BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,990,633	T→G	13.7%	M167L (ATG→CTG)	PP_4399 ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,990,633	0	T	G	13.7%	102.0 / 11.3	44	M167L (ATG→CTG)	PP_4399	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base T (19/19); new base G (4/2); total (23/21)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

CGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAGGCGG  >  NC_002947/4990490‑4990777

cgcCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGt                                                                                                                                             <  1:81163/149‑1 (MQ=255)

 gcCAGCGGCTTGGCCGTGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTg                                                                                                                                            >  1:264581/1‑149 (MQ=255)

    aGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                                         >  2:262870/1‑149 (MQ=255)

     gCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCAt                                                                                                                                         >  5:163530/1‑148 (MQ=255)

       tggttGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGTGCAGGTGCTTGAAGAGGATGGTCTGGTCGGCCAACAGCAGCGGGTGGCCGTGCTGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGaa                                                                                                                                      <  3:235883/146‑1 (MQ=255)

        gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                                     <  6:163530/149‑1 (MQ=255)

        gCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAg                                                                                                                                     <  2:312924/149‑1 (MQ=255)

            ggCCATGCAGACCACGGTCAGTCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAAGAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                                   <  8:172528/147‑1 (MQ=255)

            ggCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGgt                                                                                                                                   <  1:19243/147‑1 (MQ=255)

              ccATGCAGACCACGGTCAGCCCGTGCTTGAGCGACACTTCCTTGAGCAGGTGCTTTAACAGGACGGTCTGGTCGGCCAACAGAAGCGGGTCGCCTTGCAGCAGGTTGATCTCGACCTGTCTGACACCCATTTCGATCATGAAGGTGTCa                                                                                                                               >  8:298682/1‑149 (MQ=255)

                   cAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTgg                                                                                                                          <  3:343479/149‑1 (MQ=255)

                       ccACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAg                                                                                                                       <  2:201469/148‑1 (MQ=255)

                        cACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGc                                                                                                                     >  3:205736/1‑149 (MQ=255)

                             tCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAgc                                                                                                                <  2:264581/149‑1 (MQ=255)

                                gCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGcgcg                                                                                                             >  6:188928/1‑149 (MQ=255)

                                       cTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATg                                                                                                       <  5:188928/148‑1 (MQ=255)

                                              gcCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGTCATGCAGTGGTa                                                                                               <  1:262870/149‑1 (MQ=255)

                                                       ttGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCtt                                                                                      >  8:32213/1‑149 (MQ=255)

                                                          aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTga                                                                                    <  7:236314/148‑1 (MQ=255)

                                                          aGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCATGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTg                                                                                     >  1:295482/1‑147 (MQ=255)

                                                               gTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGTTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACg                                                                              <  7:298682/149‑1 (MQ=255)

                                                                           gAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgt                                                                  >  6:190078/1‑149 (MQ=255)

                                                                              ggTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgt                                                               <  4:168758/149‑1 (MQ=255)

                                                                               gTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCgttgtt                                                              <  7:32213/149‑1 (MQ=255)

                                                                                  tGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACGTCCTTGAAGAACGGGCGCAGGCCGTTGTTgg                                                            >  4:367941/1‑148 (MQ=255)

                                                                                   ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          >  6:159875/1‑149 (MQ=255)

                                                                                   ggTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          >  8:356236/1‑149 (MQ=255)

                                                                                    gTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGaa                                                          <  4:205736/148‑1 (MQ=255)

                                                                                     tCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        >  3:279489/1‑149 (MQ=255)

                                                                                                        cGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGACGGTGGCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATgg                                     <  2:184071/149‑1 (MQ=255)

                                                                                                                     gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc                        >  5:1385/1‑149 (MQ=255)

                                                                                                                     gTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCgcggc                        >  5:6817/1‑149 (MQ=255)

                                                                                                                        atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        <  2:200090/109‑1 (MQ=38)

                                                                                                                        atgcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACt                                                        >  1:200090/6‑114 (MQ=38)

                                                                                                                          gcacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCa                   >  5:286264/4‑149 (MQ=255)

                                                                                                                           cacGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCAt                  >  8:216686/3‑149 (MQ=255)

                                                                                                                            acGAAATGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATc                 >  1:307004/2‑149 (MQ=255)

                                                                                                                              gAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGc               >  7:177622/1‑149 (MQ=255)

                                                                                                                               aaatGGGTGTCAGCCAGTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCt              <  6:286264/146‑1 (MQ=255)

                                                                                                                                          acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGcc           >  7:122215/1‑141 (MQ=255)

                                                                                                                                          acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGcc           <  8:122215/141‑1 (MQ=255)

                                                                                                                                          acCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAggcg   >  6:31578/1‑149 (MQ=255)

                                                                                                                                           cccATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAggcgg  <  4:279489/149‑1 (MQ=255)

                                                                                                                                            ccATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAggcgg  >  2:384301/1‑148 (MQ=255)

CGCCAGCGGCTTGGCCATGCAGACCACGGTCAGCCCGTGCTTGAGCGCCACTTCCTTGAGCAGGTGCTTGAACAGGAAGGTCTGGTCGGCCAACAGCAGCGGGTCGCCGTGCAGCAGGTTGATCTCGAACTGGCTGACACCCATTTCGTGCATGAAGGTGTCACGTGGCAGGCCGAGCGCGGCCATGCAGTGGTACACCTCCTTGAAGAACGGGCGCAGGCCGTTGTTGGAACTGACACTGAACGCCGAATGGCCCAGCTCGCGGCGGCCATCGCTGCCTACAGGCGG  >  NC_002947/4990490‑4990777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`