Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,199,645 G→C 12.9% intergenic (+20/+53) PP_4579 → / ← PP_4580 LysR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,199,6450GC12.9% 140.9 / 10.8 53intergenic (+20/+53)PP_4579/PP_4580LysR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/26);  new base C (2/5);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

AGCCTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCA  >  NC_002947/5199500‑5199786
                                                                                                                                                 |                                                                                                                                             
aGCCTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCgg                                                                                                                                            <  3:281672/149‑1 (MQ=255)
    ttGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCt                                                                                                                                        >  5:358853/1‑149 (MQ=255)
            gtggtggCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCggg                                                                                                                                >  1:26447/1‑149 (MQ=255)
               gtggcgtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCAt                                                                                                                             <  6:296537/149‑1 (MQ=255)
               gtggcgtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGCCCTTTCGCGGGCAt                                                                                                                             <  1:95559/149‑1 (MQ=255)
                 ggcgtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCAt                                                                                                                             <  4:165787/147‑1 (MQ=255)
                 ggcgtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCAt                                                                                                                             >  3:165787/1‑147 (MQ=255)
                  gcgtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGCCCTTTCGCGGGCATGcc                                                                                                                          >  8:295293/1‑149 (MQ=255)
                    gtggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCcg                                                                                                                        <  4:190872/149‑1 (MQ=255)
                     tggcAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGCCCTTTCGCGGGCATGCCcgc                                                                                                                       <  8:331473/149‑1 (MQ=255)
                        cAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGa                                                                                                                     >  7:185199/1‑148 (MQ=255)
                         aGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGaaa                                                                                                                   <  8:185199/149‑1 (MQ=255)
                         aGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGCCCTTTCGCGGGCATGCCCGCGaaa                                                                                                                   <  7:295293/149‑1 (MQ=255)
                         aGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTACCGGCCCTTTCGCGGGCATGCCCGCGaaa                                                                                                                   <  8:325013/149‑1 (MQ=255)
                            ccGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAgg                                                                                                                 <  2:114653/148‑1 (MQ=255)
                            ccGGGGTGGCGTTGGTCGATGAGGGGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAgg                                                                                                                 <  8:266165/148‑1 (MQ=255)
                                     cGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCggtacag                                                                                                       <  5:234670/149‑1 (MQ=255)
                                       ttGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCACCTGTACCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCg                                                                                                             <  2:317006/141‑1 (MQ=255)
                                       ttGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCACCTGTACCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCg                                                                                                             >  1:317006/1‑141 (MQ=255)
                                                 aGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGCTCGGGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGaa                                                                                            <  7:251337/148‑1 (MQ=255)
                                                  ggtggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCATTAGCGGGCATTCCCGGGAAAGGGGCCGTTCAGGTTCAGCAGAAca                                                                                          >  3:197941/1‑148 (MQ=255)
                                                     ggtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAg                                                                                       >  3:81051/1‑149 (MQ=255)
                                                      gtTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGAGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGTGCATGCCCGCGAAAGGGCAGGTTCAGGTACAGCAGAACATAgc                                                                                      >  8:124500/1‑149 (MQ=255)
                                                      gtTGGGGTTTGCCGGCAGGTGTTGGCGCGCTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACAAACAGTCTCCGCCTGTAGCGGCCCATTAGCGGGGAAGCCCCAGCAAAGGCCCGTAGAAGTAAAACAGAACATAga                                                                                      >  4:15510/1‑148 (MQ=255)
                                                       ttGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAgcg                                                                                     >  4:316940/1‑149 (MQ=255)
                                                           ggTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAg                                                                                 >  3:296582/1‑149 (MQ=255)
                                                                 ccGGCAGGTGTTGGCGCGGTCTGCGCGGGATGTGGGGGGGCAGCGGAGCGGGCTGGGCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTTGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGctctc                                                                            <  5:305744/148‑1 (MQ=39)
                                                                  cGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCtt                                                                          <  1:77877/149‑1 (MQ=255)
                                                                   ggCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCttt                                                                         >  1:270730/1‑149 (MQ=255)
                                                                     cagggtttGGGGCGGTATGGGCGGGTGGGGGGTGGGCTTGGGATCGTGCTGGTCTGACCAACTGTCTCCGCGTGTAGCGGCCCTTTCGCGGGCATGCCCGCTAATGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTca                                                                       <  8:141330/143‑1 (MQ=255)
                                                                     cAGGTGTTGGCGCGGTAGGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTca                                                                       <  6:126558/149‑1 (MQ=255)
                                                                        gtgtTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTcacca                                                                    <  4:81051/149‑1 (MQ=255)
                                                                                   ggaTGCGCGGGATGTGGCTGGGCAGCGGATCGTCCTGGTCTGCCCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGcgccgc                                                         <  8:66335/147‑1 (MQ=255)
                                                                                        cgcgGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCcgcccgcc                                                    <  2:270730/149‑1 (MQ=255)
                                                                                          cgGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGGCCGTACAGGTACAGCAGAACATAGCGCCAGGAGGAGTTCACCACCCAGCGCcgcccccc                                                    >  6:19139/1‑147 (MQ=37)
                                                                                             gATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGa                                               <  2:26447/149‑1 (MQ=255)
                                                                                                   ggtgggCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCATTAGCGGGGAGGCCCCCGAAAGGGCCGGTAAAGGGACAGCAGAACGTAGCGCCAGCTGGGGTTGACCCCCCAGGGCCGCCCGCCCAgggtgagg                                          >  2:351882/1‑142 (MQ=255)
                                                                                                      gggCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                                 <  6:107592/138‑1 (MQ=255)
                                                                                                      gggCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAg                                                 >  5:107592/1‑138 (MQ=255)
                                                                                                                    gCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCaa                         >  7:27805/1‑148 (MQ=255)
                                                                                                                     cTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGTCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACa                       <  4:296582/149‑1 (MQ=255)
                                                                                                                             aCCAACAGTCTCCGCCTGTCGCGGCCCTTTCGCGGGCATGCACGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCTCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTc                <  6:358853/148‑1 (MQ=255)
                                                                                                                             aCCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCTAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTc                <  5:19139/148‑1 (MQ=255)
                                                                                                                             aCCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCGTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCGTTCg               >  7:157148/1‑149 (MQ=255)
                                                                                                                              ccAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATcagccag                                       <  5:28437/124‑1 (MQ=255)
                                                                                                                               cAACAGTCTCCGCATGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTACACCACCCTGCGCCGCCCGCCCAGGATCAGCCACACAATCCACAGCAACCCGCATTCGAc             >  5:146277/1‑149 (MQ=38)
                                                                                                                                agcacgatcCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGAcc            <  7:149393/142‑1 (MQ=255)
                                                                                                                                  cAGTCTCCGCCTGTAGCGGCCCTTTAGCGGGCATGCCCGCGCAAGGGCCGGTACAGGTACAGGAGAACTTAGCGCCCGCTCTCTTTGACCACCCTGCGCCGCCCGCCCAGGCGCAGCCGGCCCATCCCCAGCAACAGGCTTTCGACca           >  3:93934/1‑148 (MQ=255)
                                                                                                                                  cAGTCTCCGCCCGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCACTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACca           >  1:62954/1‑148 (MQ=255)
                                                                                                                                     tctcCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACaaa       <  3:316940/149‑1 (MQ=255)
                                                                                                                                       tcCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACCGGCTTTCGCGCACAAAgg     >  3:64617/1‑149 (MQ=255)
                                                                                                                                          gCCTGTAGCGGCCCTTTCGCTGGCATGCCAGCGACAGGGCCGGTACAGGTACAGCAGAACATAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCa  <  7:124500/149‑1 (MQ=255)
                                                                                                                                          gCCTGTAGCGGCCCTTTCGCGGGCAGGCCCGCTAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCa  <  5:16389/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AGCCTTGAGCTGGTGGTGGCGTGGCAGACCGGGGTGGCGTTGGTCGATGAGGTGGTTGGGGTTTGCCGGCAGGTGTTGGCGCGGTATGCGCGGGATGTGGGTGGGCAGCGGATCGTGCTGGTCTGACCAACAGTCTCCGCCTGTAGCGGCCCTTTCGCGGGCATGCCCGCGAAAGGGCCGGTACAGGTACAGCAGAACTTAGCGCCAGCTCTCTTTCACCACCCTGCGCCGCCCGCCCAGGATCAGCCAGCCAATCCCCAGCAACAGGCTTTCGACCACAAAGGCCA  >  NC_002947/5199500‑5199786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑