Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,463,400	T→A	8.5%	intergenic (‑51/+48)	PP_4799 ← / ← lipA	muramoyltetrapeptide carboxypeptidase/lipoyl synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,463,400	0	T	A	8.5%	229.0 / 10.9	82	intergenic (‑51/+48)	PP_4799/lipA	muramoyltetrapeptide carboxypeptidase/lipoyl synthase
Reads supporting (aligned to +/- strand):  ref base T (34/41);  new base A (3/4);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

AAGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAAC  >  NC_002947/5463254‑5463545                                                                                                                                                    |                                                                                                                                                   aaGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTg                                                                                                                                                  >  5:294136/1‑149 (MQ=255)  aGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGTAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTgg                                                                                                                                                 >  8:283698/1‑149 (MQ=255)  aGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTgg                                                                                                                                                 <  7:89476/149‑1 (MQ=255)    ccgcgccgcaCCCGCATCGGCGACAATGGCAAAACATGCGTTGGCCGGCATCGCGGCTGGCAGGGTGAGTTGGAAGGTTTTGGCTAACTACATCGCGTCTCCTTCCAGCGCATACGACACAAACAAAAATGCCGATAC‑CACCCtagcgc                                                                                                                                               >  2:290124/1‑144 (MQ=255)         ccgcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCAAGGgcggcat                                                                                                                                          <  5:68409/149‑5 (MQ=255)            cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCg                                                                                                                                       <  2:38225/149‑1 (MQ=255)               ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCG‑GCATCGGCa                                                                                                                                    <  7:139129/149‑1 (MQ=255)               ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCa                                                                                                                                    >  5:76326/1‑149 (MQ=255)               ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCAGATGC‑CGCCCTTGGGCGCGCATCGGCa                                                                                                                                    >  1:77099/1‑149 (MQ=255)                    ggggCGACAATGGCAAAACATGCGTTGGCCGGTAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCAGTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCt‑gt                                                                                                                              <  7:283698/149‑1 (MQ=255)                    ggggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGcg‑gcatcggcattttt                                                                                                                               <  7:83660/149‑5 (MQ=38)                    ggggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCt‑gt                                                                                                                              <  1:389136/149‑1 (MQ=255)                     gggCGTCAATGGCAATACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGGATCt‑gtg                                                                                                                             <  2:209074/149‑1 (MQ=255)                      ggCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTgg                                                                                                                            >  4:314622/1‑149 (MQ=255)                         gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                         >  6:62265/1‑134 (MQ=255)                         gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCAt                                                                                                                                         <  5:62265/134‑1 (MQ=255)                         gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTc                                                                                                                         >  5:300908/1‑149 (MQ=255)                            aaTGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGc                                                                                                                      >  7:142038/1‑149 (MQ=255)                            aaTGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGc                                                                                                                      >  6:225879/1‑149 (MQ=255)                              tGGCAAAACATGTGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGctc                                                                                                                    <  1:258603/149‑1 (MQ=255)                              tGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGctc                                                                                                                    >  5:209879/1‑149 (MQ=255)                                gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTa                                                                                                                  <  6:323177/149‑1 (MQ=255)                                 cAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAg                                                                                                                 >  3:43728/1‑149 (MQ=255)                                 cAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAg                                                                                                                 <  2:140779/149‑1 (MQ=255)                                  aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAgg                                                                                                                >  8:270665/1‑149 (MQ=255)                                     aCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTa                                                                                                             <  6:294136/149‑1 (MQ=255)                                     aCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTa                                                                                                             <  4:103755/149‑1 (MQ=255)                                       aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTa                                                                                                             >  2:329323/1‑147 (MQ=255)                                           gTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCa                                                                                                        >  8:62904/1‑148 (MQ=255)                                            ttGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATa                                                                                                      <  6:54566/149‑1 (MQ=255)                                            ttGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATa                                                                                                      <  8:142038/149‑1 (MQ=255)                                              ggCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAg                                                                                                     <  1:116408/148‑1 (MQ=255)                                               gCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCt                                                                                                   >  5:353871/1‑149 (MQ=255)                                                 cGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCt                                                                                                   >  3:138448/1‑147 (MQ=255)                                                       cgcgGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCtt                                                                                           <  3:314622/149‑1 (MQ=255)                                                            cTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCt                                                                                      <  5:225879/149‑1 (MQ=255)                                                               gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTg                                                                                    <  6:300908/148‑1 (MQ=255)                                                                cAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGc                                                                                  <  2:77099/149‑1 (MQ=255)                                                                    gggggaTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCg                                                                              <  4:295485/147‑1 (MQ=255)                                                                       ggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCTCGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTg                                                                            <  1:101857/149‑1 (MQ=255)                                                                       ggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTgg                                                                           <  6:209879/149‑1 (MQ=255)                                                                        gtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTggg                                                                          >  1:273656/1‑149 (MQ=255)                                                                        gtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTggg                                                                          >  2:294288/1‑149 (MQ=255)                                                                            gAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                      <  5:166629/149‑1 (MQ=255)                                                                            gAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCatg                                                                      >  7:306784/1‑149 (MQ=255)                                                                              tgtttttGGTGAAGTACATCGCGTCTCGTTTCAGTGCATTGGAAACAATCAAAAATGCCTTTGC‑CGCCGTTGGGCGCGTATCGGCATCT‑GTGGCTCCGTTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctgc                                                                     <  1:290124/145‑1 (MQ=255)                                                                               ggTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGTGCctgctg                                                                   >  8:319606/1‑149 (MQ=255)                                                                                     ggCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTACGCTCTAGGCTAGAGCATAGATCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGc                                                             >  7:317418/1‑149 (MQ=255)                                                                                       cgcAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGt                                                           <  4:237865/149‑1 (MQ=255)                                                                                            tACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTAc                                                      >  8:321803/1‑149 (MQ=255)                                                                                             aCATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTAc                                                      <  5:306584/148‑1 (MQ=255)                                                                                               aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                   <  6:42538/149‑1 (MQ=255)                                                                                               aTCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                   <  5:211069/149‑1 (MQ=255)                                                                                                      cTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCa                                            >  4:51857/1‑149 (MQ=255)                                                                                                      cTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCa                                            <  3:51857/149‑1 (MQ=255)                                                                                                        ccTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                           >  2:19269/1‑148 (MQ=255)                                                                                                          ttGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAg                                        >  8:211491/1‑149 (MQ=255)                                                                                                              atcgaaTTGGACACAAACAATAATGCCGATGC‑CGGCCTTGGGCGCGCATCGTCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTTGTCGGCGTGGTACGAAGGACGCACCAGCg                                      <  3:351006/142‑1 (MQ=255)                                                                                                              aGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCggg                                    >  1:270383/1‑149 (MQ=255)                                                                                                              aGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATATTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGCACGCACCAGCg                                      >  4:351006/1‑147 (MQ=255)                                                                                                                 gcATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCa                                 <  6:353871/149‑1 (MQ=255)                                                                                                                  cATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAg                                <  7:277370/149‑1 (MQ=255)                                                                                                                    ttGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGaa                              <  7:270665/149‑1 (MQ=255)                                                                                                                        acacAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCCCCAGCGGGCCAGAAGCaa                          >  7:213036/1‑149 (MQ=255)                                                                                                                        acacAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCaa                          <  5:366834/149‑1 (MQ=255)                                                                                                                          acaaacaaAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTg                                                                            <  8:220589/97‑1 (MQ=255)                                                                                                                          acaaacaaAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTg                                                                            >  7:220589/1‑97 (MQ=255)                                                                                                                            aaacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGtt                      >  7:119936/1‑149 (MQ=255)                                                                                                                             aacaaaAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTc                     >  1:42309/1‑149 (MQ=255)                                                                                                                                aaaaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCtt                   >  2:146385/1‑148 (MQ=255)                                                                                                                                aaaaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTg                  <  7:62904/149‑1 (MQ=255)                                                                                                                                 aaaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGa                 <  1:19269/149‑1 (MQ=255)                                                                                                                                  aaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTg                  >  1:216802/1‑147 (MQ=255)                                                                                                                                  aaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGaa                <  3:297172/149‑1 (MQ=255)                                                                                                                                  aaaTGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGaa                <  1:146385/149‑1 (MQ=255)                                                                                                                                     tgccgatgc‑cgCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGcc             <  2:216802/149‑1 (MQ=255)                                                                                                                                        cgatgcgcGCCCAAGGGCG‑GCATCGGCATTT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCAt          >  7:277330/1‑149 (MQ=255)                                                                                                                                         gatgcgcgccCAAGGGCG‑GCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACACACCAGCGGGCCAGaa                              >  4:331742/7‑128 (MQ=255)                                                                                                                                         gatgcgcgccCAAGGGCG‑GCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACACACCAGCGGGCCAGaa                              <  3:331742/122‑1 (MQ=255)                                                                                                                                          atgcgcgccCAAGGGCG‑GCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTTGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCt        >  6:28929/6‑149 (MQ=255)                                                                                                                                           tgc‑cgCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCGCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCtt       <  6:76326/149‑1 (MQ=255)                                                                                                                                               cgccCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTa    <  2:42309/149‑1 (MQ=255)                                                                                                                                                 cccTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAAc  >  6:301701/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AAGCCGCGCCGCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGC‑CGCCCTTGGGCGCGCATCGGCATCT‑GTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAAC  >  NC_002947/5463254‑5463545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑