Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,858,150 G→C 7.8% V67V (GTG→GTC)  PP_4273 → MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,858,1500GC 7.8% 189.8 / 3.4 61V67V (GTG→GTC) PP_4273MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/32);  new base C (2/3);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAATTCCAG  >  NC_002947/4858008‑4858294
                                                                                                                                              |                                                                                                                                                
acGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTg                                                                                                                                            >  5:396633/1‑149 (MQ=255)
 cGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGc                                                                                                                                           <  5:6806/149‑1 (MQ=255)
  gagaAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTg                                                                                                                                            >  3:122039/1‑147 (MQ=255)
  gagaAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTg                                                                                                                                            >  1:342112/1‑147 (MQ=255)
       gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTc                                                                                                                                      >  1:368460/1‑148 (MQ=255)
       gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCg                                                                                                                                     >  5:130831/1‑149 (MQ=255)
         aaGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGAc                                                                                                                                   <  1:354132/149‑1 (MQ=255)
          aGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGAcc                                                                                                                                  <  1:298324/149‑1 (MQ=255)
          aGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGT‑GCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCg                                                                                                                                 <  3:340868/149‑1 (MQ=255)
            gCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGAc                                                                                                                                   <  5:364865/146‑1 (MQ=255)
            gCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGAc                                                                                                                                   >  6:364865/1‑146 (MQ=255)
            gCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCgg                                                                                                                                >  3:60937/1‑149 (MQ=255)
             cTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGc                                                                                                                               <  2:237570/149‑1 (MQ=255)
             cTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTCCACCAGc                                                                                                                               <  2:342112/149‑1 (MQ=255)
              ttGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCgg                                                                                                                                >  8:221993/1‑147 (MQ=255)
                gCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAg                                                                                                                             <  2:248778/148‑1 (MQ=255)
                 cTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTCCACCAGCatc                                                                                                                            <  2:128808/148‑3 (MQ=255)
                   cAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGc                                                                                                                         >  2:186057/1‑149 (MQ=255)
                   cAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGAGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGTGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGc                                                                                                                         >  1:283132/1‑149 (MQ=255)
                    aaGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGc                                                                                                                         <  4:229979/148‑1 (MQ=255)
                    aaGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCt                                                                                                                        >  2:233340/1‑149 (MQ=255)
                    aaGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTcca                                                                                                                                    <  8:248830/137‑3 (MQ=255)
                    aaGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTcca                                                                                                                                    >  7:248830/1‑135 (MQ=255)
                      gTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGa                                                                                                                      <  2:368460/149‑1 (MQ=255)
                                   cgcgGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGa                                                                                                         <  4:171264/149‑1 (MQ=255)
                                      ggCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGAc                                                                                                        >  6:2354/1‑147 (MQ=255)
                                        ccAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGc                                                                                                    <  3:346403/149‑1 (MQ=255)
                                              tCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCAAAGGCGGACGAGCTGGAt                                                                                               >  1:186603/1‑148 (MQ=255)
                                                 aCAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATgcgc                                                                                           <  5:2354/149‑1 (MQ=255)
                                                    gTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGa                                                                                        <  6:244912/149‑1 (MQ=255)
                                                    gTTTGAAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGa                                                                                        >  8:29318/1‑149 (MQ=255)
                                                        gCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGAt                                                                                                                           >  1:113813/1‑110 (MQ=255)
                                                        gCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGAt                                                                                                                           <  2:113813/110‑1 (MQ=255)
                                                          aGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGc                                                                                  >  4:172993/1‑149 (MQ=255)
                                                              gATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGtt                                                                              <  7:221993/149‑1 (MQ=255)
                                                               aTGTGGCAGACCGGGTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGTGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTg                                                                             <  2:283132/149‑1 (MQ=255)
                                                                    gCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGc                                                                        <  2:186603/149‑1 (MQ=255)
                                                                    gCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGc                                                                        <  6:396633/149‑1 (MQ=255)
                                                                      aGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGa                                                                      <  8:102524/149‑1 (MQ=255)
                                                                        aCCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATg                                                                    <  4:122039/149‑1 (MQ=255)
                                                                                  gcTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGt                                                          <  4:60937/149‑1 (MQ=255)
                                                                                  gcTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGt                                                          >  8:151462/1‑149 (MQ=255)
                                                                                    tGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTg                                                        >  8:88339/1‑149 (MQ=255)
                                                                                       tCGCCCTGTGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCgg                                                     <  1:182530/149‑1 (MQ=255)
                                                                                            cTGGGGCAGGCTGCGTGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGc                                                >  6:178285/1‑149 (MQ=255)
                                                                                                     gCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCcgcaacccc                                       <  6:130831/149‑7 (MQ=255)
                                                                                                       tGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGcc                                     >  2:270142/1‑149 (MQ=255)
                                                                                                         cGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCt                                   >  4:380861/1‑149 (MQ=255)
                                                                                                              ttttCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTgaga                               >  3:253884/1‑148 (MQ=255)
                                                                                                                ttCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTgaga                               >  8:144429/1‑146 (MQ=255)
                                                                                                                ttCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGaaa                             <  1:186057/148‑1 (MQ=255)
                                                                                                                ttCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGaaa                             <  1:270142/148‑1 (MQ=255)
                                                                                                                  cGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGa                           <  3:237166/148‑1 (MQ=255)
                                                                                                                   gCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGat                          >  8:87326/1‑148 (MQ=255)
                                                                                                                     tGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGatca                        >  4:204841/1‑148 (MQ=255)
                                                                                                                        aTGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTg                    <  1:233340/149‑1 (MQ=255)
                                                                                                                           aaGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGc                  <  7:29318/148‑1 (MQ=255)
                                                                                                                                    cGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGaaa        <  7:151462/149‑1 (MQ=255)
                                                                                                                                      aTGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAAtt      <  7:87326/149‑1 (MQ=255)
                                                                                                                                       tGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAATTc     <  1:150254/149‑1 (MQ=255)
                                                                                                                                         cTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAATTCca   <  7:144429/149‑1 (MQ=255)
                                                                                                                                          tggtggACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAATTCcag  >  3:161156/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
ACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGCGGACGAGCTGGATGCGCGGATCAAGCGGTTGCAGGCGATGAGCAAGGGGTTGCGGCATGCCGCGCAATGCCCTGAGAAAGATCATCTGGCATGCCCGAAATTCCAG  >  NC_002947/4858008‑4858294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑