| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 358,091 | G→A | 12.5% | intergenic (+59/‑71) | tdcG‑I → / → PP_5441 | L‑serine dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 358,091 | 0 | G | A | 12.5% | 75.2 / 3.0 | 33 | intergenic (+59/‑71) | tdcG‑I/PP_5441 | L‑serine dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/17); new base A (2/2); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01 | |||||||||||
CGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGT > NC_002947/357943‑358238 | cGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg < 1:98769/149‑1 (MQ=255) gCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACtgtg > 4:330755/1‑148 (MQ=255) ccATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAAt < 4:388281/149‑1 (MQ=255) aTGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATcc < 2:266330/149‑1 (MQ=255) cgcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCt < 3:330755/148‑1 (MQ=255) gcgATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGt < 5:284623/149‑1 (MQ=255) cgATACCGGCGCCTTTATGCACGACATGTACTAAGAGAGATCGCGAGGCGGGCTGGCTGTCAGCGCTATAGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTa < 6:377172/149‑1 (MQ=255) tACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGa < 5:345476/149‑1 (MQ=37) ggCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTGTCTCCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGc < 1:117227/149‑1 (MQ=25) cgcCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCgggt < 4:4644/149‑2 (MQ=255) cgcCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGc < 4:376321/149‑1 (MQ=255) aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGccc < 7:211655/144‑1 (MQ=255) aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGaa < 7:308389/149‑1 (MQ=255) aCGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCCCTCCGACAGGCACTGTTCAATCCCTGGAGGATCGGGCTTGCCCgagat > 8:211655/1‑148 (MQ=38) ctactaGTACAATTAGACCTGGGGCGGGGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTTTGTGATG‑‑ACCTTGTGTGCCTGTTCGCGGGCAATCCCGCTCCTACAGTAAGTTTGAAATCCCTGTAGTTGCGGGTTTGCCCGCGAag < 6:217872/144‑1 (MQ=255) cTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTTCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATg > 8:19429/1‑149 (MQ=255) gcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAg > 2:250469/1‑108 (MQ=255) gcgcgGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAg < 1:250469/108‑1 (MQ=255) cggcggCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTg < 7:19429/149‑1 (MQ=255) gCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGTAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATg < 5:140597/132‑1 (MQ=255) gCCTCTTCGCGGGCAAGCCCCCAACTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCGATGCCACTTTATTGTGCGGTGATg > 6:140597/1‑132 (MQ=37) tctTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCCATCCCAGTTGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGAAGCCGGTGCCACACAACCGCCGATGCCTCGTTATGGTGCGGTGAATGGCCACCCGCCTAACGAtcg > 6:163709/1‑149 (MQ=25) ctCGCGGGCACGCCCGCTCATACAGGAACTGTGCAATCCCTTTAGGCGCGGGGTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgt < 4:279868/147‑1 (MQ=255) gcgGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGAtcgg > 3:279868/1‑144 (MQ=255) gggCAAGCCCGCTCCTACAGGATCAGTGCAATCCCTGTAGGAGCGGGCTTGACCGCGAACAGGCCCTTGAGGATGATGAAGGAGAAACACAACCGCCAATGCCTCTTTATGGTGCGGTGATGGGCGCCCCCCATACCGAAAGTCGGAt > 7:59014/1‑148 (MQ=37) ccGCTCCTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGATGATGCAGGTGCAACACAACCGCCTATGAAAATTTATGGTGCGGTGATGGGCGCCCCGCAAACCGAACGACGGATGACGCCAt > 8:244558/1‑148 (MQ=18) gCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGACCTTGAGTCTGATGCAGGTGCAACACAACCGACTATGCATCTTTATGGTGCGGTGATGGGAGCCCCTCCTACCGATCGTCGGATTTCGCGATAAg > 4:209851/1‑149 (MQ=38) cgccTCCAGCACCTGTGCAATCCCTGTCGGCGGGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgt < 1:150384/147‑1 (MQ=255) tCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAgtg > 4:30000/1‑149 (MQ=255) cTACAGGGATTGCACAGTTCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGtgtg > 8:319172/1‑149 (MQ=37) cTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGCTCGTCGGATGTCGCGATCAGtgtg > 8:359053/1‑149 (MQ=255) tACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTgg > 4:173793/1‑149 (MQ=255) cAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGc < 3:173793/149‑1 (MQ=255) gAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt < 2:168873/128‑1 (MQ=255) gAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGAt > 1:168873/1‑128 (MQ=255) tgtgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGGACAGTGTGGGCGTTGTCg > 1:135799/1‑149 (MQ=255) tgCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGt > 6:315130/1‑149 (MQ=255) | CGCACCATGCGCGATACCGGCGCCGATATGCACGACAAGTACAAAGAGACCTCGCGCGGCGGCCTGGCGGTCAGCGCTATCGAGTGCTGATTTTCTGAGCTG‑‑ACCTTCTGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAGGAACTGTGCAATCCCTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCTTGAGGCTGATGCAGGTGCAACACAACCGCCTATGCCTCTTTATGGTGCGGTGATGGGCGCCCCGCCTACCGATCGTCGGATGTCGCGATCAGTGTGGGCGTTGTCGGT > NC_002947/357943‑358238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |