Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,297,195	G→C	6.7%	A56A (GCC→GCG) ‡	TpbB ←	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,297,195	0	G	C	6.7%	231.0 / 6.3	75	A56A (GCC→GCG) ‡	TpbB	diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base C (3/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01

TTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCG  >  NC_002947/5297048‑5297335                                                                                                                                                    |                                                                                                                                             ttGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCggg                                                                                                                                             <  5:387029/149‑1 (MQ=255)     cAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGAt                                                                                                                                         <  1:18309/149‑1 (MQ=255)        tGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCaa                                                                                                                                      >  4:173241/1‑149 (MQ=255)         gcgcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                     >  3:29180/1‑149 (MQ=255)          cgcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCGTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                     <  8:220489/148‑1 (MQ=255)          cgcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAAc                                                                                                                                     <  2:344520/148‑1 (MQ=255)          cgcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAACt                                                                                                                                    <  3:173241/149‑1 (MQ=255)           gcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTg                                                                                                                                   >  7:239199/1‑149 (MQ=255)               gTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAgg                                                                                                                               <  7:257474/149‑1 (MQ=255)                      gCCCTCGTTGTTGAAGACCTTGGCCTCGGCGACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATg                                                                                                                          <  6:275071/147‑1 (MQ=255)                      gCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATg                                                                                                                          <  3:77472/147‑1 (MQ=255)                      gCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATg                                                                                                                          <  2:350727/147‑1 (MQ=255)                       cccTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGtt                                                                                                                       <  8:36861/149‑1 (MQ=255)                            gttgttGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCa                                                                                                                   >  4:120872/1‑148 (MQ=255)                             ttgttgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATa                                                                                                                 <  7:23416/149‑1 (MQ=255)                               gttgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAg                                                                                                                <  1:231942/148‑1 (MQ=255)                                   aaGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGacac                                                                                                            <  1:214632/148‑1 (MQ=255)                                        cTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAggg                                                                                                      >  1:2255/1‑149 (MQ=255)                                           ggCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGc                                                                                                     <  3:120872/147‑1 (MQ=255)                                            gCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAg                                                                                                  <  3:307610/149‑1 (MQ=255)                                             ccTCGTCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGc                                                                                                 <  8:239199/149‑1 (MQ=255)                                                       ttcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCcag                                                                                         >  5:265168/1‑147 (MQ=255)                                                        tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCcagcag                                                                                      >  6:325162/1‑149 (MQ=255)                                                         cttcGGTACTGGCAATGAACGCCAGCGATTCGTTGGCGGCGGAGCTGTCATCGAACACCAGCGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGTGCTCGCCctgcgt                                                                                      <  7:333061/148‑3 (MQ=255)                                                            cGGTACTGGCAATCAACGCCAGCGATTCGTAGGCGGCTTCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAgg                                                                                     <  7:352541/146‑1 (MQ=255)                                                              gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTGATCGAACACCACAGCCGCTTCTACGGTGTAGTTGTTCGAGCGGGCGAGCAACTGCAGGGTATGGTTGGCATAGACACGCAGGGCGAGCACGGCCAGGAGGGTGAg                                                                                >  2:277406/1‑149 (MQ=255)                                                               tACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                               <  4:312337/149‑1 (MQ=255)                                                                 cTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGga                                                                             <  5:353088/149‑1 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                               >  2:122524/1‑146 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGAAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                               >  3:200742/1‑146 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCAGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                               >  1:218534/1‑146 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGcc                                                                                            >  1:17190/1‑131 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGcc                                                                                            <  8:347333/131‑1 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGcc                                                                                            >  7:347333/1‑131 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGcc                                                                                            <  2:17190/131‑1 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                           >  3:340697/1‑148 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                           >  5:237485/1‑148 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgag                                                                            <  4:237607/147‑1 (MQ=255)                                                                      aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAc                                                                        <  1:104588/149‑1 (MQ=255)                                                                             gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                 <  4:93296/149‑1 (MQ=255)                                                                              ccAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAgg                                                                >  5:108889/1‑149 (MQ=255)                                                                               cAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGc                                                               >  6:114968/1‑149 (MQ=255)                                                                               cAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGc                                                               >  7:245784/1‑149 (MQ=255)                                                                                     ttCGTTTGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACgg                                                         <  4:271617/149‑1 (MQ=255)                                                                                            gcggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaagcaa                                                  <  4:200742/149‑1 (MQ=255)                                                                                              ggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                <  6:127422/149‑1 (MQ=255)                                                                                              ggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                <  5:342009/149‑1 (MQ=255)                                                                                               gcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGc                                               >  5:257839/1‑149 (MQ=255)                                                                                                          aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                <  4:28264/137‑1 (MQ=255)                                                                                                          aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                >  3:28264/1‑137 (MQ=255)                                                                                                          aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                    >  2:178903/1‑149 (MQ=255)                                                                                                           tCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTg                                   <  7:97071/149‑1 (MQ=255)                                                                                                             gAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGTTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTgg                                  <  7:231277/148‑1 (MQ=255)                                                                                                              aaCACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGc                                 >  2:14594/1‑148 (MQ=255)                                                                                                                  ccccaGCCGCTGCTACGGTGTAGTTGTTCGCGCGGGCGATCAACTGCAGGGTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAGCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACgg                            <  4:288767/146‑1 (MQ=255)                                                                                                                  ccacAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACgg                            <  1:14594/149‑1 (MQ=255)                                                                                                                   cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGCCGCTCAGGTGGCCACGGc                           >  5:307062/1‑149 (MQ=255)                                                                                                                    acaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGcc                          <  5:114968/149‑1 (MQ=255)                                                                                                                        ccGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGc                      <  6:237485/149‑1 (MQ=255)                                                                                                                        ccGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCAACGGCCCAg                       >  8:264448/1‑148 (MQ=255)                                                                                                                          gCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCAc                    >  2:274172/1‑149 (MQ=255)                                                                                                                              cTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGGGGCCACGGCCCAGCACCGa                 <  6:108889/148‑1 (MQ=255)                                                                                                                                 cGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgcg              >  1:169514/1‑148 (MQ=255)                                                                                                                                  ggTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa            <  5:325162/149‑1 (MQ=255)                                                                                                                                   gtgtAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGAACCCAGCGCAg           >  4:220175/1‑149 (MQ=255)                                                                                                                                    tgtAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg          >  6:306064/1‑149 (MQ=255)                                                                                                                                    tgtAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg          >  1:97204/1‑149 (MQ=255)                                                                                                                                      tAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAggg         <  2:169514/148‑1 (MQ=255)                                                                                                                                       aGTTGATCGCCCGCTCGATCAACTGCAGGTTAAGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTc       >  1:41231/1‑149 (MQ=255)                                                                                                                                        gTTGATCGCCCGCTCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCg      >  1:375384/1‑149 (MQ=255)                                                                                                                                        gTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTc       <  6:257839/148‑1 (MQ=255)                                                                                                                                           gATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAg                >  4:41354/1‑136 (MQ=255)                                                                                                                                           gATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAg                <  3:41354/136‑1 (MQ=255)                                                                                                                                           gATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc   >  2:60545/1‑149 (MQ=255)                                                                                                                                            aTCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc   <  2:97204/148‑1 (MQ=255)                                                                                                                                             tCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCCGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgcg  >  8:228869/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                             TTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTTGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCG  >  NC_002947/5297048‑5297335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑