Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,930,410 G→C 5.4% T188R (ACA→AGA) ‡ cheZ ← protein phosphatase CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,930,4100GC 5.4% 236.9 / 3.8 74T188R (ACA→AGA) ‡cheZprotein phosphatase CheZ
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base C (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

GACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTC  >  NC_002947/4930267‑4930555
                                                                                                                                                |                                                                                                                                                 
gACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTgtggt                                                                                                                                               >  2:42575/1‑148 (MQ=255)
 aCCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCTGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGa                                                                                                                                             <  2:116377/149‑1 (MQ=255)
  ccTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGAc                                                                                                                                            <  4:277800/149‑1 (MQ=255)
      cACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGAcgc                                                                                                                                          <  2:122690/147‑1 (MQ=255)
      cACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGAcgc                                                                                                                                          <  6:102950/147‑1 (MQ=255)
       aCCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCACACTGGTGAccgagg                                                                                                                                       <  6:319801/149‑6 (MQ=255)
        ccccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCACACTGGTGAccgaggt                                                                                                                                      <  5:164588/149‑7 (MQ=255)
         cccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCtt                                                                                                                                      >  3:79830/1‑148 (MQ=255)
         cccGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCtt                                                                                                                                      >  6:181950/1‑148 (MQ=255)
            gAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATc                                                                                                                                  <  6:4169/149‑1 (MQ=255)
               tCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCAcc                                                                                                                               <  2:775/149‑1 (MQ=255)
                 ggATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCCCCTCGGTCACAAGTGTGGTGAAGCGCTTGATCACCTg                                                                                                                             >  4:217879/1‑149 (MQ=255)
                         ttCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTc                                                                                                                      <  5:297170/148‑1 (MQ=255)
                          tctcGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCa                                                                                                                     <  1:250223/148‑1 (MQ=255)
                            tcGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                                   >  2:283457/1‑148 (MQ=255)
                              gATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCAACTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAgg                                                                                                                   <  1:283457/146‑1 (MQ=255)
                                    tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATa                                                                                                           <  3:217879/148‑1 (MQ=255)
                                        tGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCt                                                                                                      >  7:231871/1‑149 (MQ=255)
                                          cACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTcc                                                                                                       <  5:181950/146‑1 (MQ=255)
                                          cACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                                    <  5:352598/149‑1 (MQ=255)
                                            cGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCAACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  >  5:381203/1‑149 (MQ=255)
                                             gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  >  5:78923/1‑148 (MQ=255)
                                             gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTCAGCAGGTTGCTT‑TCCACCTCGCTCACCAGTGTGGTGACGCGCTTCATCACCTGGCCGGTCAGGTCCTGATAGTCCAGGGc                                                                                                  >  2:197545/1‑148 (MQ=255)
                                               aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  <  4:23749/146‑1 (MQ=255)
                                               aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  >  3:23749/1‑146 (MQ=255)
                                                   gATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaa                                                                                            >  1:242886/1‑148 (MQ=255)
                                                        tGGTCATGTTTTATAGCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGGAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTc                                                                                      <  4:126796/149‑1 (MQ=255)
                                                        tGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTc                                                                                      <  7:170524/149‑1 (MQ=255)
                                                          gTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCg                                                                                     <  4:79830/148‑1 (MQ=255)
                                                             aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaaa                                                                                           <  1:281961/139‑1 (MQ=255)
                                                             aTGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaaa                                                                                           >  2:281961/1‑139 (MQ=255)
                                                               gTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGtt                                                                                   >  7:83963/1‑145 (MQ=255)
                                                               gTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGtt                                                                                   <  8:83963/145‑1 (MQ=255)
                                                                ttttATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCGGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGt                                                                              >  3:32441/1‑149 (MQ=255)
                                                                ttttATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGt                                                                              >  3:27483/1‑149 (MQ=255)
                                                                     taCCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCg                                                                         <  4:185227/149‑1 (MQ=255)
                                                                      aCCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  >  8:318012/1‑123 (MQ=255)
                                                                      aCCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGc                                                                                                  <  7:318012/123‑1 (MQ=255)
                                                                        cGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCAACTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCAGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGa                                                                       >  2:144861/1‑148 (MQ=255)
                                                                         ggCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGaaa                                                                     >  5:183668/1‑149 (MQ=255)
                                                                          gCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTc                                                                                      <  1:259783/131‑1 (MQ=255)
                                                                          gCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTc                                                                                      >  2:259783/1‑131 (MQ=255)
                                                                          gCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAAc                                                                    <  2:242886/149‑1 (MQ=255)
                                                                              aGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTg                                                                >  8:301436/1‑149 (MQ=255)
                                                                                cGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCg                                                              >  2:371252/1‑149 (MQ=255)
                                                                                   tCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGATCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGtt                                                           <  1:42575/149‑1 (MQ=255)
                                                                                          ggctggcCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCg                                                    >  8:191357/1‑149 (MQ=255)
                                                                                           tctggcCATCAGCAACAGCTTGATCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCgg                                                   <  4:27483/148‑1 (MQ=255)
                                                                                           gctggcCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCgg                                                   <  4:32441/149‑1 (MQ=255)
                                                                                             tggcCATCAGCACCAGCTTGAGCAGGTTGCTTGTCCCCCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCCGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGgc                                                  >  1:46796/1‑149 (MQ=255)
                                                                                             tggcCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGgcg                                                 >  6:340860/1‑149 (MQ=255)
                                                                                                ccATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCtg                                              >  5:150756/1‑149 (MQ=255)
                                                                                                       cACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGt                                         >  8:312572/1‑147 (MQ=255)
                                                                                                        aCCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCt                                                      >  1:2998/1‑133 (MQ=255)
                                                                                                        aCCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCt                                                      <  2:2998/133‑1 (MQ=255)
                                                                                                        aCCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAg                                      >  7:168919/1‑149 (MQ=255)
                                                                                                                 aGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGt                              <  6:360876/148‑1 (MQ=255)
                                                                                                                 aGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                             <  1:371252/149‑1 (MQ=255)
                                                                                                                     ggTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                             <  4:105546/145‑1 (MQ=255)
                                                                                                                     ggTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                             >  3:105546/1‑145 (MQ=255)
                                                                                                                           tt‑tCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCtt                    >  3:312334/1‑148 (MQ=255)
                                                                                                                              tCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                             <  7:304070/137‑1 (MQ=255)
                                                                                                                              tCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                             >  8:304070/1‑137 (MQ=255)
                                                                                                                              tCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGa                  <  8:231871/148‑1 (MQ=255)
                                                                                                                               ccACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGTCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                 <  7:312572/148‑1 (MQ=255)
                                                                                                                                 aCCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGt                                         >  1:394672/1‑122 (MQ=255)
                                                                                                                                 aCCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGt                                         <  2:394672/122‑1 (MQ=255)
                                                                                                                                   cTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGa             >  2:219695/1‑148 (MQ=255)
                                                                                                                                     gcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCa          >  4:185125/3‑149 (MQ=255)
                                                                                                                                     gcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCa          >  2:362659/3‑149 (MQ=255)
                                                                                                                                      ggTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATc           <  5:340860/147‑1 (MQ=255)
                                                                                                                                        tCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACg        >  2:372406/1‑148 (MQ=255)
                                                                                                                                            cAGTGTGGTGACGCGCTTGACCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                 >  2:277644/1‑135 (MQ=255)
                                                                                                                                            cAGTGTGGTGACGCGCTTGACCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCCGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                 <  1:277644/135‑1 (MQ=255)
                                                                                                                                             aGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTc  <  6:183668/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTT‑TCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACCTTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATTC  >  NC_002947/4930267‑4930555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑