Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,666,503 T→C 7.1% M90T (ATG→ACG)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,666,5030TC 7.1% 157.9 / 2.6 56M90T (ATG→ACG) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base T (23/29);  new base C (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTG  >  NC_002947/4666358‑4666648
                                                                                                                                                 |                                                                                                                                                 
gccgccACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCt                                                                                                                                                >  6:235453/1‑149 (MQ=255)
 cctccACGGCCGCCTACGTCATCTTGCAGTTCAACGGCTCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGAGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTc                                                                                                                                               <  4:259224/146‑1 (MQ=255)
 ccgccACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTc                                                                                                                                               <  7:220132/149‑1 (MQ=255)
    ccACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGc                                                                                                                                            <  1:381925/149‑1 (MQ=255)
     cACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCg                                                                                                                                           >  2:355420/1‑149 (MQ=255)
       cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGCACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                         <  1:285816/149‑1 (MQ=255)
       cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                         <  5:337675/149‑1 (MQ=255)
         gccgccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtggt                                                                                                                                       <  4:275464/149‑1 (MQ=255)
            gccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGAc                                                                                                                                    <  2:180225/149‑1 (MQ=255)
               tACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCgg                                                                                                                                 >  8:337154/1‑149 (MQ=255)
                 cGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                               <  8:197425/149‑4 (MQ=255)
                   tCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTTGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                             <  4:217777/149‑1 (MQ=255)
                   tCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                             <  5:235453/149‑1 (MQ=255)
                    cATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCg                                                                                                                            >  2:315774/1‑149 (MQ=255)
                          gCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTc                                                                                                                       <  7:239074/148‑1 (MQ=255)
                            aGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTg                                                                                                                    <  3:124152/149‑1 (MQ=255)
                             gTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGa                                                                                                                   <  1:355420/149‑1 (MQ=255)
                                     tCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTg                                                                                                           >  7:354102/1‑149 (MQ=255)
                                       gCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCGTCCTGATCCACCTGtt                                                                                                         <  7:337154/149‑1 (MQ=255)
                                          ccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCg                                                                                                       >  5:347476/1‑148 (MQ=255)
                                              cTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACACTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATcc                                                                                                  <  5:277103/149‑1 (MQ=255)
                                                gAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                <  3:121064/149‑1 (MQ=255)
                                                 aGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTgg                                                                                               <  1:164427/149‑1 (MQ=255)
                                                    gcggcgCATACAGCCAGCTGCAGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGAGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTa                                                                                             >  4:148757/1‑148 (MQ=255)
                                                          cgTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGAGGGCTTCGCGCCGAACTTCACCCTGTTCCTGTCCGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGt                                                                                      <  5:300509/149‑1 (MQ=255)
                                                               agccagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                  >  6:268185/1‑148 (MQ=255)
                                                                 ccagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAg                                                                               <  6:313667/149‑1 (MQ=255)
                                                                  cagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGc                                                                              <  8:354102/149‑1 (MQ=255)
                                                                   agcTGCCGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                <  1:147545/130‑1 (MQ=255)
                                                                   agcTGCCGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                >  2:147545/1‑130 (MQ=255)
                                                                             gCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTcgc                                                                   >  7:274575/1‑149 (MQ=255)
                                                                               aGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCgcgc                                                                 >  3:295632/1‑149 (MQ=255)
                                                                                  ggATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttc                                                              >  1:236880/1‑149 (MQ=255)
                                                                                     tGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCttcttc                                                           >  6:107009/1‑149 (MQ=255)
                                                                                         ggTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCg                                                        >  8:317130/1‑148 (MQ=255)
                                                                                               cGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACAcc                                                  >  4:161322/1‑148 (MQ=255)
                                                                                                 gCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAAc                                               <  1:111055/149‑1 (MQ=255)
                                                                                                 gCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAAc                                               <  6:347476/149‑1 (MQ=255)
                                                                                                   ttCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCt                                             >  5:396242/1‑149 (MQ=255)
                                                                                                        gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCa                                        >  3:205271/1‑149 (MQ=255)
                                                                                                           gAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCg                                     <  5:268185/149‑1 (MQ=255)
                                                                                                              cTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGc                                    >  7:347731/1‑147 (MQ=255)
                                                                                                                    ccTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGc                            >  4:267029/1‑149 (MQ=255)
                                                                                                                             ggACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                  >  7:166752/1‑86 (MQ=255)
                                                                                                                             ggACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                  <  8:166752/86‑1 (MQ=255)
                                                                                                                              gACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGa                   <  4:295632/148‑1 (MQ=255)
                                                                                                                               aCGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCAGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATc                 >  1:58203/1‑149 (MQ=255)
                                                                                                                                  cgacaccGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTGCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGACTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCtt              >  8:199072/7‑149 (MQ=255)
                                                                                                                                    cTGTCGGTCAGCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTgg            <  5:246191/149‑1 (MQ=255)
                                                                                                                                    cTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGt                         >  6:337514/1‑136 (MQ=255)
                                                                                                                                    cTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGt                         <  5:337514/136‑1 (MQ=255)
                                                                                                                                       tCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTTCTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGa         <  1:315774/149‑1 (MQ=255)
                                                                                                                                        cGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGa         >  4:44994/1‑148 (MQ=255)
                                                                                                                                           tCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAAc     <  7:317130/149‑1 (MQ=255)
                                                                                                                                             aCCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAActc   >  1:336988/1‑147 (MQ=255)
                                                                                                                                              ccATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACctg  <  8:347731/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTG  >  NC_002947/4666358‑4666648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑