Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,635,500 C→A 61.9% E86* (GAA→TAA)  uvrY ← BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,635,5000CA61.9% 29.8 / 56.1 50E86* (GAA→TAA) uvrYBarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base C (9/10);  new base A (9/22);  total (18/32)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

AGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCC  >  NC_002947/4635352‑4635642
                                                                                                                                                    |                                                                                                                                              
aGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCACGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTc                                                                                                                                                >  6:245704/1‑149 (MQ=255)
   gcCAACTGCTGGTCGAGCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTc                                                                                                                                             <  8:64145/149‑1 (MQ=255)
   gacAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCGAGACGATTGGCCTGGACCTTTTCGTCAAGGGCTGCACCCTTGGTCATGTAGACGTGAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTc                                                                                                                                             <  5:379105/147‑1 (MQ=255)
       aCTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGcac                                                                                                                                         <  3:154636/149‑1 (MQ=255)
         tgctgGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCacac                                                                                                                                       >  2:280378/1‑149 (MQ=255)
           ctgGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCacac                                                                                                                                       <  7:49891/147‑1 (MQ=255)
                cGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCAcc                                                                                                                                <  5:22838/149‑1 (MQ=255)
                       cGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTa                                                                                                                         >  1:44201/1‑149 (MQ=255)
                              atatAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGCCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGAt                                                                                                                  >  5:31437/1‑149 (MQ=255)
                              atatAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTg                                                                                                                    <  3:221180/147‑1 (MQ=255)
                                 taGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTc                                                                                                               <  1:215811/149‑1 (MQ=255)
                                  aGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGt                                                                                                                >  8:56816/1‑147 (MQ=255)
                                           cGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTg                                                                                                     >  2:297313/1‑149 (MQ=255)
                                                  ggCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagca                                                                                              <  2:232788/149‑1 (MQ=255)
                                                  ggCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagca                                                                                              <  8:197607/149‑1 (MQ=255)
                                                  ggCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCAAACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagca                                                                                              <  1:274494/149‑1 (MQ=255)
                                                     cAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGtt                                                                                           >  8:234540/1‑149 (MQ=255)
                                                      aGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGttt                                                                                          <  1:280378/149‑1 (MQ=255)
                                                          gAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCgg                                                                                      <  5:45024/149‑1 (MQ=255)
                                                            aTGGGCTGGACCATTTCGTCAAGGCCTGCACCCATGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTCTGGGGAACGGGTCGTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTCGCTGCGCAGCAGTTTGCGGGt                                                                                    >  1:182445/1‑149 (MQ=255)
                                                                 cTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCt                                                                               >  6:387031/1‑149 (MQ=255)
                                                                    gACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCa                                                                            <  7:234540/149‑1 (MQ=255)
                                                                    gACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCa                                                                            <  1:14019/149‑1 (MQ=255)
                                                                     aCCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAg                                                                           >  8:313200/1‑149 (MQ=255)
                                                                         tttCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGgccg                                                                       >  5:127408/1‑149 (MQ=255)
                                                                             gTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTGGAGgccaccc                                                                    <  3:227084/148‑2 (MQ=255)
                                                                              tCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGAt                                                                  >  2:51810/1‑149 (MQ=255)
                                                                                  ggCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCg                                                              <  6:144845/149‑1 (MQ=255)
                                                                                   gCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCgg                                                             <  7:313200/149‑1 (MQ=255)
                                                                                   gCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCgg                                                             <  5:387031/149‑1 (MQ=255)
                                                                                           ccTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                     <  6:127408/149‑1 (MQ=255)
                                                                                           ccTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                     <  7:56816/149‑1 (MQ=255)
                                                                                           ccTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCAtttt                                                      >  1:4603/1‑148 (MQ=255)
                                                                                           ccTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGGCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                     <  5:245704/149‑1 (MQ=255)
                                                                                                 tCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCTCTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCAcatcc                                               <  2:172280/149‑1 (MQ=255)
                                                                                                        gCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGca                                        >  7:69750/1‑149 (MQ=255)
                                                                                                         ccGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                     >  7:203944/1‑135 (MQ=255)
                                                                                                         ccGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                     <  8:203944/135‑1 (MQ=255)
                                                                                                                 gcCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGGAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCg                               >  8:191298/1‑149 (MQ=255)
                                                                                                                  ccGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCgg                              <  1:297313/149‑1 (MQ=255)
                                                                                                                      ccTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGTATTTTCACATCCATCAGCACAACGTCCGGTTTc                          <  1:51810/149‑1 (MQ=255)
                                                                                                                      ccTGCAACAAGCGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTc                          <  8:144898/149‑1 (MQ=255)
                                                                                                                                 cGTGTGGGGAACGGGTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGc               <  6:385129/149‑1 (MQ=255)
                                                                                                                                   tgtgGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCa             <  8:69750/149‑1 (MQ=255)
                                                                                                                                         gAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGttt         >  7:313883/1‑147 (MQ=255)
                                                                                                                                             gggTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGc   <  6:31437/149‑1 (MQ=255)
                                                                                                                                             gggTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCAcatccat                                             <  7:46048/107‑1 (MQ=255)
                                                                                                                                             gggTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCAcatccat                                             >  8:46048/1‑107 (MQ=255)
                                                                                                                                              ggTCTTACTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGcc  <  2:4603/149‑1 (MQ=255)
                                                                                                                                               gTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGttt                           <  3:62326/123‑1 (MQ=255)
                                                                                                                                               gTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGttt                           >  4:62326/1‑123 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCC  >  NC_002947/4635352‑4635642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑