Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,742,303 A→G 6.5% V11A (GTG→GCG)  PP_4196 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,742,3030AG 6.5% 176.9 / 2.1 61V11A (GTG→GCG) PP_4196hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base G (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGGCAACCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAACCGA  >  NC_002947/4742158‑4742451
                                                                                                                                                 |                                                                                                                                                    
cTTGGCAACCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGa                                                                                                                                                   <  1:65168/149‑1 (MQ=255)
 ttGGCAACCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGCGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGAt                                                                                                                                                  >  8:216205/1‑149 (MQ=255)
    gCAACCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAg                                                                                                                                               <  3:158346/149‑1 (MQ=255)
      aaCCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGa                                                                                                                                             >  3:40415/1‑149 (MQ=255)
      aaCCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGa                                                                                                                                             >  3:148860/1‑149 (MQ=255)
         cGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCATGGTTTACCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACa                                                                                                                                           >  7:263491/1‑148 (MQ=255)
           cTTTGCTGACTGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAag                                                                                                                                        >  7:14171/1‑149 (MQ=255)
                    ccGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGaa                                                                                                                               <  6:68745/149‑1 (MQ=255)
                     cGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCa                                                                                                                                                <  5:373817/131‑1 (MQ=255)
                     cGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCa                                                                                                                                                >  6:373817/1‑131 (MQ=255)
                      ggCTTGTGGGCAGGCTTGGCAGGCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGc                                                                                                                             <  6:205404/149‑1 (MQ=255)
                            tgtgCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCAt                                                                                                                       <  1:369052/149‑1 (MQ=255)
                            tgtgCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCAt                                                                                                                       <  5:161025/149‑1 (MQ=255)
                            tgtgCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCAt                                                                                                                       <  4:40415/149‑1 (MQ=255)
                            tgtgCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCAt                                                                                                                       <  3:101677/149‑1 (MQ=255)
                                       ggCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGa                                                                                                             >  2:241996/1‑148 (MQ=255)
                                       ggCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCCGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGa                                                                                                             <  4:184110/148‑1 (MQ=255)
                                        gCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACTAAGCAGCGCGAAGCGTTCATGGCAAATGGAc                                                                                                            <  1:241996/148‑1 (MQ=255)
                                            tCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACt                                                                                                       >  4:237805/1‑149 (MQ=255)
                                              gCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTgg                                                                                                     >  4:76026/1‑149 (MQ=255)
                                              gCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTgg                                                                                                     >  3:207872/1‑149 (MQ=255)
                                               ccTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGATTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTgg                                                                                                     >  1:289759/1‑148 (MQ=255)
                                                cTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGAc                                                                                                            >  7:311018/1‑140 (MQ=255)
                                                cTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGAc                                                                                                            <  8:311018/140‑1 (MQ=255)
                                                       ggCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAAt                                                                                                                >  4:275291/1‑129 (MQ=255)
                                                       ggCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAAt                                                                                                                <  3:275291/129‑1 (MQ=255)
                                                        gCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAAt                                                                                           >  2:205273/1‑149 (MQ=255)
                                                          aCGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGc                                                                                         >  6:183385/1‑149 (MQ=255)
                                                            gTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGcc                                                                                        >  7:41100/1‑148 (MQ=255)
                                                                    ccTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGTCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTccc                                                                               <  8:371335/149‑1 (MQ=255)
                                                                    ccTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTccc                                                                               >  4:171711/1‑149 (MQ=255)
                                                                    ccTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTccc                                                                               >  4:194438/1‑149 (MQ=255)
                                                                    ccTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTcc                                                                                >  2:238332/1‑148 (MQ=255)
                                                                    ccTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTcc                                                                                <  1:238332/148‑1 (MQ=255)
                                                                                 aGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAggc                                                                   <  7:216205/148‑1 (MQ=255)
                                                                                   ccTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAggcggc                                                                >  7:266790/1‑149 (MQ=255)
                                                                                   ccTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAggcggc                                                                <  3:237805/149‑1 (MQ=255)
                                                                                    cTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCt                                                               <  1:205273/149‑1 (MQ=255)
                                                                                       gTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCtt                                                              <  3:76026/147‑1 (MQ=255)
                                                                                          tGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCaa                                                          <  4:148860/148‑1 (MQ=255)
                                                                                            ccGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGcc                                                       <  7:301990/149‑1 (MQ=255)
                                                                                            ccGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGc                                                        <  1:349699/148‑1 (MQ=255)
                                                                                             cGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCt                                                      <  3:194438/149‑1 (MQ=255)
                                                                                             cGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCt                                                      <  3:171711/149‑1 (MQ=255)
                                                                                                    gcAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTacaa                                                 >  1:210099/1‑147 (MQ=255)
                                                                                                    gcAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTacaa                                                 >  5:113453/1‑147 (MQ=255)
                                                                                                    gcAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTac                                                   >  1:279666/1‑145 (MQ=255)
                                                                                                    gcAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTac                                                   <  2:279666/145‑1 (MQ=255)
                                                                                                      aGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGc                                             <  2:289759/149‑1 (MQ=255)
                                                                                                       gCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACGCGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCg                                            >  4:152607/1‑149 (MQ=255)
                                                                                                       gCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACGCGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCg                                            >  4:158599/1‑149 (MQ=255)
                                                                                                        ccAAGGCAGTGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACT‑GCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGt                                           >  5:86313/1‑148 (MQ=255)
                                                                                                               aGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCg                                      >  1:262693/1‑147 (MQ=255)
                                                                                                                        cGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTg                           <  8:14171/149‑1 (MQ=255)
                                                                                                                         gggCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGc                          <  8:266790/149‑1 (MQ=255)
                                                                                                                          ggCAGCAGAACGTCAGAAGGCACGCGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGca                         <  3:152607/149‑1 (MQ=255)
                                                                                                                          ggCAGCAGAACGTCAGAAGGCACGCGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGca                         <  3:158599/149‑1 (MQ=255)
                                                                                                                               cagAACGTCAGAAGGCCCACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAAct                    >  5:128499/1‑149 (MQ=255)
                                                                                                                               cagAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTAGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAAct                    >  1:129066/1‑149 (MQ=255)
                                                                                                                                     gTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACt                                                                                                       >  6:247830/1‑60 (MQ=255)
                                                                                                                                     gTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACt                                                                                                       <  5:247830/60‑1 (MQ=255)
                                                                                                                                               acacGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAAcc    >  6:79872/1‑149 (MQ=255)
                                                                                                                                               acacGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAAcc    >  7:79458/1‑149 (MQ=255)
                                                                                                                                               acacGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAAcc    >  6:376062/1‑149 (MQ=255)
                                                                                                                                               acacGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAAcc    >  3:62048/1‑149 (MQ=255)
                                                                                                                                               acacGATCAGGACAAAGCAGCGAGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAAcc    >  7:58075/1‑149 (MQ=255)
                                                                                                                                                cacGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACT‑GCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAACCGa  <  6:86313/149‑1 (MQ=255)
                                                                                                                                                 acGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAACCGa  <  2:262693/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTTGGCAACCGCTTTGCTGACCGGCTTGTGTGCAGGCTTGGCAGTCGCCTTTTTTGGCACGTGCTTACCCTGAGCTGTTTGAGCCTGGTTTGCCGGTTGCGCAGCCAAGGCAGGGACGACCGGGCAGCAGAACGTCAGAAGGCACACGATCAGGACAAAGCAGCGCGAAGCGTTCATGGCAAATGGACAGACTGGCAGGAAAAATGCCTATGCTCCCTGTTCCACAGGCGGCTTGCAAGCCTTACAACAGCGTGACCGCAGTCTCCCTGCACAACTGCTGCGCCAGCAAACCGA  >  NC_002947/4742158‑4742451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑