)                                                                        gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                             >  7:146552/1‑149 (MQ=255)                                                                        gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGAAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTg                                                                             >  4:29654/1‑149 (MQ=255)                                                                        gcCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACAACGGCACGCAATGTCAGCGCCACAGCCGAGATCGAAGCCAGCGGCAGCCACTGGTCTCTg                                                                             >  8:210439/1‑149 (MQ=255)                                                                              tATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAt                                                                       >  3:123791/1‑149 (MQ=255)                                                                               aTCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGAtt                                                                      >  3:377980/1‑149 (MQ=255)                                                                                     ttGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCTATGTCAGCGCCACAGCCGTGATCGAAGCAAGCTGAAGCCACTGGTATCTGGTCGTATgcggg                                                                 >  8:188708/1‑147 (MQ=255)                                                                                     ttGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcggc                                                                 >  5:186519/1‑148 (MQ=255)                                                                                       ggCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcggcg                                                                <  4:345580/147‑1 (MQ=255)                                                                                          cGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAgac                                                           <  2:134307/149‑3 (MQ=255)                                                                                          cGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGc                                                           <  4:328722/149‑1 (MQ=255)                                                                                               cTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAccc                                                       <  8:146552/148‑1 (MQ=255)                                                                                               cTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAcc                                                        <  6:196235/147‑1 (MQ=255)                                                                                                   ccTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCCCGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCAc                                                  >  5:236661/1‑149 (MQ=255)                                                                                                    cTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCTCAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCAc                                                  >  2:306053/1‑148 (MQ=255)                                                                                                     tGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCg                                                >  4:228449/1‑149 (MQ=255)                                                                                                        aaCAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGcc                                              <  3:206624/148‑1 (MQ=255)                                                                                                           aGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgct                                          <  8:207397/149‑1 (MQ=255)                                                                                                            ggAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgctg                                         <  5:314146/149‑1 (MQ=255)                                                                                                             gAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCtgctgc                                        >  3:72592/1‑149 (MQ=255)                                                                                                              aGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGca                                       <  5:215666/149‑1 (MQ=255)                                                                                                                     gCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCg                                >  1:337618/1‑149 (MQ=255)                                                                                                                        tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCTCAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCACCTGCTGCACACCCCGCtt                             <  1:306053/149‑1 (MQ=255)                                                                                                                        tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGTCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCtt                             <  3:29654/149‑1 (MQ=255)                                                                                                                          gACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCATGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCGAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCttt                            <  7:198671/148‑1 (MQ=255)                                                                                                                              tgctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATc                       >  5:104798/1‑149 (MQ=255)                                                                                                                                  gTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgc                    >  1:40083/1‑148 (MQ=255)                                                                                                                                   ttCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGa                  >  7:325292/1‑149 (MQ=255)                                                                                                                                      cTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCt               <  2:40083/149‑1 (MQ=255)                                                                                                                                        gggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCtcc             >  7:84552/2‑147 (MQ=255)                                                                                                                                          gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgctc           >  2:79190/1‑147 (MQ=255)                                                                                                                                          gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgc           <  2:337618/149‑1 (MQ=255)                                                                                                                                          gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcg            <  4:72592/148‑1 (MQ=255)                                                                                                                                              cTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGa       <  6:186519/149‑1 (MQ=255)                                                                                                                                                ccgccgGGGTGCCCACCAAAGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATc     >  6:254028/1‑149 (MQ=255)                                                                                                                                                 cgccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCAAAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGAt      <  7:188708/147‑1 (MQ=255)                                                                                                                                                   ccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCAGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTtc  <  6:116886/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTTC  >  NC_002947/4543448‑4543742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑