| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,059,874 | G→T | 9.5% | G320G (GGC→GGA) | PP_4458 ← | opine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,059,874 | 0 | G | T | 9.5% | 169.4 / 2.9 | 56 | G320G (GGC→GGA) | PP_4458 | opine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (29/21); new base T (2/4); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTGGAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTCCA > NC_002947/5059732‑5060022 | cgTTGGAACCAACCGATTTGAGTTTTAACCCGCTGGGATAACCCGCGCTGGCCAACAAGGCGTTCGCCTGCGCAGGATCGTACGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGCGGATGC‑‑‑TCCATCa < 4:121681/149‑1 (MQ=255) gTTGGAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAg > 2:169517/1‑149 (MQ=255) ggAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTa > 2:122123/1‑149 (MQ=255) gAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTa > 7:4602/1‑148 (MQ=255) gAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTa > 1:61299/1‑148 (MQ=255) acCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCttt < 4:148715/149‑1 (MQ=255) ccGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGCGGATGC‑‑‑TCCATCatac < 3:263210/140‑4 (MQ=255) ccGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGCGGATGC‑‑‑TCCATCatac > 4:263210/1‑137 (MQ=255) aTTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTAccc < 4:99540/149‑1 (MQ=255) aGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTa < 1:255470/149‑1 (MQ=255) gcccGTTGGGATAAACCGCGCTGGACAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATAGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCACCGAACCGAAt > 2:306772/2‑148 (MQ=255) aCCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAAt > 7:18440/1‑148 (MQ=255) cccGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAg < 8:117119/142‑1 (MQ=255) cccGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAg > 7:117119/1‑142 (MQ=255) cGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGAtc < 8:4602/149‑1 (MQ=255) aCCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATg < 4:226937/148‑1 (MQ=255) cccGCGCTGGCCAAGAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCATTCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGtt < 5:288624/149‑1 (MQ=255) cccGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGCGGATGC‑‑‑TCCATCatac < 5:266436/111‑4 (MQ=255) cccGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGCGGATGC‑‑‑TCCATCatac > 6:266436/1‑108 (MQ=255) ccGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGtt > 3:290778/1‑148 (MQ=255) gcgcTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGAc < 7:10208/149‑1 (MQ=255) tttCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCtt > 7:229886/1‑149 (MQ=255) cgTGCGCAGGATCGTTAGCGTATTGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCCCTTTCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGc < 1:122123/149‑1 (MQ=255) gcAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGAGCTCATTGATGGTGACCGACGCAGCGATAGCGTGCCGAACCtt > 4:305916/1‑149 (MQ=255) tgATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGAc < 1:367298/148‑1 (MQ=255) ggATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGAc < 1:361565/149‑1 (MQ=255) ggATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGAc < 2:61299/149‑1 (MQ=255) gATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGATACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGAcg > 5:207816/1‑149 (MQ=255) tCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGAcgtc > 1:197160/1‑149 (MQ=255) tAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCATAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACgtcgtc > 4:383046/1‑149 (MQ=255) aTGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGCCAGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTACCGGc > 2:248243/1‑149 (MQ=255) gCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCtt < 2:197160/148‑1 (MQ=255) cccGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACGTCTTTACCCGCGTAGCGAATGATCTCATTGATGTTTACCGACGCAGCGATAGCTTGc < 2:200801/121‑1 (MQ=255) cccGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACGTCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGc > 1:200801/1‑121 (MQ=255) ccGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGa > 2:282927/1‑148 (MQ=255) ccGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGa > 5:77345/1‑148 (MQ=255) ccGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGa > 7:255338/1‑148 (MQ=255) cTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCg < 1:185828/149‑1 (MQ=255) gCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGt < 8:229886/149‑1 (MQ=255) cAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTaag > 6:356054/1‑148 (MQ=255) ccccTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTaagaaaga < 8:18440/149‑1 (MQ=255) ccTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTaagaaaga > 2:45507/1‑147 (MQ=255) ccTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTaagaaaga > 7:16083/1‑147 (MQ=255) ccTGGTAGCCATTGGGTATGATGGAGAATCC‑‑‑GCCATCAGCTACATCTTTACCCTCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAagaga < 3:333568/149‑1 (MQ=255) cTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAagagag > 6:11206/1‑149 (MQ=255) cTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACAGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTaagaaaga > 5:33844/1‑146 (MQ=255) tAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACg > 5:123722/1‑149 (MQ=255) aGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGATTGATATCATTGATGTTGACCGACGCAGAGATAGCTTTCCGAACCTTGACGTCGTATAACGTCTTGATATTCAGGTTAAGAAATAGATTAct > 5:352134/1‑147 (MQ=255) ggTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGGGTACGGAATTCgg > 7:91462/1‑148 (MQ=255) ggTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCgg > 3:2924/1‑148 (MQ=255) gTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCa < 5:11206/149‑1 (MQ=255) tATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAg > 2:176569/1‑149 (MQ=255) tATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAg < 7:265067/149‑1 (MQ=255) tATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAg > 5:198080/1‑149 (MQ=255) tgatgGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGc < 8:16083/149‑1 (MQ=255) c‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTcc < 4:2924/149‑1 (MQ=255) gCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACTGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTCCa < 1:169517/149‑1 (MQ=255) gCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTCCa > 6:338683/1‑149 (MQ=255) gCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTCCa < 4:290778/149‑1 (MQ=255) | CGTTGGAAACAACCGATTTGAGTTTTAACCCGTTGGGATAACCCGCGCTGGCCAACAAGGCTTTCGCGTGCGCAGGATCGTAAGCGTATGGCCCGGCACTGCAATCAAGCCCCTGGTAGCCATTGGGTATGATGGAGCATCC‑‑‑GCCATCAGCTACATCTTTACCCGCGTAGCGAATGATCTCATTGATGTTGACCGACGCAGCGATAGCTTGCCGAACCTTGACGTCGTCTAACGGCTTGATATTCCGGTTAAGAAAGAGAGTACGGAATTCGGCAGGCTCAAAAACGTCCA > NC_002947/5059732‑5060022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |