Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,420,452 G→C 6.1% A216G (GCC→GGC)  PP_4760 ← alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,420,4520GC 6.1% 214.2 / 4.0 66A216G (GCC→GGC) PP_4760alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base C (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

ACCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTG  >  NC_002947/5420308‑5420600
                                                                                                                                                |                                                                                                                                                    
aCCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCgg                                                                                                                                                  >  5:14694/1‑149 (MQ=255)
aCCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCgg                                                                                                                                                  >  4:170820/1‑149 (MQ=255)
 ccccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                 <  2:178502/149‑1 (MQ=255)
   ccGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCAGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGt                                                                                                                                               <  4:61336/149‑1 (MQ=255)
     ggTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                 >  8:3951/1‑145 (MQ=255)
     ggTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCggg                                                                                                                                                 <  7:3951/145‑1 (MQ=255)
     ggTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTAACCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGc                                                                                                                                             >  5:334537/1‑149 (MQ=255)
      gTAAGTATGCCGATCAGCGAAACATGTCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCGGCCTGGGGTgag                                                                                                                                            <  8:283880/149‑3 (MQ=255)
         aGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCtt                                                                                                                                         <  6:117670/149‑1 (MQ=255)
          ggATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTg                                                                                                                                        <  1:348629/149‑1 (MQ=255)
                   tatgCGAAACATGCCCGCCAATGCGCGCAGCAATCTTCGACTGCTGCAGGGTCGCCGGTCCGCCCACTTCAATCTGATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCTCCCCAGGCCGGGGTGCTGTTGTAGTTGATc                                                                                                                               <  8:88924/146‑1 (MQ=255)
                     aGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAg                                                                                                                             <  1:349522/149‑1 (MQ=255)
                      gCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAgg                                                                                                                            <  3:170406/149‑1 (MQ=255)
                      gCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAgg                                                                                                                            <  3:170820/149‑1 (MQ=255)
                       cGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggt                                                                                                                           >  7:55482/1‑149 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggtg                                                                                                                          <  7:328053/149‑1 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggtg                                                                                                                          <  6:105265/149‑1 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggtg                                                                                                                          <  2:246357/149‑1 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCGCACCCCGGCCTGGGGTgagaag                                                                                                                                         >  6:120301/1‑129 (MQ=255)
                        gAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCGCACCCCGGCCTGGGGTgagaag                                                                                                                                         <  5:120301/134‑6 (MQ=255)
                         aaaCATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAggtgg                                                                                                                         >  8:367613/1‑149 (MQ=255)
                             aTGCCCGCCAATGCGCGCAGCAATGATCGACTGCTCCAGCGTCGCCGGGCCTCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCACAGGCCGGGGTGCTCTTGTAGTTTATCAGGTGGTCgg                                                                                                                     <  3:114754/149‑1 (MQ=255)
                             aTGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGt                                                                                                                                       <  5:188729/131‑1 (MQ=255)
                             aTGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGt                                                                                                                                       >  6:188729/1‑131 (MQ=255)
                                cccGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCa                                                                                                                   <  5:338057/148‑1 (MQ=255)
                                       aTGCGCGCAGCAATCATCGACTGCTCCATCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTTCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGc                                                                                                           <  2:336777/149‑1 (MQ=255)
                                           gcgcAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTc                                                                                                       <  7:367613/149‑1 (MQ=255)
                                             gcagcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCCCTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAg                                                                                                     >  5:393245/1‑149 (MQ=255)
                                             gcagcaATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAg                                                                                                     <  6:159447/149‑1 (MQ=255)
                                                   atcatcGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTc                                                                                               <  7:36677/149‑1 (MQ=255)
                                                    tcatcGACTGCTCCAGCGTCGCCCGGCCCGCCACGTCAATCACAAGATCGCCACCACGGGTGCCAGTCAGCTCGCGCACCTTCTCACCCCCCGACCGGCTGCTCTTGGAGGTGATCAGGAGCTCGGCACCCACGGCCTGCAGGCGCTcc                                                                                              >  7:294155/1‑149 (MQ=255)
                                                      atcGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAg                                                                                            >  3:190325/1‑149 (MQ=255)
                                                               tCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATc                                                                                   >  4:198665/1‑149 (MQ=255)
                                                                 cAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGc                                                                                 <  1:237288/149‑1 (MQ=255)
                                                                         ccGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAg                                                                         >  5:230996/1‑149 (MQ=255)
                                                                           gggCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGt                                                                       <  4:71822/149‑1 (MQ=255)
                                                                             gccgccCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTgg                                                                     <  2:94330/149‑1 (MQ=255)
                                                                                   cACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGAGCa                                                               <  3:40250/149‑1 (MQ=255)
                                                                                          aTCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTcgc                                                        >  3:196283/1‑149 (MQ=255)
                                                                                           tCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTcgcg                                                       >  8:298509/1‑149 (MQ=255)
                                                                                              caTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTc                                                          >  3:342076/1‑143 (MQ=255)
                                                                                              caTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTc                                                          <  4:342076/143‑1 (MQ=255)
                                                                                              caTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgccc                                                    >  4:340528/1‑149 (MQ=255)
                                                                                              caTGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgccc                                                    >  7:17871/1‑149 (MQ=255)
                                                                                                tGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGcgcccgc                                                  >  8:3122/1‑149 (MQ=255)
                                                                                                      acacCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGAt                                                                 >  8:299432/1‑128 (MQ=255)
                                                                                                      acacCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGAt                                                                 <  7:299432/128‑1 (MQ=255)
                                                                                                        accacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTgg                                          >  8:195606/1‑149 (MQ=255)
                                                                                                        accacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTgg                                          <  3:340528/149‑1 (MQ=255)
                                                                                                          cacGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGc                                         >  8:32492/1‑148 (MQ=255)
                                                                                                           acGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCg                                        >  4:9772/1‑148 (MQ=255)
                                                                                                           acGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCg                                        >  8:8657/1‑148 (MQ=255)
                                                                                                             ggTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAAc                                     <  6:14694/149‑1 (MQ=255)
                                                                                                                   cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                               >  4:22909/1‑149 (MQ=255)
                                                                                                                   cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                               <  7:3122/149‑1 (MQ=255)
                                                                                                                   cAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAgg                               >  4:28103/1‑149 (MQ=255)
                                                                                                                    aGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAggg                              <  3:28103/149‑1 (MQ=255)
                                                                                                                    aGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAggg                              <  3:22909/149‑1 (MQ=255)
                                                                                                                            cgcgCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATc                      <  7:32492/149‑1 (MQ=255)
                                                                                                                             gcgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATc                      >  2:15387/1‑148 (MQ=255)
                                                                                                                              cgcACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGa                    <  7:8657/149‑1 (MQ=255)
                                                                                                                                 aCCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGaca                  >  5:170439/1‑148 (MQ=255)
                                                                                                                                   cTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCCCCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGacacac               >  6:354399/1‑149 (MQ=255)
                                                                                                                                   aagagcACCCCGGCCTGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTTGCATCGCTGGACGAGGTGGCGATAACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGacacac               >  4:126810/6‑149 (MQ=255)
                                                                                                                                      ctcACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACAccgc            >  5:233072/1‑149 (MQ=255)
                                                                                                                                           cccAGGCCGGGGTGCTCTTGTAGTAGATCAGGTTGTCGGCACCCAGGGCATTCATGCGCTCCAGCTTGCCATCGCAGGACGAGGTGGCGATCACGGTCGCGCACGACACCTTGGAGAACTGCAGGGCGAAAATCGACACACCGCCggaa       >  6:364029/1‑147 (MQ=255)
                                                                                                                                                gCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTg  <  8:55482/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ACCCCGGTAAGGATGCCGATCAGCGAAACATGCCCGCCAATGCGCGCAGCAATCATCGACTGCTCCAGCGTCGCCGGGCCGCCCACTTCAATCACATGATCGACACCACGGTTGCCAGTCAGCTCGCGCACCTTCTCACCCCAGGCCGGGGTGCTCTTGTAGTTGATCAGGTGGTCGGCACCCAGGGCCTTCAGGCGCTCCAGCTTGGCATCGCTGGACGAGGTGGCGATCACGGTCGCGCCCGCCAGCTTGGCGAACTGCAGGGCGAAAATCGACACACCGCCGGTACCCTG  >  NC_002947/5420308‑5420600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑