Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,407,052 A→C 6.0% L122R (CTG→CGG)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,407,0520AC 6.0% 211.3 / 4.4 67L122R (CTG→CGG) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base C (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

ACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTC  >  NC_002947/5406904‑5407197
                                                                                                                                                    |                                                                                                                                                 
aCGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                                   <  6:108517/149‑1 (MQ=255)
 cGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCTGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                                   <  5:294510/148‑1 (MQ=255)
           ggCCACCAGAGCAGCGCGCCGGATAATGCTGGTCCTTGTCAGCCAGCCAAACGCGTTCAAGCATTTGCAAGGAGCGGGCGTCGGCTTATTTTGCGATGCGGCCCAAGGTGCGGATTTGCGCCAGGATGACGTTGCGAAGCACCGTCAgg                                                                                                                                        >  7:247523/1‑149 (MQ=255)
           ggCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCCTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgc                                                                                                                                        <  3:370510/149‑2 (MQ=255)
            gCCACCAGAGCATCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTAGCAAGGACCCGGCGTCGGCTAATTTGGCGCTGCTGCCCAAGGAGCGGATTAGCGCCAGGCTGACGTTGATCAGCACCGTAAGGt                                                                                                                                       >  6:345353/1‑149 (MQ=255)
            gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                         <  4:58663/147‑1 (MQ=255)
            gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                         <  7:17979/147‑1 (MQ=255)
            gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGt                                                                                                                                       <  1:63799/149‑1 (MQ=255)
            gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGt                                                                                                                                       >  7:101575/1‑149 (MQ=255)
            gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                         <  1:299002/147‑1 (MQ=255)
              caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGTTGACGTTGCGCAGCACCGTCAGGTGc                                                                                                                                     >  8:188498/1‑149 (MQ=255)
              caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGc                                                                                                                                     >  3:134531/1‑149 (MQ=255)
               accaGCGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGAGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCg                                                                                                                                    >  4:142268/1‑149 (MQ=255)
                 caGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                   >  3:65394/1‑148 (MQ=255)
                      cagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                             >  5:251273/1‑149 (MQ=255)
                      cagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                             >  4:87976/1‑149 (MQ=255)
                       agcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAgg                                                                                                                            <  3:331977/149‑1 (MQ=255)
                       agcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTATGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAgg                                                                                                                            >  1:124329/1‑149 (MQ=255)
                          gcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTg                                                                                                                         <  5:122814/149‑1 (MQ=255)
                            gcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGaa                                                                                                                       <  3:142268/149‑1 (MQ=255)
                                 aTAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACt                                                                                                                  <  4:272463/149‑1 (MQ=255)
                                   aaTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                <  2:33867/149‑1 (MQ=255)
                                   aaTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                <  2:27820/149‑1 (MQ=255)
                                    aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGa                                                                                                               <  3:87976/149‑1 (MQ=255)
                                       cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAaca                                                                                                            <  2:331903/149‑1 (MQ=255)
                                        tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                           >  8:208795/1‑149 (MQ=255)
                                            ccatgacagccagcCCAACGCCTTCGAGCAGTTGCAAGGAGCCGGCGTCGGCTTCTTTGGATCAGCGGCCCAAGGTGAGGATTTCCGCCAGGAAGACGTTGCGCAGCACCGTCAGGTGCGGGAAAAGGTTGAAAGACTCGAACACCATa                                                                                                       >  6:386045/7‑149 (MQ=255)
                                             cTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATAc                                                                                                      <  7:206023/149‑1 (MQ=255)
                                                gTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATAcc                                                                                                     <  4:65394/147‑1 (MQ=255)
                                                   agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                                >  4:78260/1‑149 (MQ=255)
                                                           cAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                   <  6:189375/106‑1 (MQ=255)
                                                           cAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                   >  5:189375/1‑106 (MQ=255)
                                                             aCGCGTTCGAGCAGTTGCAAGGAGCAGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCaa                                                                                      <  4:292985/149‑1 (MQ=255)
                                                              cgcgTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAAc                                                                                     >  3:313973/1‑149 (MQ=255)
                                                                 gTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTc                                                                                  <  3:78260/149‑1 (MQ=255)
                                                                  ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                                   >  5:168615/1‑147 (MQ=255)
                                                                  ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTca                                                                                 <  6:251273/149‑1 (MQ=255)
                                                                     gAGCAGTTGCAAGGAGCGGGCGTCGGGTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAAGACCATACCAATGCGCCGGCGCAACTTcagca                                                                              <  7:66145/149‑1 (MQ=255)
                                                                        cAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGtt                                                                           <  7:237062/149‑1 (MQ=255)
                                                                                     cGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCaa                                                              >  2:233738/1‑149 (MQ=255)
                                                                                      gggCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCAGACCAATGCGCCGGCGCACCTTCAGCAGTTCTGTACGGGGCAAc                                                             >  8:271032/1‑149 (MQ=255)
                                                                                        gCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGc                                                           >  4:107717/1‑149 (MQ=255)
                                                                                           tCGGCTTCTTTGGCGCTGGGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACAGCATACGAATGCGCCGTCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCtt                                                        <  6:126056/149‑1 (MQ=255)
                                                                                           tCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCtt                                                        >  1:106101/1‑149 (MQ=255)
                                                                                             ggCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTg                                                       <  4:134531/148‑1 (MQ=255)
                                                                                              gCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                      >  1:168398/1‑148 (MQ=255)
                                                                                              gCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAAGTTGAACTACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGATCTGAAAGGGGCACCGCCttcc                                                      >  6:18127/1‑146 (MQ=255)
                                                                                               cttcttTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCa                                                    <  7:208795/149‑1 (MQ=255)
                                                                                                ttcttTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                   >  3:270996/1‑149 (MQ=255)
                                                                                                ttcttTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                   >  3:265838/1‑149 (MQ=255)
                                                                                                   tttGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCtt                                                >  4:365806/1‑149 (MQ=255)
                                                                                                       gcgcTGCGGCCCAAGGTGCTGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                            <  6:133520/149‑1 (MQ=255)
                                                                                                       gcgcTGCGGCCCAAGGTGCGGATTTGCGCCAGGTTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGTCTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                            <  7:188498/149‑1 (MQ=255)
                                                                                                       gcgcTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                            <  8:101575/149‑1 (MQ=255)
                                                                                                       gcgcTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                            >  2:248924/1‑149 (MQ=255)
                                                                                                       ctgacaaggCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                            <  2:28183/142‑1 (MQ=255)
                                                                                                           tGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGt                                                                       <  4:200181/118‑1 (MQ=255)
                                                                                                           tGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGt                                                                       >  3:200181/1‑118 (MQ=255)
                                                                                                             cGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGc                                        <  3:107717/147‑1 (MQ=255)
                                                                                                              ggCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACg                                     >  3:202271/1‑149 (MQ=255)
                                                                                                                      gTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGccatccat                             >  5:236590/1‑149 (MQ=255)
                                                                                                                       tGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATg                            <  1:233738/149‑1 (MQ=255)
                                                                                                                             tttGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGAcc                      >  7:23513/1‑149 (MQ=255)
                                                                                                                                  gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc                 <  2:124329/149‑1 (MQ=255)
                                                                                                                                     aGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCtt              <  4:270996/149‑1 (MQ=255)
                                                                                                                                     aGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCtt              <  4:265838/149‑1 (MQ=255)
                                                                                                                                        cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt           >  5:350395/1‑149 (MQ=255)
                                                                                                                                        cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt           >  4:159471/1‑149 (MQ=255)
                                                                                                                                           aCGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTc          <  6:236590/147‑1 (MQ=255)
                                                                                                                                                  gcagcaCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTc  >  6:265376/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTC  >  NC_002947/5406904‑5407197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑