Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,990,629 C→G 15.4% G168A (GGT→GCT)  PP_4399 ← glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,990,6290CG15.4% 97.2 / 10.3 39G168A (GGT→GCT) PP_4399glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (16/17);  new base G (4/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

GGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAGGCGG  >  NC_002947/4990481‑4990777
                                                                                                                                                    |                                                                                                                                                    
gggCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGCTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGacac                                                                                                                                                      <  2:344515/149‑1 (MQ=255)
         cgcCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGt                                                                                                                                             <  1:81163/149‑1 (MQ=255)
          gcCAGCGGCTTGGCCGTGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTg                                                                                                                                            >  1:264581/1‑149 (MQ=255)
             aGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                                         >  2:262870/1‑149 (MQ=255)
              gCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                                         >  5:163530/1‑148 (MQ=255)
                tggttGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGTGCAGGTGCTTGAAGAGGATGGTCTGGTCGGCCAACAGCAGCGGGTGGCCGTGCTGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGaa                                                                                                                                      <  3:235883/146‑1 (MQ=255)
                 gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                                     <  6:163530/149‑1 (MQ=255)
                 gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                                     <  2:312924/149‑1 (MQ=255)
                     ggCCATGCAGACCACGGTCAGTCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAAGAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                                   <  8:172528/147‑1 (MQ=255)
                     ggCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                                   <  1:19243/147‑1 (MQ=255)
                       ccATGCAGACCACGGTCAGCCCGTGCTTGAGCGACACTTCCTTGAGCAGGTGCTTTAACAGGACGGTCTGGTCGGCCAACAGAAGCGGGTCGCCTTGCAGCAGGTTGATCTCGACCTGTCTGACACCCATTTCGATCATGAAGGTGTCa                                                                                                                               >  8:298682/1‑149 (MQ=255)
                            cAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTgg                                                                                                                          <  3:343479/149‑1 (MQ=255)
                                ccACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAg                                                                                                                       <  2:201469/148‑1 (MQ=255)
                                 cACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGc                                                                                                                     >  3:205736/1‑149 (MQ=255)
                                      tCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAgc                                                                                                                <  2:264581/149‑1 (MQ=255)
                                         gCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGcgcg                                                                                                             >  6:188928/1‑149 (MQ=255)
                                                cTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATg                                                                                                       <  5:188928/148‑1 (MQ=255)
                                                       gcCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGTCATGCAGTGGTa                                                                                               <  1:262870/149‑1 (MQ=255)
                                                                ttGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCtt                                                                                      >  8:32213/1‑149 (MQ=255)
                                                                   aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTga                                                                                    <  7:236314/148‑1 (MQ=255)
                                                                   aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCATGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTg                                                                                     >  1:295482/1‑147 (MQ=255)
                                                                        gTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGTTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACg                                                                              <  7:298682/149‑1 (MQ=255)
                                                                                    gAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgt                                                                  >  6:190078/1‑149 (MQ=255)
                                                                                       ggTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgt                                                               <  4:168758/149‑1 (MQ=255)
                                                                                        gTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgtt                                                              <  7:32213/149‑1 (MQ=255)
                                                                                           tGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACGTCCTTGAAGAACGGGCGCAGGCCGTTGTTgg                                                            >  4:367941/1‑148 (MQ=255)
                                                                                            ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          >  8:356236/1‑149 (MQ=255)
                                                                                            ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          >  6:159875/1‑149 (MQ=255)
                                                                                             gTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          <  4:205736/148‑1 (MQ=255)
                                                                                              tCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        >  3:279489/1‑149 (MQ=255)
                                                                                                                 cGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGACGGTGGCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATgg                                     <  2:184071/149‑1 (MQ=255)
                                                                                                                              gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc                        >  5:1385/1‑149 (MQ=255)
                                                                                                                              gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc                        >  5:6817/1‑149 (MQ=255)
                                                                                                                                 atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        <  2:200090/109‑1 (MQ=38)
                                                                                                                                 atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        >  1:200090/6‑114 (MQ=38)
                                                                                                                                   gcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCa                   >  5:286264/4‑149 (MQ=255)
                                                                                                                                    cacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCAt                  >  8:216686/3‑149 (MQ=255)
                                                                                                                                     acGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATc                 >  1:307004/2‑149 (MQ=255)
                                                                                                                                       gAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGc               >  7:177622/1‑149 (MQ=255)
                                                                                                                                        aaatGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCt              <  6:286264/146‑1 (MQ=255)
                                                                                                                                                   acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGcc           >  7:122215/1‑141 (MQ=255)
                                                                                                                                                   acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGcc           <  8:122215/141‑1 (MQ=255)
                                                                                                                                                   acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAggcg   >  6:31578/1‑149 (MQ=255)
                                                                                                                                                    cccATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAggcgg  <  4:279489/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGCGTACGCGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAGGCGG  >  NC_002947/4990481‑4990777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑