BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,990,623	C→G	14.2%	S170T (AGC→ACC)	PP_4399 ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,990,623	0	C	G	14.2%	109.7 / 8.4	42	S170T (AGC→ACC)	PP_4399	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (16/20); new base G (4/2); total (20/22)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CTGCCGGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCT  >  NC_002947/4990476‑4990765

cTGCCGGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCt                                                                                                                                               <  4:41448/149‑1 (MQ=255)

    cGGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGaca                                                                                                                                           <  4:374294/149‑1 (MQ=255)

     gggCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGCTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGacac                                                                                                                                          <  2:344515/149‑1 (MQ=255)

              cgcCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGt                                                                                                                                 <  1:81163/149‑1 (MQ=255)

               gcCAGCGGCTTGGCCGTGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTg                                                                                                                                >  1:264581/1‑149 (MQ=255)

                  aGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                             >  2:262870/1‑149 (MQ=255)

                   gCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                             >  5:163530/1‑148 (MQ=255)

                     tggttGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGTGCAGGTGCTTGAAGAGGATGGTCTGGTCGGCCAACAGCAGCGGGTGGCCGTGCTGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGaa                                                                                                                          <  3:235883/146‑1 (MQ=255)

                      gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                         <  6:163530/149‑1 (MQ=255)

                      gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                         <  2:312924/149‑1 (MQ=255)

                          ggCCATGCAGACCACGGTCAGTCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAAGAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                       <  8:172528/147‑1 (MQ=255)

                          ggCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                       <  1:19243/147‑1 (MQ=255)

                            ccATGCAGACCACGGTCAGCCCGTGCTTGAGCGACACTTCCTTGAGCAGGTGCTTTAACAGGACGGTCTGGTCGGCCAACAGAAGCGGGTCGCCTTGCAGCAGGTTGATCTCGACCTGTCTGACACCCATTTCGATCATGAAGGTGTCa                                                                                                                   >  8:298682/1‑149 (MQ=255)

                                 cAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTgg                                                                                                              <  3:343479/149‑1 (MQ=255)

                                     ccACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAg                                                                                                           <  2:201469/148‑1 (MQ=255)

                                      cACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGc                                                                                                         >  3:205736/1‑149 (MQ=255)

                                           tCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAgc                                                                                                    <  2:264581/149‑1 (MQ=255)

                                              gCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGcgcg                                                                                                 >  6:188928/1‑149 (MQ=255)

                                                     cTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATg                                                                                           <  5:188928/148‑1 (MQ=255)

                                                            gcCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGTCATGCAGTGGTa                                                                                   <  1:262870/149‑1 (MQ=255)

                                                                     ttGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCtt                                                                          >  8:32213/1‑149 (MQ=255)

                                                                        aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTga                                                                        <  7:236314/148‑1 (MQ=255)

                                                                        aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCATGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTg                                                                         >  1:295482/1‑147 (MQ=255)

                                                                             gTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGTTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACg                                                                  <  7:298682/149‑1 (MQ=255)

                                                                                         gAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgt                                                      >  6:190078/1‑149 (MQ=255)

                                                                                            ggTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgt                                                   <  4:168758/149‑1 (MQ=255)

                                                                                             gTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgtt                                                  <  7:32213/149‑1 (MQ=255)

                                                                                                tGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACGTCCTTGAAGAACGGGCGCAGGCCGTTGTTgg                                                >  4:367941/1‑148 (MQ=255)

                                                                                                 ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                              >  8:356236/1‑149 (MQ=255)

                                                                                                 ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                              >  6:159875/1‑149 (MQ=255)

                                                                                                  gTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                              <  4:205736/148‑1 (MQ=255)

                                                                                                   tCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                            >  3:279489/1‑149 (MQ=255)

                                                                                                                      cGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGACGGTGGCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATgg                         <  2:184071/149‑1 (MQ=255)

                                                                                                                                   gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc            >  5:6817/1‑149 (MQ=255)

                                                                                                                                   gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc            >  5:1385/1‑149 (MQ=255)

                                                                                                                                      atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                            <  2:200090/109‑1 (MQ=38)

                                                                                                                                      atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                            >  1:200090/6‑114 (MQ=38)

                                                                                                                                        gcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCa       >  5:286264/4‑149 (MQ=255)

                                                                                                                                         cacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCAt      >  8:216686/3‑149 (MQ=255)

                                                                                                                                          acGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATc     >  1:307004/2‑149 (MQ=255)

                                                                                                                                            gAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGc   >  7:177622/1‑149 (MQ=255)

                                                                                                                                             aaatGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCt  <  6:286264/146‑1 (MQ=255)

CTGCCGGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCT  >  NC_002947/4990476‑4990765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`