Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,074,529 C→A 5.4% R12L (CGC→CTC)  PP_5692 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,074,5290CA 5.4% 261.5 / 3.7 84R12L (CGC→CTC) PP_5692hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/37);  new base A (3/2);  total (45/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

GTTAGGTTCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGA  >  NC_002947/5074382‑5074677
                                                                                                                                                   |                                                                                                                                                      
gTTAGGTTCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTcgcg                                                                                                                                                       >  5:206750/1‑149 (MQ=255)
    ggTTCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTc                                                                                                                                                    <  5:314006/148‑1 (MQ=255)
    ggTTCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCg                                                                                                                                                   <  6:206750/149‑1 (MQ=255)
      ttCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCt                                                                                                                                                 <  8:151574/149‑1 (MQ=255)
      gtCGCCCGCGCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGGCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTGCAGACCCCATCAGAGCGACCGCGAGATCGCt                                                                                                                                                 <  7:80937/148‑1 (MQ=255)
        cGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTc                                                                                                                                                <  3:30930/148‑1 (MQ=255)
        cGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTACACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGACCGCGAGATCGCTc                                                                                                                                                <  2:176823/148‑1 (MQ=255)
              cTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGc                                                                                                                                         <  2:184867/149‑1 (MQ=255)
               tCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGc                                                                                                                                         >  8:305371/1‑148 (MQ=255)
               tCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATATTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTAAACAGGc                                                                                                                                         >  1:351029/1‑148 (MQ=255)
                  cccTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATg                                                                                                                                     >  7:68880/1‑149 (MQ=255)
                         tAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATg                                                                                                                              >  4:157787/1‑149 (MQ=255)
                         tAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATg                                                                                                                              >  4:151780/1‑149 (MQ=255)
                         tAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATg                                                                                                                              <  3:228152/149‑1 (MQ=255)
                           aCAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATgg                                                                                                                             <  6:395749/148‑1 (MQ=255)
                           aCAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATgg                                                                                                                             >  2:310114/1‑148 (MQ=255)
                                  cATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCAGTTATAGATCTTTGAAGCCGCCGCACACGCATTATCAAGAGGTAGTTCAGCCCCCATCAGAGGGATCTCGCGGTCGCTCAACAGGGCATGCTGAATGGGAtgcggg                                                                                                                     >  6:66690/1‑144 (MQ=255)
                                   aTCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGc                                                                                                                    >  7:342427/1‑149 (MQ=255)
                                      aaGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCt                                                                                                                 >  4:267522/1‑149 (MQ=255)
                                      aaGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCACATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCt                                                                                                                 >  2:74826/1‑149 (MQ=255)
                                          aTTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑Tc                                                                                                            <  3:267522/149‑1 (MQ=255)
                                              cccAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCcg                                                                                                        >  7:241526/1‑149 (MQ=255)
                                               ccAGTCCTGCACGGCGCACAGAGGCATCCATTTAAAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCcgc                                                                                                       >  1:59096/1‑149 (MQ=255)
                                                cAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCAGGCTCAATGGGATTCACGCCCTTT‑TCCCcgcg                                                                                                      <  6:70827/149‑1 (MQ=255)
                                                     cTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCTCGCAACAGGCAATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGATCAg                                                                                                 >  7:114736/1‑149 (MQ=255)
                                                     cTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATgg                                                                                                                             <  5:397075/122‑1 (MQ=255)
                                                     cTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATgg                                                                                                                             >  6:397075/1‑122 (MQ=255)
                                                       gCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGc                                                                                                >  2:339729/1‑148 (MQ=255)
                                                       gCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TACCCGCGCTCAGc                                                                                                >  5:22576/1‑148 (MQ=255)
                                                         aCGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTc                                                                                                                                                    >  6:374921/1‑95 (MQ=255)
                                                         aCGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTc                                                                                                                                                    <  5:374921/95‑1 (MQ=255)
                                                            gcgcACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCACAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGTGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATAtt                                                                                          >  4:216989/1‑149 (MQ=255)
                                                               cacaGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTga                                                                                       <  3:151780/149‑1 (MQ=255)
                                                               cacaGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTga                                                                                       <  3:157787/149‑1 (MQ=255)
                                                                      gCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTc                                                                                >  6:171095/1‑149 (MQ=255)
                                                                       cATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTcc                                                                               <  5:66690/149‑1 (MQ=255)
                                                                          ccATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTc                                                                                <  3:166460/145‑1 (MQ=255)
                                                                          ccATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTc                                                                                >  4:166460/1‑145 (MQ=255)
                                                                            aTTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACAt                                                                          <  8:241526/149‑1 (MQ=255)
                                                                             tttATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACAt                                                                          >  2:295413/1‑148 (MQ=255)
                                                                                 taGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑cc                                                                     <  1:339729/148‑1 (MQ=255)
                                                                                  aGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGc                                                                   >  4:314529/1‑149 (MQ=255)
                                                                                   gATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGcc                                                                  <  3:216989/149‑1 (MQ=255)
                                                                                    aTCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCt                                                                 >  6:285903/1‑149 (MQ=255)
                                                                                    aTCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCt                                                                 >  6:9064/1‑149 (MQ=255)
                                                                                          gAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATg                                                            <  7:305371/148‑1 (MQ=255)
                                                                                              ccgccgCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCt                                                       >  2:338454/1‑149 (MQ=255)
                                                                                                 ccgcAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCtttt                                                    >  1:241496/1‑149 (MQ=255)
                                                                                                    cAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTa                                                 <  8:68880/149‑1 (MQ=255)
                                                                                                     aaaCGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTTCTCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTa                                                 <  1:310114/149‑1 (MQ=255)
                                                                                                     aaaCGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAg                                                <  2:241496/149‑1 (MQ=255)
                                                                                                         gCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCttt                                                     >  3:120398/1‑140 (MQ=255)
                                                                                                         gCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCttt                                                     <  4:120398/140‑1 (MQ=255)
                                                                                                         acATTATCAAGAGGTAGTTCAGACCCCATAAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGCGATTCAAGCCCTTA‑TCCCCGCGCTCAGCAATAATTGAGAATTTCCGACATCA‑CCGCCTTAATGCACCTTTTGTAGGCt                                             >  7:17785/2‑148 (MQ=255)
                                                                                                               tCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAAcc                                      >  6:336491/1‑149 (MQ=255)
                                                                                                               tCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATCACCCTGTTGTAGGCTTCaatca                                      >  8:217434/1‑146 (MQ=255)
                                                                                                               tCAAGAGGTAGTTCAGACCCCATCAGAGAGATCTCGATGTCGCTCAACAGGCCATGCTCAATGTGATTCACGCACTTT‑TCCCCGCGCTCAGCAATATTTGAGAAGTTCCGAAATCA‑CCTCGTTAATGACCATTTTGTAGGCTTCAAAcc                                      >  8:331983/1‑149 (MQ=255)
                                                                                                                 aaGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCTCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCtt                                    <  2:59096/149‑1 (MQ=255)
                                                                                                                 aaGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCtt                                    >  4:194839/1‑149 (MQ=255)
                                                                                                                 aaGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCtt                                    <  3:314529/149‑1 (MQ=255)
                                                                                                                 aaGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCt                                     <  7:230970/148‑1 (MQ=255)
                                                                                                                   gagGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAAGAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCATCAATATTTGAGAATTTACGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCtt                                    <  1:74826/147‑1 (MQ=255)
                                                                                                                      gTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCACTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGaa                                 >  6:14269/1‑147 (MQ=255)
                                                                                                                         gTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTc                            <  5:9064/149‑1 (MQ=255)
                                                                                                                         gTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTc                            <  5:171095/149‑1 (MQ=255)
                                                                                                                         gTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTc                            >  7:21307/1‑149 (MQ=255)
                                                                                                                         gTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTc                            <  7:217434/149‑1 (MQ=255)
                                                                                                                             aGACCCCCTCAGAGCGAGCTCGCGGTCGCTCAACCGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGaa                        <  8:114736/149‑1 (MQ=255)
                                                                                                                              gACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAAc                       <  2:351029/149‑1 (MQ=255)
                                                                                                                               aCCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACg                      >  7:310219/1‑149 (MQ=255)
                                                                                                                                 cccATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TGCCCGCTCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCATAATCCCCCTTTAGTAGCCTTCAAACCTTGAACAGTCGGAACGtt                    >  6:62879/1‑149 (MQ=255)
                                                                                                                                 cccATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCtt                                                      <  2:185582/115‑1 (MQ=255)
                                                                                                                                 cccATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCtt                                                      >  1:185582/1‑115 (MQ=255)
                                                                                                                                 cccATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCATT‑TCCCCGCGCTCAGCAAAATTTGAGAATTTCCGACATCA‑CCGCCTTATTGCCCAGTGTGTAGGGTTCAAACCTTGAACAATCTGAACggt                    >  4:46249/1‑147 (MQ=255)
                                                                                                                                   cATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGtttt                  >  6:280208/1‑149 (MQ=255)
                                                                                                                                    aTCAGAGCGATCTCGCGGTCGCTCAACAGGCCATTCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTc                 <  8:17785/149‑1 (MQ=255)
                                                                                                                                     tCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCACCCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTc                 <  1:295413/149‑1 (MQ=255)
                                                                                                                                     gttgagCGACCGCGAGATCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCg                >  3:143384/4‑149 (MQ=255)
                                                                                                                                     gttgagCGACCGCGAGATCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCg                >  3:389667/4‑149 (MQ=255)
                                                                                                                                       agagCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGcc              >  2:337864/1‑149 (MQ=255)
                                                                                                                                        gagCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCt             <  5:336491/149‑1 (MQ=255)
                                                                                                                                         agCGATCTCGCGGTCACTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTa            <  5:14269/149‑1 (MQ=255)
                                                                                                                                           cGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGcc              >  2:176010/1‑145 (MQ=255)
                                                                                                                                             aTCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATg        >  8:314604/1‑149 (MQ=255)
                                                                                                                                                tcGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGCCATCA‑CCGCATAAATGCCCCTTTTGTAGGCTTCAAACATTCAAAAGTCTGAACGTTTTCGCCTAGATGGAc     >  7:258990/1‑149 (MQ=255)
                                                                                                                                                  gcgGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGAc     <  5:285903/147‑1 (MQ=255)
                                                                                                                                                   cgGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGa  <  1:338454/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GTTAGGTTCGCCCGCTCGCCCTCGGTAACAATCGCATCAAGGATTACCCAGTCCTGCACGGCGCACAGAGGCATCCATTTATAGATCTTTGAAGCCGCCGCAAACGCATTATCAAGAGGTAGTTCAGACCCCATCAGAGCGATCTCGCGGTCGCTCAACAGGCCATGCTCAATGGGATTCACGCCCTTT‑TCCCCGCGCTCAGCAATATTTGAGAATTTCCGACATCA‑CCGCCTTAATGCCCCTTTTGTAGGCTTCAAACCTTGAACAGTCTGAACGTTTTCGCCTAGATGGACGGA  >  NC_002947/5074382‑5074677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑