Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,194,887 T→G 11.5% intergenic (+29/+24) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,194,8870TG11.5% 147.0 / 12.9 61intergenic (+29/+24)PP_4574/PP_4575hypothetical protein/urea amidolyase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base G (3/4);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-01

CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194739‑5195035
                                                                                                                                                    |                                                                                                                                                    
cccAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCt                                                                                                                                                      >  6:339732/1‑149 (MQ=255)
  cAAGCAGGCCTGGGAGTACACCGCCTCGACCCAACTGGTGCGCGACCTGATCGAGGACAAGTTGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCg                                                                                                                                                    >  4:387061/1‑149 (MQ=255)
           cTGGGAGTACATCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           <  1:393241/149‑1 (MQ=255)
           cTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGaaa                                                                                                                                           <  6:339585/149‑1 (MQ=255)
           cTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTGGCCTGTACTGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           <  7:64650/149‑1 (MQ=255)
           cTGGGAGAACACCGCCTCGACCCATCTGGTGCGCGACCTGAGCGATGACAAGATGTATCCGCTGACCCTGCACGGGGTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGGTGCCTGTACTGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           <  5:208638/149‑1 (MQ=255)
              ggAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACTATATGTATCCGCTGTCCCTGCATGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAg                                                                                                                                          <  1:126507/147‑1 (MQ=255)
               gcgTACCCCGCCCCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAgg                                                                                                                                         <  4:284932/145‑1 (MQ=255)
               gAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTGGCCCGCGAAAgg                                                                                                                                         >  3:284932/1‑147 (MQ=255)
                     acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcagcag                                                                                                                                 >  6:135793/1‑149 (MQ=255)
                     acCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAACGGCcagcag                                                                                                                                 >  3:208791/1‑149 (MQ=255)
                       cGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTc                                                                                                                               >  4:350679/1‑149 (MQ=255)
                       cGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGACCGCGAAAGGGCCAGCAGTc                                                                                                                               >  4:344980/1‑149 (MQ=255)
                         ccTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCt                                                                                                                              >  3:27888/1‑148 (MQ=255)
                           tCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGAAGTCTTAc                                                                                                                           >  5:196361/1‑149 (MQ=255)
                             taCCCATCTGGGGCGCGACCTGATCGAGCACAAGAGGTATCCGCTGCCACTGCATGGTTTGGATGAGGGGATGCGCTACCTTGCAATGTTAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCTAAAGGGCCAGCAGTCTTAc                                                                                                                           <  2:182103/146‑1 (MQ=255)
                              aCCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                        <  7:310892/149‑1 (MQ=255)
                              aCCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGAc                                                                                                                        <  5:339732/149‑1 (MQ=255)
                               cccATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAggg                                                                                                                                        <  7:6267/132‑1 (MQ=255)
                               cccATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAggg                                                                                                                                        >  8:6267/1‑132 (MQ=255)
                                       gTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGc                                                                                                                  <  5:135793/146‑1 (MQ=255)
                                       gTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCCGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGc                                                                                                                  <  3:307379/146‑1 (MQ=255)
                                          cgcgACCTGATCGAGGACAAGTTGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAg                                                                                                             <  3:387061/148‑1 (MQ=255)
                                           gcgACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAgg                                                                                                            >  5:341346/1‑148 (MQ=255)
                                                  gATCGAGGACGAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCTCGGGCTCGCCCGCGAAAGGGCCAGACGTCTTACGACCACCGCCCCAGTTAAAgct                                                                                                     >  7:169182/1‑148 (MQ=255)
                                                  gATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctg                                                                                                    >  3:243433/1‑149 (MQ=255)
                                                   aTCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           >  8:277276/1‑109 (MQ=255)
                                                   aTCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCGAGCCCGCGaaa                                                                                                                                           <  7:277276/109‑1 (MQ=255)
                                                    tCGAGGACAAGATGTATGCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACATGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgc                                                                                                   <  4:27888/148‑1 (MQ=255)
                                                    tCGAGCACACGAGGTTGCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAgctgct                                                                                                  <  4:208791/149‑1 (MQ=255)
                                                     cGAGGACAAGATGTATCGGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCcag                                                                                                                                    <  6:309253/114‑1 (MQ=255)
                                                         gACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCATCGCCCCAGGTAAAGCTGCTGctct                                                                                             <  7:241742/149‑1 (MQ=255)
                                                          aCAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCCACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCtctc                                                                                            >  1:144363/1‑149 (MQ=255)
                                                               aTGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAATGGCCAGCAGTCTTACGACCAACGCCCCAGGAAAAGCTGCTGCTCTCGCCAg                                                                                       >  6:212815/1‑149 (MQ=255)
                                                               aTGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                       <  8:156403/149‑1 (MQ=255)
                                                                tGTATCCGCTGACCCTGCAAGGGTTGGAGGACGTGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAg                                                                                       <  2:286682/148‑1 (MQ=255)
                                                                 gTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGc                                                                                     >  5:98784/1‑149 (MQ=255)
                                                                 gTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGc                                                                                     >  7:10069/1‑149 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTCCGACCAACGCCCCAGGTAAAGCTGCGGCTCTCGCCAGGCCTc                                                                                  <  3:350679/149‑1 (MQ=255)
                                                                    tCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTc                                                                                  <  3:344980/149‑1 (MQ=255)
                                                                       gCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTc                                                                               <  8:10069/149‑1 (MQ=255)
                                                                             ccTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACCCCCCACGAAAACCTGCTGCTATCGCCAGGCATCGTCCTGccc                                                                         >  2:250649/1‑147 (MQ=255)
                                                                                          gaggaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAAc                                                            >  6:101671/1‑149 (MQ=255)
                                                                                             gaCGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGc                                                         >  2:7960/1‑149 (MQ=255)
                                                                                                    tGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCg                                                  >  1:142750/1‑149 (MQ=255)
                                                                                                             tGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCa                                         >  7:48682/1‑149 (MQ=255)
                                                                                                               tCCAAGAGAGCTTGCCTCTACCGGCCCTTTCGCGGGCTCCCCCCCGAAAGGGCCAGCAGTCTAACGACCAACGACCCAGCTAAAGCTGATGCTCACGCCAGGCCTCGTCCAGCACCACCg                                                                    >  8:274904/1‑120 (MQ=255)
                                                                                                                   aGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGCAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACt                                   >  8:202310/1‑149 (MQ=255)
                                                                                                                    gTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGGCCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCCGGCCTCGTCCGGCACCCCCGCCCAGACCCGCACCACCCTCCCCGGCATGCCCTg                                  >  4:366515/1‑149 (MQ=255)
                                                                                                                    gTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGCCCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCCCCGTCCCCGGCATGCACTg                                  >  4:230711/1‑149 (MQ=255)
                                                                                                                        ggTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGcc                              <  6:98784/149‑1 (MQ=255)
                                                                                                                            gCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTgc                                 >  3:171629/1‑142 (MQ=255)
                                                                                                                            gCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTgc                                 <  4:171629/142‑1 (MQ=255)
                                                                                                                             ccTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGCAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTg                         <  7:202310/149‑1 (MQ=255)
                                                                                                                              gactgctggCCCTTTCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCCGGCCTCGTCCTGCACCACCGCCCTGCACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgc                        >  5:100220/8‑149 (MQ=38)
                                                                                                                              cTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgc                        <  6:378214/149‑1 (MQ=255)
                                                                                                                                 tcccGGCCCTTTGGGTGGCTCGCCCGGGATAGTGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgc                      <  8:169182/146‑1 (MQ=255)
                                                                                                                                   ccGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACCCCCCAGGTTAAGCTGCTGCTCTCGACAGGCCTCGTCCTGCCCCACCGCCCTGAACCGCACCCCCGTCCCCGGCATGCACTGCGCCCGTTgcgccac                   >  3:34335/1‑148 (MQ=255)
                                                                                                                                    cGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagc                  <  6:36948/149‑1 (MQ=255)
                                                                                                                                      gCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTgcgccagcgc                >  4:25844/1‑149 (MQ=255)
                                                                                                                                        ccTTTCGCGGGCGAGCCCGCGAAAGGGCCGGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGACCCGCCCCACCGTCCCCGGCATGCACTGCGCCCGTTgcgcaagcgc                >  8:373613/1‑147 (MQ=255)
                                                                                                                                            tCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGccagcgccagcg           <  5:101671/148‑1 (MQ=255)
                                                                                                                                                    tCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTTGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAgcg  >  8:298912/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAAGCAGGCCTGGGAGTACACCGCCTCGACCCATCTGGTGCGCGACCTGATCGAGGACAAGATGTATCCGCTGACCCTGCAAGGGTTGGAGGACGGGATGCGCTACCTGTCCAAGTGAGGTTGCCTGTACCGGCCCTTTCGCGGGCTCGCCCGCGAAAGGGCCAGCAGTCTTACGACCAACGCCCCAGGTAAAGCTGCTGCTCTCGCCAGGCCTCGTCCTGCACCACCGCCCTGAACCGCACCACCGTCCCCGGCATGCACTGCGCCAGTTGCGCCAGCGCCAGCGGCGTCAGCG  >  NC_002947/5194739‑5195035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑