Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	309,231	T→G	6.2%	P126P (CCT→CCG)	PP_0254 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	309,231	0	T	G	6.2%	196.1 / 3.3	65	P126P (CCT→CCG)	PP_0254	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/35);  new base G (2/2);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CGGACTTGTTGGAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTAGTCGCGCCAGTGGC  >  NC_002947/309083‑309367                                                                                                                                                      |                                                                                                                                         cGGACTTGTTGGAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCt                                                                                                                                          >  5:8402/1‑149 (MQ=255)    aCTTGTTGGAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCtgtg                                                                                                                                       <  5:47669/149‑1 (MQ=255)         ttgGAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGtt                                                                                                                                  >  4:189775/1‑149 (MQ=255)            gAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGc                                                                                                                                <  8:70151/148‑1 (MQ=255)                  gggTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTGGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTCTGGCAACGCCACAGgc                                                                                                                         <  4:1835/149‑1 (MQ=255)                  gggTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGgc                                                                                                                         >  4:336494/1‑149 (MQ=255)                     ttGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCt                                                                                                                      >  4:198146/1‑149 (MQ=255)                        ggAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGc                                                                                                                    <  8:67302/148‑1 (MQ=255)                         gAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAg                                                                                                                  <  5:262178/149‑1 (MQ=255)                           gTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGt                                                                                                                <  2:111818/149‑1 (MQ=255)                               cgcgTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATc                                                                                                            <  7:157672/149‑1 (MQ=255)                                gcgTCTGGCGGGCATGAAGGTATATTTCCGGCTGGCGCACCTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATc                                                                                                            <  1:328983/149‑1 (MQ=255)                                gcgTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGTATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATcc                                                                                                           >  2:101319/1‑149 (MQ=255)                                gcgTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATcc                                                                                                           >  5:150156/1‑149 (MQ=255)                                  gTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAg                                                                                                                  <  1:353499/140‑1 (MQ=255)                                  gTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAg                                                                                                                  >  2:353499/1‑140 (MQ=255)                                  gTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTg                                                                                                         >  5:356343/1‑149 (MQ=255)                                         gggCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGcc                                                                                                  >  1:294505/1‑149 (MQ=255)                                           gcagcaCGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTc                                                                                                <  5:376581/149‑1 (MQ=255)                                            cagcaCGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCg                                                                                               <  6:356343/149‑1 (MQ=255)                                             agcaCGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGt                                                                                              >  1:384931/1‑149 (MQ=255)                                             agcaCGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGt                                                                                              >  4:325981/1‑149 (MQ=255)                                               caCGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGc                                                                                            >  2:232862/1‑149 (MQ=255)                                                 cGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTg                                                                                          <  6:395583/149‑1 (MQ=255)                                                 cGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTg                                                                                          <  3:317846/149‑1 (MQ=255)                                                 cGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGCACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTg                                                                                          <  5:335211/149‑1 (MQ=255)                                                  ggTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTg                                                                                          >  3:12177/1‑148 (MQ=255)                                                   gTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGa                                                                                        <  3:325981/149‑1 (MQ=255)                                                      gagTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGc                                                                                      <  2:39184/148‑1 (MQ=255)                                                         tACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCggca                                                                                  <  4:195948/149‑1 (MQ=255)                                                           ccGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCggcagg                                                                                <  5:111125/149‑1 (MQ=255)                                                           ccGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCggcag                                                                                 <  7:48575/148‑1 (MQ=255)                                                           ccGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCggcag                                                                                 <  3:198146/148‑1 (MQ=255)                                                                tGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATa                                                                            >  5:5483/1‑148 (MQ=255)                                                                  gcgc‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCGGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATaaa                                                                          >  8:165681/1‑148 (MQ=255)                                                                  gcgc‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCGGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATaaa                                                                          >  8:160546/1‑148 (MQ=255)                                                                    gc‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAg                                                                                                                  >  1:259946/1‑106 (MQ=255)                                                                    gc‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAg                                                                                                                  <  2:259946/106‑1 (MQ=255)                                                                    gc‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATaaaaaa                                                                       <  3:189775/149‑1 (MQ=255)                                                                            cGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGc                                                                >  8:260159/1‑149 (MQ=255)                                                                                 gggCGAATCGGACGCATGCGCGGCAGGCATGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTgg                                                             >  7:113337/1‑147 (MQ=255)                                                                                   gCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCa                                                           <  4:378842/147‑1 (MQ=255)                                                                                   gCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAgg                                                         >  4:135054/1‑149 (MQ=255)                                                                                    cGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCGGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAggg                                                        <  7:165681/149‑1 (MQ=255)                                                                                    cGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCGGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAggg                                                        <  7:160546/149‑1 (MQ=255)                                                                                         cGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAggggcggg                                                   >  4:112909/1‑149 (MQ=255)                                                                                          ggACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAggggcgggg                                                  <  6:8402/149‑1 (MQ=255)                                                                                             cGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGTCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtt                                               <  1:101319/149‑1 (MQ=255)                                                                                             cGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtt                                               >  6:142930/1‑149 (MQ=255)                                                                                             cGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtt                                               >  6:136853/1‑149 (MQ=255)                                                                                             cGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtt                                               <  6:5483/149‑1 (MQ=255)                                                                                              gCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCttt                                              <  3:336494/149‑1 (MQ=255)                                                                                               cATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtttt                                             <  2:294505/149‑1 (MQ=255)                                                                                               cATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtttt                                             <  5:54782/149‑1 (MQ=255)                                                                                                  gcgcGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTttgtt                                          >  1:253956/1‑149 (MQ=255)                                                                                                         aggcaCGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCgggg                                   <  7:260159/149‑1 (MQ=255)                                                                                                             aCGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAg                               <  1:232862/149‑1 (MQ=255)                                                                                                                     gCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCt                         >  3:264485/1‑147 (MQ=255)                                                                                                                      cAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAg                      <  6:188579/149‑1 (MQ=255)                                                                                                                        ggTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGc                    <  8:113337/149‑1 (MQ=255)                                                                                                                            ttCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTa                >  5:372289/1‑149 (MQ=255)                                                                                                                            ttCATCGAGCACGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTa                <  5:65817/149‑1 (MQ=255)                                                                                                                              cATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtttt                                             >  3:313241/1‑118 (MQ=255)                                                                                                                              cATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCtttt                                             <  4:313241/118‑1 (MQ=255)                                                                                                                               aTCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTAGTc             >  2:252461/1‑149 (MQ=255)                                                                                                                                       cgcCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTTGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTAGTCGCGCCAGt     <  3:135054/149‑1 (MQ=255)                                                                                                                                          cAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTAGTCGCGCCAGTGgc  >  8:326881/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                         CGGACTTGTTGGAAGTGGGGTTGGGAGTGACGCGTCTGGCGGGCAGCACGGTAGAGTACCGGCTGGCGC‑TGTACCGTCCGGGCGAATCGGACGCATGCGCGGCAGGCACGGTGGTGCAGGTGTTCATCGAGCGCGCCAGTGGCCGGCCTGTGGCGTTGCCAGAGGCGCTGCAGGTGATCCTGTCCGGCCTCGTGCTGGAGCGGCAGGCATAAAAAAGCCCCGCTGGCAGGGGCGGGGCTTTTGTTGCCGGGGGCAGGGCCCTCAGGCGTTAGTCGCGCCAGTGGC  >  NC_002947/309083‑309367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑