Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,666,511 G→A 8.1% V93M (GTG→ATG)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,666,5110GA 8.1% 141.2 / 2.2 49V93M (GTG→ATG) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

CACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTGCTGTTCATCTA  >  NC_002947/4666363‑4666659
                                                                                                                                                    |                                                                                                                                                    
cACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCg                                                                                                                                                      >  2:355420/1‑149 (MQ=255)
  cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGCACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                                    <  1:285816/149‑1 (MQ=255)
  cGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtg                                                                                                                                                    <  5:337675/149‑1 (MQ=255)
    gccgccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCgtggt                                                                                                                                                  <  4:275464/149‑1 (MQ=255)
       gccTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGCCGGTCACCACGCCGAGCATGGTGAc                                                                                                                                               <  2:180225/149‑1 (MQ=255)
          tACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCgg                                                                                                                                            >  8:337154/1‑149 (MQ=255)
            cGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCACGCCGAGCATGGTGACCgaca                                                                                                                                          <  8:197425/149‑4 (MQ=255)
              tCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTTGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                                        <  4:217777/149‑1 (MQ=255)
              tCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTc                                                                                                                                        <  5:235453/149‑1 (MQ=255)
               cATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCg                                                                                                                                       >  2:315774/1‑149 (MQ=255)
                     gCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTc                                                                                                                                  <  7:239074/148‑1 (MQ=255)
                       aGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTg                                                                                                                               <  3:124152/149‑1 (MQ=255)
                        gTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGa                                                                                                                              <  1:355420/149‑1 (MQ=255)
                                tCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTg                                                                                                                      >  7:354102/1‑149 (MQ=255)
                                  gCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCGTCCTGATCCACCTGtt                                                                                                                    <  7:337154/149‑1 (MQ=255)
                                     ccGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCg                                                                                                                  >  5:347476/1‑148 (MQ=255)
                                         cTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACACTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATcc                                                                                                             <  5:277103/149‑1 (MQ=255)
                                           gAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                           <  3:121064/149‑1 (MQ=255)
                                            aGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTgg                                                                                                          <  1:164427/149‑1 (MQ=255)
                                               gcggcgCATACAGCCAGCTGCAGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGAGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTa                                                                                                        >  4:148757/1‑148 (MQ=255)
                                                     cgTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGAGGGCTTCGCGCCGAACTTCACCCTGTTCCTGTCCGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGt                                                                                                 <  5:300509/149‑1 (MQ=255)
                                                          agccagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                             >  6:268185/1‑148 (MQ=255)
                                                            ccagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAg                                                                                          <  6:313667/149‑1 (MQ=255)
                                                             cagcTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGc                                                                                         <  8:354102/149‑1 (MQ=255)
                                                              agcTGCCGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                           <  1:147545/130‑1 (MQ=255)
                                                              agcTGCCGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTg                                                                                                           >  2:147545/1‑130 (MQ=255)
                                                                        gCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTcgc                                                                              >  7:274575/1‑149 (MQ=255)
                                                                          aGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCgcgc                                                                            >  3:295632/1‑149 (MQ=255)
                                                                             ggATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGcttc                                                                         >  1:236880/1‑149 (MQ=255)
                                                                                tGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCttcttc                                                                      >  6:107009/1‑149 (MQ=255)
                                                                                    ggTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCg                                                                   >  8:317130/1‑148 (MQ=255)
                                                                                          cGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACAcc                                                             >  4:161322/1‑148 (MQ=255)
                                                                                            gCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAAc                                                          <  6:347476/149‑1 (MQ=255)
                                                                                            gCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAAc                                                          <  1:111055/149‑1 (MQ=255)
                                                                                              ttCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCt                                                        >  5:396242/1‑149 (MQ=255)
                                                                                                   gcCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCa                                                   >  3:205271/1‑149 (MQ=255)
                                                                                                      gAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCg                                                <  5:268185/149‑1 (MQ=255)
                                                                                                         cTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGc                                               >  7:347731/1‑147 (MQ=255)
                                                                                                               ccTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGc                                       >  4:267029/1‑149 (MQ=255)
                                                                                                                        ggACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                             >  7:166752/1‑86 (MQ=255)
                                                                                                                        ggACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCg                                                                                             <  8:166752/86‑1 (MQ=255)
                                                                                                                         gACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGa                              <  4:295632/148‑1 (MQ=255)
                                                                                                                          aCGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCAGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATc                            >  1:58203/1‑149 (MQ=255)
                                                                                                                             cgacaccGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTGCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGACTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCtt                         >  8:199072/7‑149 (MQ=255)
                                                                                                                               cTGTCGGTCAGCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTgg                       <  5:246191/149‑1 (MQ=255)
                                                                                                                               cTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGt                                    <  5:337514/136‑1 (MQ=255)
                                                                                                                               cTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGt                                    >  6:337514/1‑136 (MQ=255)
                                                                                                                                  tCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTTCTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGa                    <  1:315774/149‑1 (MQ=255)
                                                                                                                                   cGGTCACCACGCCGAGCATGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGa                    >  4:44994/1‑148 (MQ=255)
                                                                                                                                      tCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAAc                <  7:317130/149‑1 (MQ=255)
                                                                                                                                        aCCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAActc              >  1:336988/1‑147 (MQ=255)
                                                                                                                                         ccATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACctg             <  8:347731/149‑1 (MQ=255)
                                                                                                                                                  gCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTGCTGTTCATc    >  4:16627/1‑149 (MQ=255)
                                                                                                                                                    gtggtgACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTGCTGTTCATCTa  >  6:233908/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTTCGCGCCGAACTTCACCCTGTACCTGGACGGCCTGTCGGTCACCATGCTCGGCGTGGTGACCGGTGTCGGCTTCCTGATCCACCTGTTCGCATCCTGGTACATGCGTGGCGAAGCCGGCTACTCGCGCTTCTTCTCGTACACCAACCTGTTCATCGCCAGCATGCTGTTCCTGATCCTTGGCGATAACCTGCTGTTCATCTA  >  NC_002947/4666363‑4666659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑