Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,469,183 A→T 8.5% V272E (GTG→GAG)  mrdB ← murein transpeptidase factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,469,1830AT 8.5% 162.1 / 5.8 59V272E (GTG→GAG) mrdBmurein transpeptidase factor
Reads supporting (aligned to +/- strand):  ref base A (31/23);  new base T (2/3);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

AAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACGGGTCGCTT  >  NC_002947/5469038‑5469329
                                                                                                                                                 |                                                                                                                                                  
aaaaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACgg                                                                                                                                                 <  3:221883/149‑1 (MQ=255)
aaaaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACgg                                                                                                                                                 <  6:400497/149‑1 (MQ=255)
aaaaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACgg                                                                                                                                                 >  7:343511/1‑149 (MQ=255)
  aaaaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGc                                                                                                                                                >  4:311048/1‑148 (MQ=255)
    aaaGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgag                                                                                                                                             <  2:227588/149‑2 (MQ=255)
      aGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCgatga                                                                                                                                           <  5:317576/149‑1 (MQ=255)
       gTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTTGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgaggaa                                                                                                                                          <  5:88485/149‑2 (MQ=255)
          aTGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGatgatg                                                                                                                                         >  8:162108/1‑147 (MQ=255)
            ggTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCCTGCCGAATTCCTCGCCGAGCACGGCGATGAGGCAGt                                                                                                                                     >  7:242363/1‑149 (MQ=255)
              tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                                   <  3:361436/149‑1 (MQ=255)
              tCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCg                                                                                                                                   <  7:162108/149‑1 (MQ=255)
                aGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgt                                                                                                                                 <  3:311048/149‑1 (MQ=255)
                  gCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGgtgt                                                                                                                               <  7:121040/149‑1 (MQ=255)
                    tGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGAGAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgag                                                                                                                                             >  1:63526/1‑132 (MQ=255)
                    tGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGAGAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGTGCTCGGCgag                                                                                                                                             <  2:63526/133‑2 (MQ=255)
                        cGCAATCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACGAGGCCACGGCCGATCAGCTGCTGGTAGACAATCAGCAGCAGGCAGTTGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCttt                                                                                                                         <  5:284766/149‑1 (MQ=255)
                            agcagcTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCgg                                                                                                                     <  3:271984/149‑1 (MQ=255)
                                 cTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCAGGCaaa                                                                                                                 >  1:147457/1‑148 (MQ=255)
                                      cGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTc                                                                                                            >  2:243607/1‑148 (MQ=255)
                                      cGAACAGGGTCTGCGACTGGGCGGTGATCACCAGGCCACGGCAGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTcc                                                                                                           <  1:294938/149‑1 (MQ=255)
                                        aaCAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAg                                                                                                         >  4:348219/1‑149 (MQ=255)
                                              gTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGC‑‑‑GATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTg                                                                                                >  1:187733/1‑149 (MQ=255)
                                                    gcCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGt                                                                                             >  8:202191/1‑149 (MQ=255)
                                                      cTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGc                                                                                           >  3:72468/1‑149 (MQ=255)
                                                          gCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAag                                                                                       >  5:235534/1‑149 (MQ=255)
                                                              tGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAagcagc                                                                                   >  6:69904/1‑149 (MQ=255)
                                                                   aCCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCagccagcc                                                                              >  7:237486/1‑149 (MQ=255)
                                                                     cAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCtt                                                                            >  7:210571/1‑149 (MQ=255)
                                                                         ccACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGcc                                                                        <  8:343511/149‑1 (MQ=255)
                                                                                        agcagGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACcccgccc                                                           >  8:113307/1‑147 (MQ=255)
                                                                                             gTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTgg                                                                                                                             >  4:284885/1‑76 (MQ=255)
                                                                                               aGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATc                                                  >  8:188591/1‑149 (MQ=255)
                                                                                                gACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATc                                                  <  6:235534/148‑1 (MQ=255)
                                                                                                gACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGC‑‑‑GATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATcgcc                                               <  2:187733/148‑1 (MQ=255)
                                                                                                  cAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTg                                                                          >  1:364878/1‑122 (MQ=255)
                                                                                                  cAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTg                                                                          <  2:364878/122‑1 (MQ=255)
                                                                                                  cAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAaca                                                                   <  1:72102/129‑1 (MQ=255)
                                                                                                  cAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAaca                                                                   >  2:72102/1‑129 (MQ=255)
                                                                                                     tCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCgccgcc                                            >  1:258097/1‑149 (MQ=255)
                                                                                                      cagcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCt                                           >  2:97974/1‑149 (MQ=255)
                                                                                                       agcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCTAGCACGGCGATGATGAATTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGAAGCCAGCCTTTGCCAAACACCCCGACCGAGCCGATCgcagcc                                            >  4:131484/1‑147 (MQ=255)
                                                                                                       agcagcagGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCtt                                          <  5:69904/149‑1 (MQ=255)
                                                                                                            caggcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACt                                     <  7:188591/149‑1 (MQ=255)
                                                                                                               gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGga                                  >  8:322175/1‑149 (MQ=255)
                                                                                                               gcagATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGga                                  <  1:243607/149‑1 (MQ=255)
                                                                                                                 agATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGgat                                 >  8:134881/1‑148 (MQ=255)
                                                                                                                  gATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTg                                    >  1:181605/1‑144 (MQ=255)
                                                                                                                  gATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTg                                    <  2:181605/144‑1 (MQ=255)
                                                                                                                    tGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGatgatg                             <  7:202191/149‑1 (MQ=255)
                                                                                                                      ccTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGtt                           <  3:131484/149‑1 (MQ=255)
                                                                                                                       cTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTc                          >  2:308810/1‑149 (MQ=255)
                                                                                                                        tACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTc                          >  1:18926/1‑148 (MQ=255)
                                                                                                                          ccAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTcc                         <  3:348219/147‑1 (MQ=255)
                                                                                                                                  aaTTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTg                 >  7:4304/1‑147 (MQ=255)
                                                                                                                                  aaTTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTg                 <  8:4304/147‑1 (MQ=255)
                                                                                                                                      ccTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCCATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGACCa             >  3:213843/1‑147 (MQ=255)
                                                                                                                                        tCGCCGTGCTCGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACgg         >  3:177686/1‑149 (MQ=255)
                                                                                                                                        tCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACgg         >  3:50279/1‑149 (MQ=255)
                                                                                                                                               gCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCAGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACGGGTAGCtt  >  3:177301/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAAAAAAGTCATGGTCAGGCTGCCCGCAAGCAGCTTGCCGAACAGGGTCTGCGCCTGGGCGGTGATCACCAGGCCACGGCCGATCAGCAGCAGGTAGACAATCAGCAGCAGGCAGATGCCTACCAGGCCGAATTCCTCGCCGAGCACGGCGATGATGAAGTCGGTGTGGCTTTCCGGCAAAAAGTCCAGGTGCGACTGCGTGCCAAGCAGCCAGCCTTTGCCAAACACCCCGCCCGAGCCGATCGCCGCCTTGGACTGGATGATGTTCCAACCGGTGCCCAACGGGTCGCTT  >  NC_002947/5469038‑5469329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑