Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,615,343	G→A	7.9%	V572M (GTG→ATG) ‡	PP_4085 →	rhs family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,615,343	0	G	A	7.9%	217.8 / 6.1	76	V572M (GTG→ATG) ‡	PP_4085	rhs family protein
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base A (4/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

ATCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCTCA  >  NC_002947/4615200‑4615491                                                                                                                                                 |                                                                                                                                                      aTCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGAtt                                                                                                                                                  >  3:327272/1‑149 (MQ=255) aTCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTACTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGAtt                                                                                                                                                  >  8:288066/1‑149 (MQ=255)  tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTc                                                                                                                                                 <  6:315074/149‑1 (MQ=255)  tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTc                                                                                                                                                 <  7:90464/149‑1 (MQ=255)  tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTc                                                                                                                                                 <  3:50652/149‑1 (MQ=255)          cTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAATCACTCGCCattcg                                                                                                                                         <  7:304046/149‑5 (MQ=255)           tAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTggg                                                                                                                                        >  8:281296/1‑149 (MQ=255)               ggTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAg                                                                                                                                    <  7:288066/149‑1 (MQ=255)                gTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGc                                                                                                                                   >  2:129369/1‑149 (MQ=255)                   cTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GAt                                                                                                                               >  1:210214/1‑149 (MQ=255)                      tCCGCGAATACGAATGGGCTCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGTGAGTGATTCGCCACTGGGGCAGC‑GATg                                                                                                                              <  7:181612/147‑1 (MQ=255)                      tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCATGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATg                                                                                                                              <  3:351269/147‑1 (MQ=255)                      tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGt                                                                                                                            <  5:258181/149‑1 (MQ=255)                       ccGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCCCTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGt                                                                                                                            >  5:82997/1‑148 (MQ=255)                         gcgAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGaa                                                                                                                         <  6:138660/149‑1 (MQ=255)                          cgAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAATCACTCGCCat                                                                                                                                            <  8:49211/130‑2 (MQ=255)                          cgAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAATCACTCGCCat                                                                                                                                            >  7:49211/1‑129 (MQ=255)                           gAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAaga                                                                                                                       <  7:61217/149‑1 (MQ=255)                            aaTACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAagag                                                                                                                      <  8:238921/149‑1 (MQ=255)                                cGAATGGGCGGGCTTCGCAATGCCTGAAACGCGCCCTACTCCCAAGCGCCGGGACGCAAGGCCTTACCGTTTGCTTCAAGTGCTGTTGACCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTAc                                                                                                                   <  1:309782/149‑1 (MQ=255)                                 gAATGGGCGCGCTTCGCCATTCCTGGAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCg                                                                                                                 >  1:77157/1‑149 (MQ=255)                                 gAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCg                                                                                                                 >  8:351185/1‑149 (MQ=255)                                 gAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCg                                                                                                                 >  5:388168/1‑149 (MQ=255)                                 gAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCg                                                                                                                 >  2:73278/1‑149 (MQ=255)                                       gcgcgcTTCGCCATTCCTGGAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGa                                                                                                           <  2:77157/149‑1 (MQ=255)                                       gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGa                                                                                                           <  6:58559/149‑1 (MQ=255)                                       gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGa                                                                                                           <  6:312860/149‑1 (MQ=255)                                       gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGa                                                                                                           <  2:210214/149‑1 (MQ=255)                                       gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGa                                                                                                           <  4:327272/149‑1 (MQ=255)                                        cgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAg                                                                                                          <  6:82997/149‑1 (MQ=255)                                        cgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAg                                                                                                          <  2:209224/149‑1 (MQ=255)                                        cgcgcTTAGCCATTACTGAAACGCGACCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGCTGAAGAGTACCgctaagag                                                                                                          >  4:122437/1‑142 (MQ=255)                                           gcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCATGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGCTTCGAGTa                                                                                                        >  5:360915/1‑148 (MQ=255)                                             ttCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACaa                                                                                                     >  3:85740/1‑149 (MQ=255)                                                    ttCCTGAAACGCGCCCTACTCCCAAGCGCCTTGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGTGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGaa                                                                                              <  1:56805/149‑1 (MQ=255)                                                    ttCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGt                                                                                                                     >  7:46407/1‑126 (MQ=255)                                                    ttCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGt                                                                                                                     <  8:46407/126‑1 (MQ=255)                                                        tGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCg                                                                                          >  6:203605/1‑149 (MQ=255)                                                          aaaCGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCgg                                                                                         <  6:388168/148‑1 (MQ=255)                                                          aaaCGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGt                                                                                        <  8:258807/149‑1 (MQ=255)                                                                      cTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTc                                                                            <  7:281296/149‑1 (MQ=255)                                                                         ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCAc                                                                          <  7:324114/148‑1 (MQ=255)                                                                         ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg                                                                         >  7:243269/1‑149 (MQ=255)                                                                               gcCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAc                                                                   >  7:354904/1‑149 (MQ=255)                                                                                ccTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAcc                                                                  <  3:320539/149‑1 (MQ=255)                                                                                     aCGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGgc                                                             >  4:115975/1‑149 (MQ=255)                                                                                       ggCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGgccg                                                           >  7:214889/1‑149 (MQ=255)                                                                                         cACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCg                                                               >  4:375525/1‑143 (MQ=255)                                                                                         cACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCg                                                               <  3:375525/143‑1 (MQ=255)                                                                                         cACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGgccgcc                                                         <  2:311247/149‑1 (MQ=255)                                                                                              ccTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGAt                                                    >  7:46446/1‑149 (MQ=255)                                                                                               cTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATc                                                   <  1:129369/149‑1 (MQ=255)                                                                                                   cGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCacta                                               <  4:85740/149‑1 (MQ=255)                                                                                                   cGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCacta                                               >  2:27893/1‑149 (MQ=255)                                                                                                   cGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCacta                                               >  7:38784/1‑149 (MQ=255)                                                                                                          cTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCac                                                 >  4:5755/1‑140 (MQ=255)                                                                                                          cTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCac                                                 <  3:5755/140‑1 (MQ=255)                                                                                                           tCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGt                                                                                        >  2:185997/1‑100 (MQ=255)                                                                                                           tCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGAt                                                    >  2:126365/1‑136 (MQ=255)                                                                                                           tCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑CATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGAt                                                    <  1:126365/136‑1 (MQ=255)                                                                                                             cACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTAGGCCGCCGTGATCACTACTACTggggg                                     >  8:232574/1‑149 (MQ=255)                                                                                                                    ggAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTg                               >  1:294005/1‑149 (MQ=255)                                                                                                                           ccAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAgg                        >  2:304863/1‑149 (MQ=255)                                                                                                                             aaCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGt                       <  5:362721/148‑1 (MQ=255)                                                                                                                             aaCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGt                       >  6:362721/1‑148 (MQ=255)                                                                                                                               cccGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCta                    <  7:351185/149‑1 (MQ=255)                                                                                                                                  gATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCtat                   >  2:293337/1‑147 (MQ=255)                                                                                                                                     ccccagtGGCGAATCACTCGCCATTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGca              >  2:304031/7‑149 (MQ=255)                                                                                                                                      aCGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGca              <  1:73278/148‑1 (MQ=255)                                                                                                                                       ccagtGGCGAATCACTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGcaca            >  3:382560/5‑149 (MQ=255)                                                                                                                                       ccagtGGCGAATCACTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGcaca            >  4:130837/5‑149 (MQ=255)                                                                                                                                       cGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTAc                            >  2:375305/1‑133 (MQ=255)                                                                                                                                       cGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTAc                            <  1:375305/133‑1 (MQ=255)                                                                                                                                          aTGGCGAGTGATTCGCCACTGGGGCAGC‑GATGTTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTc                                                                               >  2:220045/1‑79 (MQ=255)                                                                                                                                          aTGGCGAGTGATTCGCCACTGGGGCAGC‑GATGTTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTc                                                                               <  1:220045/79‑1 (MQ=255)                                                                                                                                                aGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCt    >  7:188065/1‑148 (MQ=255)                                                                                                                                                 gTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCTCa  <  1:27893/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      ATCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAG‑CCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGC‑GATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCTCA  >  NC_002947/4615200‑4615491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑