Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,546,047 C→T 7.4% A433A (GCG→GCA)  pydP ← NCS1 family transporter PydP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,546,0470CT 7.4% 156.1 / 2.1 54A433A (GCG→GCA) pydPNCS1 family transporter PydP
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

CCGCCAAGCGCGGCGTACAGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACGCTCTCCAGGCT  >  NC_002947/4545901‑4546193
                                                                                                                                                  |                                                                                                                                                  
ccGCCAAGCGCGGCGTACAGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCAcgc                                                                                                                                                  <  7:66364/149‑1 (MQ=255)
 cGCCAAGCGCGGCGTACAGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCAcgcg                                                                                                                                                 >  7:190840/1‑149 (MQ=255)
         gcggcgTACAGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATacac                                                                                                                                         <  8:190840/149‑1 (MQ=255)
                aCAGTGCACCGCCCAGCAACGAGCCGGGGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGCCGGCAAAACCGGCGCAGTTCCACGCGGGATACACgtcgtc                                                                                                                                   <  4:138826/148‑1 (MQ=255)
                  aGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTcgcg                                                                                                                                <  4:110625/149‑1 (MQ=255)
                  aGTGCACCGCCCAGCAACGAGCCGGGGAACCAGCCACAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTcgcg                                                                                                                                <  3:301736/149‑1 (MQ=255)
                    tGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCgg                                                                                                                               >  5:263659/1‑148 (MQ=255)
                            ccaccaACGCGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCg                                                                                                                      <  8:126407/149‑1 (MQ=255)
                            ccAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCg                                                                                                                      <  6:263659/149‑1 (MQ=255)
                              aGCAACGAGCCGGTGAACCAGCCATAGTCGGAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGc                                                                                                                    <  7:359298/149‑1 (MQ=255)
                                cAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCa                                                                                                                  <  6:322196/149‑1 (MQ=255)
                                  aCGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAgg                                                                                                                <  3:223608/149‑1 (MQ=255)
                                         ggTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCt                                                                                                         >  8:233076/1‑149 (MQ=255)
                                                  gCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGAc                                                                                                >  8:353593/1‑149 (MQ=255)
                                                  gCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGAc                                                                                                <  7:353593/149‑1 (MQ=255)
                                                         tCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAACGCGGCATAACCGGCCCAGTTCCACGCGGGATAAACGTCGTCGAGGTATAGCCCGGCCAGGGCCAGCTGCTGCCGAGGGATCAg                                                                                         >  2:293206/1‑149 (MQ=255)
                                                           gTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAgg                                                                                        <  4:202894/148‑1 (MQ=255)
                                                             aGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCtt                                                                                                        <  8:75709/130‑1 (MQ=255)
                                                             aGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCtt                                                                                                        >  7:75709/1‑130 (MQ=255)
                                                               aaCCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGAt                                                                                            >  3:202057/1‑140 (MQ=255)
                                                               aaCCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGAt                                                                                            <  4:202057/140‑1 (MQ=255)
                                                                 ccAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAagtagt                                                                                 >  5:246563/1‑149 (MQ=255)
                                                                 ccAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAagtagt                                                                                 >  8:8098/1‑149 (MQ=255)
                                                                 ccAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAagtag                                                                                  >  1:259962/1‑148 (MQ=255)
                                                                           ctgctgTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCat                                                                       >  2:231058/1‑149 (MQ=255)
                                                                                 ttGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGcc                                                                 >  2:232630/1‑149 (MQ=255)
                                                                                    ccAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCgg                                                               >  7:347176/1‑148 (MQ=255)
                                                                                       aTCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCaa                                                            >  4:199817/1‑148 (MQ=255)
                                                                                       aTCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCaa                                                            <  3:199817/148‑1 (MQ=255)
                                                                                            cATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCATGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGcc                                                      >  6:58595/1‑149 (MQ=255)
                                                                                            cATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCATGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGcc                                                      >  6:52931/1‑149 (MQ=255)
                                                                                                 ccGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCcaa                                                  >  6:386879/1‑148 (MQ=255)
                                                                                                       gCCCTACAGGCAAGGCGAAGGCGGCAAAACCGTCCCAGTTCCACGCGGGATACACGGCGTCGCGGTATAGCCCGGCCCGGTCCCGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTg                                           <  6:273856/149‑1 (MQ=255)
                                                                                                        cccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGCCGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCACCAAGCTg                                           >  8:293440/1‑148 (MQ=255)
                                                                                                        cccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTgg                                          <  2:259962/149‑1 (MQ=255)
                                                                                                        cccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTg                                           >  7:259755/1‑148 (MQ=255)
                                                                                                         ccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCcaac                                                 >  8:297067/1‑141 (MQ=255)
                                                                                                         ccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCcaac                                                 <  7:297067/141‑1 (MQ=255)
                                                                                                         ccTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTgg                                          >  5:360795/1‑148 (MQ=255)
                                                                                                          cTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGaa                                        <  7:233076/149‑1 (MQ=255)
                                                                                                           tACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGaa                                        >  1:2083/1‑148 (MQ=255)
                                                                                                             cTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCATGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAAt                                       <  5:58595/147‑1 (MQ=255)
                                                                                                             cTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCATGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAAt                                       <  5:52931/147‑1 (MQ=255)
                                                                                                              tGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATa                                      >  2:14368/1‑147 (MQ=255)
                                                                                                              tGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATa                                      <  1:14368/147‑1 (MQ=255)
                                                                                                                gCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGcc                                   <  6:246563/148‑1 (MQ=255)
                                                                                                                    ggcgaaggcgGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGcc                                   >  8:83866/1‑144 (MQ=255)
                                                                                                                    ggcgaaggcgGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGcc                                   <  7:83866/144‑1 (MQ=255)
                                                                                                                            cggcAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTg                      <  1:231058/149‑1 (MQ=255)
                                                                                                                             ggcAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGcc                           <  6:209134/143‑1 (MQ=255)
                                                                                                                             ggcAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGAACAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCaaga                       >  5:209134/1‑144 (MQ=255)
                                                                                                                                  aaCCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATac                <  4:10126/149‑1 (MQ=255)
                                                                                                                                     cGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACg             >  6:181298/1‑149 (MQ=255)
                                                                                                                                     cGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACg             <  1:232630/149‑1 (MQ=255)
                                                                                                                                                cACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACGCTCTCCAGGc   >  1:49573/1‑148 (MQ=255)
                                                                                                                                                 aCGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGTAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACGCTCTCCAGGCt  >  1:253840/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGCCAAGCGCGGCGTACAGTGCACCGCCCAGCAACGAGCCGGTGAACCAGCCATAGTCGTAGAACCAGCTGAAACTGCTGTTGCCAATCGCCATTACCGTCAGCCCTACTGGCAAGGCGAAGGCGGCAAAACCGGCCCAGTTCCACGCGGGATACACGTCGTCGCGGTATAGCCCGGCCAGGTCCAGCTTCTGCCGACGGATCAGGAAGTAGTCCACCACCATGATGCCGGCAATGGGCCCCAACAAGCTGGAATAGCCCAGCAGCCAGTTGGAATACACGCTCTCCAGGCT  >  NC_002947/4545901‑4546193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑