Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,363,587 G→C 6.1% A60G (GCA→GGA)  glmM ← phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,363,5870GC 6.1% 211.5 / 4.1 66A60G (GCA→GGA) glmMphosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base C (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GCCGTTGTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACG  >  NC_002947/5363439‑5363724
                                                                                                                                                    |                                                                                                                                         
gCCGTTGTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTg                                                                                                                                           >  4:183725/1‑149 (MQ=255)
 ccGTTGTCATCGTGCGGGTTGTGGGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGc                                                                                                                                          <  4:77999/149‑1 (MQ=255)
 ccGTTGTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGGCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGc                                                                                                                                          <  8:97921/149‑1 (MQ=255)
 ccGTTGTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGc                                                                                                                                          <  2:229142/149‑1 (MQ=255)
      gTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGAc                                                                                                                                      <  3:170755/148‑1 (MQ=255)
         aTCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCg                                                                                                                                   >  6:65794/1‑148 (MQ=255)
          tCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGaa                                                                                                                                 <  1:247505/149‑1 (MQ=255)
            gTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACa                                                                                                                               >  4:279926/1‑149 (MQ=255)
             tGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACAt                                                                                                                              >  7:89787/1‑149 (MQ=255)
             tGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACAt                                                                                                                              <  8:214210/149‑1 (MQ=255)
               cGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATg                                                                                                                             >  4:19046/1‑148 (MQ=255)
                  gTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGc                                                                                                                         <  3:194531/149‑1 (MQ=255)
                        cGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCg                                                                                                                       >  1:36451/1‑145 (MQ=255)
                             cACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATAcgc                                                                                                              <  7:134800/149‑1 (MQ=255)
                              aCTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATAcgcg                                                                                                             >  3:166995/1‑149 (MQ=255)
                               cTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCgt                                                                                                            <  6:71133/149‑1 (MQ=255)
                                       gATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGc                                                                                                    <  3:183725/149‑1 (MQ=255)
                                        aTGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGcc                                                                                                   <  3:279926/149‑1 (MQ=255)
                                            cGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCccac                                                                                                >  3:122161/1‑148 (MQ=255)
                                            cGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCccac                                                                                                >  1:364037/1‑148 (MQ=255)
                                                ttCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGAATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGAGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCa                                                                                           >  5:131526/1‑149 (MQ=255)
                                                  cGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCAcg                                                                                         >  4:163175/1‑149 (MQ=255)
                                                      gTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCgg                                                                                      >  1:155687/1‑148 (MQ=255)
                                                       tGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGc                                                                                     >  7:303513/1‑148 (MQ=255)
                                                             gTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGcc                                                                              <  6:159095/149‑1 (MQ=255)
                                                                      aGGTAAGCGATGGCCGGTGTCGGCATCGGCACGAGCAGGATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCg                                                                     >  7:339894/1‑149 (MQ=255)
                                                                          aaGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAg                                                                 >  4:96241/1‑149 (MQ=255)
                                                                           aGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAgg                                                                <  4:224814/149‑1 (MQ=255)
                                                                             cGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGc                                                               >  2:111088/1‑148 (MQ=255)
                                                                               aTGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCAt                                                            >  3:273586/1‑149 (MQ=255)
                                                                               aTGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCAt                                                            >  8:122464/1‑149 (MQ=255)
                                                                                   ccGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccg                                                        <  7:122464/149‑1 (MQ=255)
                                                                                   ccGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccg                                                        >  2:302339/1‑149 (MQ=255)
                                                                                       tgtCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccggccg                                                    <  4:122161/149‑1 (MQ=255)
                                                                                       tgtCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAAAGGCCTTGCTTGCGGAAGGCCATgccggccg                                                    <  3:19046/149‑1 (MQ=255)
                                                                                         tCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGgccgc                                                   <  4:273586/148‑1 (MQ=255)
                                                                                           ggCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGgccgcc                                                  >  6:143409/1‑147 (MQ=255)
                                                                                           ggCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGccc                                                 <  2:364037/148‑1 (MQ=255)
                                                                                                  gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGCTACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                         >  4:146745/1‑149 (MQ=255)
                                                                                                  gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                         >  6:159558/1‑149 (MQ=255)
                                                                                                  gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                         <  2:248518/149‑1 (MQ=255)
                                                                                                   cccGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCt                                        >  8:147310/1‑149 (MQ=255)
                                                                                                         cagcaTCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCa                                  >  2:382332/1‑149 (MQ=255)
                                                                                                            caTCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCCCCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATg                                >  5:289456/1‑148 (MQ=255)
                                                                                                                  aTCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                         <  4:166995/149‑1 (MQ=255)
                                                                                                                  aTCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                         <  8:89787/149‑1 (MQ=255)
                                                                                                                  aTCGGCACCTGCCGAGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                         >  2:358528/1‑149 (MQ=255)
                                                                                                                     ggCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGc                       <  1:382332/148‑1 (MQ=255)
                                                                                                                      gCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATg                                                           >  6:178537/1‑111 (MQ=255)
                                                                                                                      gCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATg                                                           <  5:178537/111‑1 (MQ=255)
                                                                                                                          cTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATc                 >  4:276578/1‑149 (MQ=255)
                                                                                                                             ccGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCgg               <  2:141709/148‑1 (MQ=255)
                                                                                                                             ccGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCgg               <  1:111088/148‑1 (MQ=255)
                                                                                                                              cgcgGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGt             <  2:36451/149‑1 (MQ=255)
                                                                                                                                    cAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGc       <  3:163175/149‑1 (MQ=255)
                                                                                                                                    cAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGc       <  7:147310/149‑1 (MQ=255)
                                                                                                                                     aGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc      <  5:240144/149‑1 (MQ=255)
                                                                                                                                        ccGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGAc   >  4:265206/1‑149 (MQ=255)
                                                                                                                                         cGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTAtt          <  4:98234/141‑1 (MQ=255)
                                                                                                                                         cGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTAtt          >  3:98234/1‑141 (MQ=255)
                                                                                                                                         cGGCTTCGAGTGCGGACTAGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGAcg  >  1:120430/1‑149 (MQ=255)
                                                                                                                                          atgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTa            <  6:24989/135‑1 (MQ=255)
                                                                                                                                          atgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTa            >  5:24989/4‑138 (MQ=255)
                                                                                                                                           tgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGc                                   >  7:65365/3‑114 (MQ=255)
                                                                                                                                           tgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGc                                   <  8:65365/112‑1 (MQ=255)
                                                                                                                                                  gTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc      >  7:262592/1‑136 (MQ=255)
                                                                                                                                                  gTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc      <  8:262592/136‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
GCCGTTGTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACG  >  NC_002947/5363439‑5363724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑