Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,363,590 G→C 6.2% S59C (TCC→TGC)  glmM ← phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,363,5900GC 6.2% 206.9 / 4.4 65S59C (TCC→TGC) glmMphosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

GTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACGCGGCCACGAATGCC  >  NC_002947/5363445‑5363738
                                                                                                                                                 |                                                                                                                                                    
gTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGAc                                                                                                                                                    <  3:170755/148‑1 (MQ=255)
   aTCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCg                                                                                                                                                 >  6:65794/1‑148 (MQ=255)
    tCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGaa                                                                                                                                               <  1:247505/149‑1 (MQ=255)
      gTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACa                                                                                                                                             >  4:279926/1‑149 (MQ=255)
       tGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACAt                                                                                                                                            >  7:89787/1‑149 (MQ=255)
       tGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACAt                                                                                                                                            <  8:214210/149‑1 (MQ=255)
         cGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATg                                                                                                                                           >  4:19046/1‑148 (MQ=255)
            gTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGc                                                                                                                                       <  3:194531/149‑1 (MQ=255)
                  cGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCg                                                                                                                                     >  1:36451/1‑145 (MQ=255)
                       cACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATAcgc                                                                                                                            <  7:134800/149‑1 (MQ=255)
                        aCTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATAcgcg                                                                                                                           >  3:166995/1‑149 (MQ=255)
                         cTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCgt                                                                                                                          <  6:71133/149‑1 (MQ=255)
                                 gATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGc                                                                                                                  <  3:183725/149‑1 (MQ=255)
                                  aTGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGcc                                                                                                                 <  3:279926/149‑1 (MQ=255)
                                      cGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCccac                                                                                                              >  3:122161/1‑148 (MQ=255)
                                      cGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCccac                                                                                                              >  1:364037/1‑148 (MQ=255)
                                          ttCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGAATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGAGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCa                                                                                                         >  5:131526/1‑149 (MQ=255)
                                            cGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCAcg                                                                                                       >  4:163175/1‑149 (MQ=255)
                                                gTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCgg                                                                                                    >  1:155687/1‑148 (MQ=255)
                                                 tGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGc                                                                                                   >  7:303513/1‑148 (MQ=255)
                                                       gTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGcc                                                                                            <  6:159095/149‑1 (MQ=255)
                                                                aGGTAAGCGATGGCCGGTGTCGGCATCGGCACGAGCAGGATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCg                                                                                   >  7:339894/1‑149 (MQ=255)
                                                                    aaGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAg                                                                               >  4:96241/1‑149 (MQ=255)
                                                                     aGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAgg                                                                              <  4:224814/149‑1 (MQ=255)
                                                                       cGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGc                                                                             >  2:111088/1‑148 (MQ=255)
                                                                         aTGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCAt                                                                          >  8:122464/1‑149 (MQ=255)
                                                                         aTGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCAt                                                                          >  3:273586/1‑149 (MQ=255)
                                                                             ccGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccg                                                                      >  2:302339/1‑149 (MQ=255)
                                                                             ccGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccg                                                                      <  7:122464/149‑1 (MQ=255)
                                                                                 tgtCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATgccggccg                                                                  <  4:122161/149‑1 (MQ=255)
                                                                                 tgtCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAAAGGCCTTGCTTGCGGAAGGCCATgccggccg                                                                  <  3:19046/149‑1 (MQ=255)
                                                                                   tCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGgccgc                                                                 <  4:273586/148‑1 (MQ=255)
                                                                                     ggCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGgccgcc                                                                >  6:143409/1‑147 (MQ=255)
                                                                                     ggCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGccc                                                               <  2:364037/148‑1 (MQ=255)
                                                                                            gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGCTACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                                       >  4:146745/1‑149 (MQ=255)
                                                                                            gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                                       <  2:248518/149‑1 (MQ=255)
                                                                                            gCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGc                                                       >  6:159558/1‑149 (MQ=255)
                                                                                             cccGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCt                                                      >  8:147310/1‑149 (MQ=255)
                                                                                                   cagcaTCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCa                                                >  2:382332/1‑149 (MQ=255)
                                                                                                      caTCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCCCCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATg                                              >  5:289456/1‑148 (MQ=255)
                                                                                                            aTCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                                       <  8:89787/149‑1 (MQ=255)
                                                                                                            aTCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                                       <  4:166995/149‑1 (MQ=255)
                                                                                                            aTCGGCACCTGCCGAGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAg                                       >  2:358528/1‑149 (MQ=255)
                                                                                                               ggCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGc                                     <  1:382332/148‑1 (MQ=255)
                                                                                                                gCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATg                                                                         <  5:178537/111‑1 (MQ=255)
                                                                                                                gCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATg                                                                         >  6:178537/1‑111 (MQ=255)
                                                                                                                    cTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATc                               >  4:276578/1‑149 (MQ=255)
                                                                                                                       ccGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCgg                             <  2:141709/148‑1 (MQ=255)
                                                                                                                       ccGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCgg                             <  1:111088/148‑1 (MQ=255)
                                                                                                                        cgcgGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGt                           <  2:36451/149‑1 (MQ=255)
                                                                                                                              cAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGc                     <  3:163175/149‑1 (MQ=255)
                                                                                                                              cAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGc                     <  7:147310/149‑1 (MQ=255)
                                                                                                                               aGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc                    <  5:240144/149‑1 (MQ=255)
                                                                                                                                  ccGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGAc                 >  4:265206/1‑149 (MQ=255)
                                                                                                                                   cGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTAtt                        <  4:98234/141‑1 (MQ=255)
                                                                                                                                   cGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTAtt                        >  3:98234/1‑141 (MQ=255)
                                                                                                                                   cGGCTTCGAGTGCGGACTAGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGAcg                >  1:120430/1‑149 (MQ=255)
                                                                                                                                    atgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTa                          >  5:24989/4‑138 (MQ=255)
                                                                                                                                    atgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTa                          <  6:24989/135‑1 (MQ=255)
                                                                                                                                     tgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGc                                                 >  7:65365/3‑114 (MQ=255)
                                                                                                                                     tgttCGAGTCCGCACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGc                                                 <  8:65365/112‑1 (MQ=255)
                                                                                                                                            gTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc                    >  7:262592/1‑136 (MQ=255)
                                                                                                                                            gTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGcc                    <  8:262592/136‑1 (MQ=255)
                                                                                                                                               cGGTCTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACGCGGCCACGAAt     <  6:20158/148‑1 (MQ=255)
                                                                                                                                               cGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACGCGGCCACGAAt     <  6:131526/148‑1 (MQ=255)
                                                                                                                                                 gACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACGCGGCCACGAATGcc  >  8:292025/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTCATCGTGCGGGTTGTGCGAGGCACTGATGACGATGCCGGCTTCGGCGTGGAAGGTACGCGTGAGGTAAGCGATGGCCGGTGTCGGCATCGGCCCGAGCAGCATCACATCGGCACCTGCCGCGGACAGGCCGGCTTCGAGTGCGGACTCGAACATGTAGCCGGAGATACGCGTGTCCTTGCCCACCAGCACGCGGCAATGGCCTTGCTTGCGGAAGGCCATGCCGGCCGCCCAGCCAAGCTTCAGCATGAAGTCAGGCGTGATCGGGTATTCGCCGACGCGGCCACGAATGCC  >  NC_002947/5363445‑5363738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑