Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,041,198 C→T 9.1% intergenic (+176/‑486) PP_5680 → / → PP_4447 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,041,1980CT 9.1% 216.7 / 8.6 77intergenic (+176/‑486)PP_5680/PP_4447Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/32);  new base T (5/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

TTGTACTCCACACCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGT  >  NC_002947/5041052‑5041346
                                                                                                                                                   |                                                                                                                                                     
ttGTACTCCACACCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCt                                                                                                                                                      >  2:43672/1‑148 (MQ=255)
  gTACTCCACACCCAGTCTCACATCTGACGCT‑GTCAGCGACTCTAATGACGGTTTTGATCCAAAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCtt                                                                                                                                                     <  3:159726/147‑1 (MQ=255)
     cTCCACACCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGcc                                                                                                                                                >  5:51526/1‑149 (MQ=255)
       ccACACCCAGTCTCACATCTGACCCTGGTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGccc                                                                                                                                               >  1:17665/1‑149 (MQ=255)
       ccACACCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCccg                                                                                                                                              >  3:191619/1‑149 (MQ=255)
         acacCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCccgcc                                                                                                                                            >  6:8605/1‑149 (MQ=255)
           acCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCccgcc                                                                                                                                            >  2:231315/1‑147 (MQ=255)
            cccAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTC‑TCGGCGGGCTTAAGGCGCGCCgatg                                                                                                                                        <  2:332621/149‑3 (MQ=255)
             ccAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGGGCTTAAGGCGCGCCgat                                                                                                                                         >  7:171640/1‑147 (MQ=255)
             ccAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGGGCTTAAGGCGCGCCgat                                                                                                                                         <  8:171640/148‑2 (MQ=255)
             ccAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCga‑g                                                                                                                                        >  7:68216/1‑148 (MQ=255)
             ccAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGAGGCCAGCACGTCTGCGATGAGGACATGACCTCTTCAGCGGCGCGCCGTAGGGCCGCCga‑g                                                                                                                                        >  6:338546/1‑148 (MQ=255)
                gTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                    <  1:346161/149‑1 (MQ=255)
                gTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                    <  5:8605/149‑1 (MQ=255)
                   tcACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                  >  8:76869/1‑148 (MQ=255)
                    cacaTCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                  >  7:159637/1‑147 (MQ=255)
                     acttCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                  <  7:66040/143‑1 (MQ=255)
                     acaTCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGc                                                                                                                                  >  1:9823/1‑146 (MQ=255)
                            aCCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGAcccc                                                                                                                        <  6:338770/149‑1 (MQ=255)
                            aCCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGAc                                                                                                                           <  8:159637/146‑1 (MQ=255)
                                        gACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAccc                                                                                                             <  1:43672/149‑1 (MQ=255)
                                         aCGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGAcccc                                                                                                            >  6:185502/1‑149 (MQ=255)
                                           gCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCt                                                                                                           >  2:295320/1‑148 (MQ=255)
                                             tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                    <  8:101091/121‑1 (MQ=255)
                                             tAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGa‑gaaga                                                                                                                                    >  7:101091/1‑121 (MQ=255)
                                                        ttGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATgg                                                                                              <  2:17665/148‑1 (MQ=255)
                                                          gATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCg                                                                                            <  1:231315/148‑1 (MQ=255)
                                                              cacaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                       >  3:148854/1‑149 (MQ=255)
                                                               acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                       >  8:279582/1‑148 (MQ=255)
                                                               acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtc                                                                                       >  4:381026/1‑148 (MQ=255)
                                                               acaGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCATAAGGCGGttagg                                                                                      <  6:51526/149‑4 (MQ=255)
                                                                 agagGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTctct                                                                                    <  5:14412/149‑1 (MQ=255)
                                                                     ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtctc                                                                                     >  3:57661/1‑144 (MQ=255)
                                                                     ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTtctc                                                                                     <  4:57661/144‑1 (MQ=255)
                                                                     ggTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCt                                                                                <  8:8261/149‑1 (MQ=255)
                                                                          gggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                            <  6:15971/148‑1 (MQ=255)
                                                                           ggATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGt                                                                          >  4:104252/1‑149 (MQ=255)
                                                                             aTAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTct                                                                        <  7:76869/149‑1 (MQ=255)
                                                                              tAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTctg                                                                       <  8:295038/149‑1 (MQ=255)
                                                                                 ccTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCg                                                                                            >  2:174416/1‑125 (MQ=255)
                                                                                   ttAAGGCGTAATTGTCTGATGCCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCtgctgc                                                                   >  3:375534/1‑148 (MQ=255)
                                                                                      aGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAg                                                               <  2:9823/149‑1 (MQ=255)
                                                                                        gCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                            >  7:307894/1‑134 (MQ=255)
                                                                                        gCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCa                                                                            <  8:307894/134‑1 (MQ=255)
                                                                                        gCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGc                                                             >  2:388435/1‑149 (MQ=255)
                                                                                             aTTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATa                                                        <  7:369898/149‑1 (MQ=255)
                                                                                              ttGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAg                                                       >  1:392942/1‑149 (MQ=255)
                                                                                                   tGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGCAGGCCGATAGCCGAg                                                  >  6:271489/1‑149 (MQ=255)
                                                                                                     aTACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGCCCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTc                                                >  4:68197/1‑149 (MQ=255)
                                                                                                         cAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCTCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc                                                     <  4:218338/140‑1 (MQ=255)
                                                                                                         cAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCTCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGcc                                                     >  3:218338/1‑140 (MQ=255)
                                                                                                         cAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaac                                            <  7:201803/149‑1 (MQ=255)
                                                                                                           acacCTCTGCGATGAGGACATGACCTCTTCACCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTccaacc                                           >  3:19284/1‑148 (MQ=255)
                                                                                                               ctctGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGCTAGCCGAGGCCCACCAATTc                                      >  1:182225/1‑149 (MQ=255)
                                                                                                                    cGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCTGATAGCCGAGTCCAACCAATTCTAt                                   <  5:338546/147‑1 (MQ=255)
                                                                                                                    cGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTAtt                                  <  3:104252/148‑1 (MQ=255)
                                                                                                                      aTGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTAttttt                               >  1:217594/1‑149 (MQ=255)
                                                                                                                      aTGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTAttttt                               >  3:72780/1‑149 (MQ=255)
                                                                                                                       tGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTc                              <  2:217594/149‑1 (MQ=255)
                                                                                                                       tGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTc                              >  3:243418/1‑149 (MQ=255)
                                                                                                                         aGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCg                             <  5:185502/148‑1 (MQ=255)
                                                                                                                         aGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCg                             <  1:295320/148‑1 (MQ=255)
                                                                                                                         aGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGa                            <  4:375534/149‑1 (MQ=255)
                                                                                                                               tGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGa                                                   >  4:335551/1‑120 (MQ=255)
                                                                                                                               tGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGc                      <  2:392942/149‑1 (MQ=255)
                                                                                                                                tggctctTC‑TCGGCGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGcc                     >  8:249695/4‑149 (MQ=38)
                                                                                                                                 aCCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTg                        >  6:236529/1‑145 (MQ=255)
                                                                                                                                 aCCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTg                        <  5:236529/145‑1 (MQ=255)
                                                                                                                                  ccTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCt                    >  4:103081/1‑148 (MQ=255)
                                                                                                                                   ctctTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCACCCAATAATATTTTTCGACCTGGCCTGa                  >  6:30807/1‑149 (MQ=255)
                                                                                                                                    tctTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGAc                 >  3:361985/1‑149 (MQ=255)
                                                                                                                                       ttctCGGCGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCg               >  7:360367/4‑149 (MQ=255)
                                                                                                                                        tctCGGCGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCg               >  3:118277/3‑148 (MQ=255)
                                                                                                                                        tctCGGCGGGCTTAAGGCGCGCCGATGAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCg               >  3:112802/3‑148 (MQ=255)
                                                                                                                                          tCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGt           <  8:68216/149‑1 (MQ=255)
                                                                                                                                             gcgcgcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTaga        <  3:381026/149‑1 (MQ=255)
                                                                                                                                               gcgcCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGc      >  3:152855/1‑149 (MQ=255)
                                                                                                                                                   cTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCgtgt  >  2:391848/1‑149 (MQ=255)
                                                                                                                                                   cTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCgtgt  >  5:397350/1‑149 (MQ=255)
                                                                                                                                                   cTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTATCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCgagt  >  4:171660/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTGTACTCCACACCCAGTCTCACATCTGACCCT‑GTCAGCGACGCTAATGACGGTGTTGATCCACAGAGGGTTAGGGATAGCCTTAAGGCGTAATTGTCTGATACCAACACCTCTGCGATGAGGACATGACCTCTTCATCGGCGCGCCTTAAGCCCGCCGA‑GAAGAGCCAGAGACCCCGAGCAAGACCCCTAACCGCCTTATGGCGGTTTCTCTGCCTCCCAGTCTGCTGCGAAGGCCGATAGCCGAGTCCAACCAATTCTATTTTTCGACCTGGCCTGACCGCAGTAGAGCGTGT  >  NC_002947/5041052‑5041346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑